Job ID = 14521473 SRX = SRX8833543 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23255112 spots for SRR12333579/SRR12333579.sra Written 23255112 spots for SRR12333579/SRR12333579.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 23255112 reads; of these: 23255112 (100.00%) were unpaired; of these: 2735584 (11.76%) aligned 0 times 18063668 (77.68%) aligned exactly 1 time 2455860 (10.56%) aligned >1 times 88.24% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11754959 / 20519528 = 0.5729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:04: 1000000 INFO @ Sat, 15 Jan 2022 21:10:09: 2000000 INFO @ Sat, 15 Jan 2022 21:10:14: 3000000 INFO @ Sat, 15 Jan 2022 21:10:19: 4000000 INFO @ Sat, 15 Jan 2022 21:10:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:30: 6000000 INFO @ Sat, 15 Jan 2022 21:10:34: 1000000 INFO @ Sat, 15 Jan 2022 21:10:36: 7000000 INFO @ Sat, 15 Jan 2022 21:10:41: 2000000 INFO @ Sat, 15 Jan 2022 21:10:43: 8000000 INFO @ Sat, 15 Jan 2022 21:10:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:10:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:10:47: #1 total tags in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:10:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:48: #1 tags after filtering in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:10:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:10:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:10:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:48: 3000000 INFO @ Sat, 15 Jan 2022 21:10:55: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:02: 5000000 INFO @ Sat, 15 Jan 2022 21:11:05: 1000000 INFO @ Sat, 15 Jan 2022 21:11:09: 6000000 INFO @ Sat, 15 Jan 2022 21:11:12: 2000000 INFO @ Sat, 15 Jan 2022 21:11:16: 7000000 INFO @ Sat, 15 Jan 2022 21:11:18: 3000000 INFO @ Sat, 15 Jan 2022 21:11:23: 8000000 INFO @ Sat, 15 Jan 2022 21:11:25: 4000000 INFO @ Sat, 15 Jan 2022 21:11:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:11:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:11:28: #1 total tags in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:11:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:28: #1 tags after filtering in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:11:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:11:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:31: 5000000 INFO @ Sat, 15 Jan 2022 21:11:36: 6000000 INFO @ Sat, 15 Jan 2022 21:11:41: 7000000 INFO @ Sat, 15 Jan 2022 21:11:47: 8000000 INFO @ Sat, 15 Jan 2022 21:11:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:11:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:11:51: #1 total tags in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:11:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:51: #1 tags after filtering in treatment: 8764569 INFO @ Sat, 15 Jan 2022 21:11:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:11:51: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833543/SRX8833543.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。