Job ID = 14521471 SRX = SRX8833541 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45247547 spots for SRR12333577/SRR12333577.sra Written 45247547 spots for SRR12333577/SRR12333577.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:57 45247547 reads; of these: 45247547 (100.00%) were unpaired; of these: 2610629 (5.77%) aligned 0 times 38334733 (84.72%) aligned exactly 1 time 4302185 (9.51%) aligned >1 times 94.23% overall alignment rate Time searching: 00:08:57 Overall time: 00:08:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28471883 / 42636918 = 0.6678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:27:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:27:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:27:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:01: 1000000 INFO @ Sat, 15 Jan 2022 21:28:09: 2000000 INFO @ Sat, 15 Jan 2022 21:28:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:27: 4000000 INFO @ Sat, 15 Jan 2022 21:28:29: 1000000 INFO @ Sat, 15 Jan 2022 21:28:36: 5000000 INFO @ Sat, 15 Jan 2022 21:28:36: 2000000 INFO @ Sat, 15 Jan 2022 21:28:43: 3000000 INFO @ Sat, 15 Jan 2022 21:28:45: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:51: 4000000 INFO @ Sat, 15 Jan 2022 21:28:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:54: 7000000 INFO @ Sat, 15 Jan 2022 21:28:58: 5000000 INFO @ Sat, 15 Jan 2022 21:29:01: 1000000 INFO @ Sat, 15 Jan 2022 21:29:04: 8000000 INFO @ Sat, 15 Jan 2022 21:29:07: 6000000 INFO @ Sat, 15 Jan 2022 21:29:09: 2000000 INFO @ Sat, 15 Jan 2022 21:29:14: 9000000 INFO @ Sat, 15 Jan 2022 21:29:15: 7000000 INFO @ Sat, 15 Jan 2022 21:29:17: 3000000 INFO @ Sat, 15 Jan 2022 21:29:23: 10000000 INFO @ Sat, 15 Jan 2022 21:29:24: 8000000 INFO @ Sat, 15 Jan 2022 21:29:26: 4000000 INFO @ Sat, 15 Jan 2022 21:29:32: 9000000 INFO @ Sat, 15 Jan 2022 21:29:33: 11000000 INFO @ Sat, 15 Jan 2022 21:29:34: 5000000 INFO @ Sat, 15 Jan 2022 21:29:40: 10000000 INFO @ Sat, 15 Jan 2022 21:29:43: 6000000 INFO @ Sat, 15 Jan 2022 21:29:43: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:29:49: 11000000 INFO @ Sat, 15 Jan 2022 21:29:51: 7000000 INFO @ Sat, 15 Jan 2022 21:29:52: 13000000 INFO @ Sat, 15 Jan 2022 21:29:57: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:30:00: 8000000 INFO @ Sat, 15 Jan 2022 21:30:01: 14000000 INFO @ Sat, 15 Jan 2022 21:30:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:30:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:30:02: #1 total tags in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:02: #1 tags after filtering in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:30:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:30:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:30:05: 13000000 INFO @ Sat, 15 Jan 2022 21:30:07: 9000000 INFO @ Sat, 15 Jan 2022 21:30:13: 14000000 INFO @ Sat, 15 Jan 2022 21:30:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:30:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:30:14: #1 total tags in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:15: #1 tags after filtering in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:30:15: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:15: 10000000 INFO @ Sat, 15 Jan 2022 21:30:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:30:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:30:22: 11000000 INFO @ Sat, 15 Jan 2022 21:30:30: 12000000 INFO @ Sat, 15 Jan 2022 21:30:38: 13000000 INFO @ Sat, 15 Jan 2022 21:30:45: 14000000 INFO @ Sat, 15 Jan 2022 21:30:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:30:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:30:46: #1 total tags in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:47: #1 tags after filtering in treatment: 14165035 INFO @ Sat, 15 Jan 2022 21:30:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:30:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:30:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833541/SRX8833541.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling