Job ID = 14520095 SRX = SRX8829460 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 66473174 spots for SRR12329230/SRR12329230.sra Written 66473174 spots for SRR12329230/SRR12329230.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:57:05 66473174 reads; of these: 66473174 (100.00%) were paired; of these: 3572686 (5.37%) aligned concordantly 0 times 54149312 (81.46%) aligned concordantly exactly 1 time 8751176 (13.16%) aligned concordantly >1 times ---- 3572686 pairs aligned concordantly 0 times; of these: 1028216 (28.78%) aligned discordantly 1 time ---- 2544470 pairs aligned 0 times concordantly or discordantly; of these: 5088940 mates make up the pairs; of these: 4208426 (82.70%) aligned 0 times 427688 (8.40%) aligned exactly 1 time 452826 (8.90%) aligned >1 times 96.83% overall alignment rate Time searching: 00:57:05 Overall time: 00:57:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 45879211 / 63916453 = 0.7178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:43:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:43:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:43:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:43:41: 1000000 INFO @ Sat, 15 Jan 2022 19:43:45: 2000000 INFO @ Sat, 15 Jan 2022 19:43:49: 3000000 INFO @ Sat, 15 Jan 2022 19:43:54: 4000000 INFO @ Sat, 15 Jan 2022 19:43:58: 5000000 INFO @ Sat, 15 Jan 2022 19:44:02: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:44:07: 7000000 INFO @ Sat, 15 Jan 2022 19:44:10: 1000000 INFO @ Sat, 15 Jan 2022 19:44:11: 8000000 INFO @ Sat, 15 Jan 2022 19:44:15: 2000000 INFO @ Sat, 15 Jan 2022 19:44:16: 9000000 INFO @ Sat, 15 Jan 2022 19:44:19: 3000000 INFO @ Sat, 15 Jan 2022 19:44:20: 10000000 INFO @ Sat, 15 Jan 2022 19:44:24: 4000000 INFO @ Sat, 15 Jan 2022 19:44:25: 11000000 INFO @ Sat, 15 Jan 2022 19:44:29: 5000000 INFO @ Sat, 15 Jan 2022 19:44:30: 12000000 INFO @ Sat, 15 Jan 2022 19:44:33: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:34: 13000000 INFO @ Sat, 15 Jan 2022 19:44:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:44:38: 7000000 INFO @ Sat, 15 Jan 2022 19:44:39: 14000000 INFO @ Sat, 15 Jan 2022 19:44:41: 1000000 INFO @ Sat, 15 Jan 2022 19:44:43: 8000000 INFO @ Sat, 15 Jan 2022 19:44:44: 15000000 INFO @ Sat, 15 Jan 2022 19:44:47: 2000000 INFO @ Sat, 15 Jan 2022 19:44:48: 9000000 INFO @ Sat, 15 Jan 2022 19:44:49: 16000000 INFO @ Sat, 15 Jan 2022 19:44:52: 3000000 INFO @ Sat, 15 Jan 2022 19:44:53: 10000000 INFO @ Sat, 15 Jan 2022 19:44:54: 17000000 INFO @ Sat, 15 Jan 2022 19:44:58: 4000000 INFO @ Sat, 15 Jan 2022 19:44:58: 11000000 INFO @ Sat, 15 Jan 2022 19:44:59: 18000000 INFO @ Sat, 15 Jan 2022 19:45:03: 12000000 INFO @ Sat, 15 Jan 2022 19:45:03: 5000000 INFO @ Sat, 15 Jan 2022 19:45:03: 19000000 INFO @ Sat, 15 Jan 2022 19:45:07: 13000000 INFO @ Sat, 15 Jan 2022 19:45:08: 20000000 INFO @ Sat, 15 Jan 2022 19:45:08: 6000000 INFO @ Sat, 15 Jan 2022 19:45:12: 14000000 INFO @ Sat, 15 Jan 2022 19:45:13: 21000000 INFO @ Sat, 15 Jan 2022 19:45:14: 7000000 INFO @ Sat, 15 Jan 2022 19:45:17: 15000000 INFO @ Sat, 15 Jan 2022 19:45:18: 22000000 INFO @ Sat, 15 Jan 2022 19:45:19: 8000000 INFO @ Sat, 15 Jan 2022 19:45:22: 16000000 INFO @ Sat, 15 Jan 2022 19:45:23: 23000000 INFO @ Sat, 15 Jan 2022 19:45:25: 9000000 INFO @ Sat, 15 Jan 2022 19:45:27: 17000000 INFO @ Sat, 15 Jan 2022 19:45:27: 24000000 INFO @ Sat, 15 Jan 2022 19:45:30: 10000000 INFO @ Sat, 15 Jan 2022 19:45:32: 18000000 INFO @ Sat, 15 Jan 2022 19:45:32: 25000000 INFO @ Sat, 15 Jan 2022 19:45:35: 11000000 INFO @ Sat, 15 Jan 2022 19:45:36: 19000000 INFO @ Sat, 15 Jan 2022 19:45:37: 26000000 INFO @ Sat, 15 Jan 2022 19:45:41: 12000000 INFO @ Sat, 15 Jan 2022 19:45:41: 20000000 INFO @ Sat, 15 Jan 2022 19:45:42: 27000000 INFO @ Sat, 15 Jan 2022 19:45:46: 21000000 INFO @ Sat, 15 Jan 2022 19:45:46: 13000000 INFO @ Sat, 15 Jan 2022 19:45:47: 28000000 INFO @ Sat, 15 Jan 2022 19:45:51: 22000000 INFO @ Sat, 15 Jan 2022 19:45:51: 29000000 INFO @ Sat, 15 Jan 2022 19:45:52: 14000000 INFO @ Sat, 15 Jan 2022 19:45:56: 23000000 INFO @ Sat, 15 Jan 2022 19:45:56: 30000000 INFO @ Sat, 15 Jan 2022 19:45:57: 15000000 INFO @ Sat, 15 Jan 2022 19:46:00: 24000000 INFO @ Sat, 15 Jan 2022 19:46:01: 31000000 INFO @ Sat, 15 Jan 2022 19:46:02: 16000000 INFO @ Sat, 15 Jan 2022 19:46:05: 25000000 INFO @ Sat, 15 Jan 2022 19:46:06: 32000000 INFO @ Sat, 15 Jan 2022 19:46:08: 17000000 INFO @ Sat, 15 Jan 2022 19:46:10: 26000000 INFO @ Sat, 15 Jan 2022 19:46:10: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:46:13: 18000000 INFO @ Sat, 15 Jan 2022 19:46:15: 27000000 INFO @ Sat, 15 Jan 2022 19:46:15: 34000000 INFO @ Sat, 15 Jan 2022 19:46:19: 19000000 INFO @ Sat, 15 Jan 2022 19:46:19: 28000000 INFO @ Sat, 15 Jan 2022 19:46:20: 35000000 INFO @ Sat, 15 Jan 2022 19:46:24: 20000000 INFO @ Sat, 15 Jan 2022 19:46:24: 29000000 INFO @ Sat, 15 Jan 2022 19:46:25: 36000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:46:29: 30000000 INFO @ Sat, 15 Jan 2022 19:46:29: 21000000 INFO @ Sat, 15 Jan 2022 19:46:30: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:46:30: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:46:30: #1 total tags in treatment: 17734212 INFO @ Sat, 15 Jan 2022 19:46:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:46:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:46:30: #1 tags after filtering in treatment: 7298200 INFO @ Sat, 15 Jan 2022 19:46:30: #1 Redundant rate of treatment: 0.59 INFO @ Sat, 15 Jan 2022 19:46:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:46:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:46:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:46:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:46:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:46:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:46:34: 31000000 INFO @ Sat, 15 Jan 2022 19:46:35: 22000000 INFO @ Sat, 15 Jan 2022 19:46:38: 32000000 INFO @ Sat, 15 Jan 2022 19:46:40: 23000000 INFO @ Sat, 15 Jan 2022 19:46:43: 33000000 INFO @ Sat, 15 Jan 2022 19:46:45: 24000000 INFO @ Sat, 15 Jan 2022 19:46:48: 34000000 INFO @ Sat, 15 Jan 2022 19:46:51: 25000000 INFO @ Sat, 15 Jan 2022 19:46:52: 35000000 INFO @ Sat, 15 Jan 2022 19:46:56: 26000000 INFO @ Sat, 15 Jan 2022 19:46:57: 36000000 INFO @ Sat, 15 Jan 2022 19:47:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:47:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:47:01: #1 total tags in treatment: 17734212 INFO @ Sat, 15 Jan 2022 19:47:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:02: 27000000 INFO @ Sat, 15 Jan 2022 19:47:02: #1 tags after filtering in treatment: 7298200 INFO @ Sat, 15 Jan 2022 19:47:02: #1 Redundant rate of treatment: 0.59 INFO @ Sat, 15 Jan 2022 19:47:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:07: 28000000 INFO @ Sat, 15 Jan 2022 19:47:12: 29000000 INFO @ Sat, 15 Jan 2022 19:47:17: 30000000 INFO @ Sat, 15 Jan 2022 19:47:22: 31000000 INFO @ Sat, 15 Jan 2022 19:47:27: 32000000 INFO @ Sat, 15 Jan 2022 19:47:33: 33000000 INFO @ Sat, 15 Jan 2022 19:47:38: 34000000 INFO @ Sat, 15 Jan 2022 19:47:43: 35000000 INFO @ Sat, 15 Jan 2022 19:47:48: 36000000 INFO @ Sat, 15 Jan 2022 19:47:53: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:47:53: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:47:53: #1 total tags in treatment: 17734212 INFO @ Sat, 15 Jan 2022 19:47:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:53: #1 tags after filtering in treatment: 7298200 INFO @ Sat, 15 Jan 2022 19:47:53: #1 Redundant rate of treatment: 0.59 INFO @ Sat, 15 Jan 2022 19:47:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829460/SRX8829460.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling