Job ID = 14520011 SRX = SRX8829459 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 59683727 spots for SRR12329229/SRR12329229.sra Written 59683727 spots for SRR12329229/SRR12329229.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:48:02 59683727 reads; of these: 59683727 (100.00%) were paired; of these: 3365321 (5.64%) aligned concordantly 0 times 47900512 (80.26%) aligned concordantly exactly 1 time 8417894 (14.10%) aligned concordantly >1 times ---- 3365321 pairs aligned concordantly 0 times; of these: 1091053 (32.42%) aligned discordantly 1 time ---- 2274268 pairs aligned 0 times concordantly or discordantly; of these: 4548536 mates make up the pairs; of these: 3386152 (74.44%) aligned 0 times 617771 (13.58%) aligned exactly 1 time 544613 (11.97%) aligned >1 times 97.16% overall alignment rate Time searching: 00:48:02 Overall time: 00:48:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 30476769 / 57398359 = 0.5310 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:19: 1000000 INFO @ Sat, 15 Jan 2022 19:33:24: 2000000 INFO @ Sat, 15 Jan 2022 19:33:30: 3000000 INFO @ Sat, 15 Jan 2022 19:33:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:41: 5000000 INFO @ Sat, 15 Jan 2022 19:33:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:47: 6000000 INFO @ Sat, 15 Jan 2022 19:33:49: 1000000 INFO @ Sat, 15 Jan 2022 19:33:53: 7000000 INFO @ Sat, 15 Jan 2022 19:33:54: 2000000 INFO @ Sat, 15 Jan 2022 19:33:58: 8000000 INFO @ Sat, 15 Jan 2022 19:33:58: 3000000 INFO @ Sat, 15 Jan 2022 19:34:03: 4000000 INFO @ Sat, 15 Jan 2022 19:34:04: 9000000 INFO @ Sat, 15 Jan 2022 19:34:08: 5000000 INFO @ Sat, 15 Jan 2022 19:34:09: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:13: 6000000 INFO @ Sat, 15 Jan 2022 19:34:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:14: 11000000 INFO @ Sat, 15 Jan 2022 19:34:18: 7000000 INFO @ Sat, 15 Jan 2022 19:34:20: 1000000 INFO @ Sat, 15 Jan 2022 19:34:20: 12000000 INFO @ Sat, 15 Jan 2022 19:34:23: 8000000 INFO @ Sat, 15 Jan 2022 19:34:25: 13000000 INFO @ Sat, 15 Jan 2022 19:34:26: 2000000 INFO @ Sat, 15 Jan 2022 19:34:28: 9000000 INFO @ Sat, 15 Jan 2022 19:34:31: 14000000 INFO @ Sat, 15 Jan 2022 19:34:31: 3000000 INFO @ Sat, 15 Jan 2022 19:34:33: 10000000 INFO @ Sat, 15 Jan 2022 19:34:36: 15000000 INFO @ Sat, 15 Jan 2022 19:34:37: 4000000 INFO @ Sat, 15 Jan 2022 19:34:39: 11000000 INFO @ Sat, 15 Jan 2022 19:34:42: 16000000 INFO @ Sat, 15 Jan 2022 19:34:43: 12000000 INFO @ Sat, 15 Jan 2022 19:34:44: 5000000 INFO @ Sat, 15 Jan 2022 19:34:48: 17000000 INFO @ Sat, 15 Jan 2022 19:34:48: 13000000 INFO @ Sat, 15 Jan 2022 19:34:50: 6000000 INFO @ Sat, 15 Jan 2022 19:34:53: 14000000 INFO @ Sat, 15 Jan 2022 19:34:54: 18000000 INFO @ Sat, 15 Jan 2022 19:34:57: 7000000 INFO @ Sat, 15 Jan 2022 19:34:58: 15000000 INFO @ Sat, 15 Jan 2022 19:34:59: 19000000 INFO @ Sat, 15 Jan 2022 19:35:02: 8000000 INFO @ Sat, 15 Jan 2022 19:35:03: 16000000 INFO @ Sat, 15 Jan 2022 19:35:05: 20000000 INFO @ Sat, 15 Jan 2022 19:35:08: 17000000 INFO @ Sat, 15 Jan 2022 19:35:08: 9000000 INFO @ Sat, 15 Jan 2022 19:35:11: 21000000 INFO @ Sat, 15 Jan 2022 19:35:13: 18000000 INFO @ Sat, 15 Jan 2022 19:35:14: 10000000 INFO @ Sat, 15 Jan 2022 19:35:17: 22000000 INFO @ Sat, 15 Jan 2022 19:35:19: 19000000 INFO @ Sat, 15 Jan 2022 19:35:20: 11000000 INFO @ Sat, 15 Jan 2022 19:35:23: 23000000 INFO @ Sat, 15 Jan 2022 19:35:24: 20000000 INFO @ Sat, 15 Jan 2022 19:35:26: 12000000 INFO @ Sat, 15 Jan 2022 19:35:28: 24000000 INFO @ Sat, 15 Jan 2022 19:35:29: 21000000 INFO @ Sat, 15 Jan 2022 19:35:32: 13000000 INFO @ Sat, 15 Jan 2022 19:35:34: 25000000 INFO @ Sat, 15 Jan 2022 19:35:34: 22000000 INFO @ Sat, 15 Jan 2022 19:35:38: 14000000 INFO @ Sat, 15 Jan 2022 19:35:40: 23000000 INFO @ Sat, 15 Jan 2022 19:35:40: 26000000 INFO @ Sat, 15 Jan 2022 19:35:44: 15000000 INFO @ Sat, 15 Jan 2022 19:35:45: 24000000 INFO @ Sat, 15 Jan 2022 19:35:45: 27000000 INFO @ Sat, 15 Jan 2022 19:35:50: 16000000 INFO @ Sat, 15 Jan 2022 19:35:50: 25000000 INFO @ Sat, 15 Jan 2022 19:35:51: 28000000 INFO @ Sat, 15 Jan 2022 19:35:55: 26000000 INFO @ Sat, 15 Jan 2022 19:35:55: 17000000 INFO @ Sat, 15 Jan 2022 19:35:56: 29000000 INFO @ Sat, 15 Jan 2022 19:36:00: 27000000 INFO @ Sat, 15 Jan 2022 19:36:02: 18000000 INFO @ Sat, 15 Jan 2022 19:36:02: 30000000 INFO @ Sat, 15 Jan 2022 19:36:05: 28000000 INFO @ Sat, 15 Jan 2022 19:36:08: 19000000 INFO @ Sat, 15 Jan 2022 19:36:08: 31000000 INFO @ Sat, 15 Jan 2022 19:36:10: 29000000 INFO @ Sat, 15 Jan 2022 19:36:13: 32000000 INFO @ Sat, 15 Jan 2022 19:36:14: 20000000 INFO @ Sat, 15 Jan 2022 19:36:15: 30000000 INFO @ Sat, 15 Jan 2022 19:36:19: 33000000 INFO @ Sat, 15 Jan 2022 19:36:19: 31000000 INFO @ Sat, 15 Jan 2022 19:36:20: 21000000 INFO @ Sat, 15 Jan 2022 19:36:24: 32000000 INFO @ Sat, 15 Jan 2022 19:36:25: 34000000 INFO @ Sat, 15 Jan 2022 19:36:27: 22000000 INFO @ Sat, 15 Jan 2022 19:36:29: 33000000 INFO @ Sat, 15 Jan 2022 19:36:31: 35000000 INFO @ Sat, 15 Jan 2022 19:36:33: 23000000 INFO @ Sat, 15 Jan 2022 19:36:33: 34000000 INFO @ Sat, 15 Jan 2022 19:36:37: 36000000 INFO @ Sat, 15 Jan 2022 19:36:38: 35000000 INFO @ Sat, 15 Jan 2022 19:36:40: 24000000 INFO @ Sat, 15 Jan 2022 19:36:42: 37000000 INFO @ Sat, 15 Jan 2022 19:36:43: 36000000 INFO @ Sat, 15 Jan 2022 19:36:46: 25000000 INFO @ Sat, 15 Jan 2022 19:36:48: 38000000 INFO @ Sat, 15 Jan 2022 19:36:48: 37000000 INFO @ Sat, 15 Jan 2022 19:36:52: 26000000 INFO @ Sat, 15 Jan 2022 19:36:53: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:36:54: 39000000 INFO @ Sat, 15 Jan 2022 19:36:57: 39000000 INFO @ Sat, 15 Jan 2022 19:36:58: 27000000 INFO @ Sat, 15 Jan 2022 19:36:59: 40000000 INFO @ Sat, 15 Jan 2022 19:37:02: 40000000 INFO @ Sat, 15 Jan 2022 19:37:04: 28000000 INFO @ Sat, 15 Jan 2022 19:37:05: 41000000 INFO @ Sat, 15 Jan 2022 19:37:07: 41000000 INFO @ Sat, 15 Jan 2022 19:37:11: 29000000 INFO @ Sat, 15 Jan 2022 19:37:11: 42000000 INFO @ Sat, 15 Jan 2022 19:37:11: 42000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:37:16: 43000000 INFO @ Sat, 15 Jan 2022 19:37:17: 43000000 INFO @ Sat, 15 Jan 2022 19:37:17: 30000000 INFO @ Sat, 15 Jan 2022 19:37:21: 44000000 INFO @ Sat, 15 Jan 2022 19:37:22: 44000000 INFO @ Sat, 15 Jan 2022 19:37:23: 31000000 INFO @ Sat, 15 Jan 2022 19:37:26: 45000000 INFO @ Sat, 15 Jan 2022 19:37:28: 45000000 INFO @ Sat, 15 Jan 2022 19:37:29: 32000000 INFO @ Sat, 15 Jan 2022 19:37:31: 46000000 INFO @ Sat, 15 Jan 2022 19:37:33: 46000000 INFO @ Sat, 15 Jan 2022 19:37:35: 33000000 INFO @ Sat, 15 Jan 2022 19:37:36: 47000000 INFO @ Sat, 15 Jan 2022 19:37:38: 47000000 INFO @ Sat, 15 Jan 2022 19:37:41: 34000000 INFO @ Sat, 15 Jan 2022 19:37:42: 48000000 INFO @ Sat, 15 Jan 2022 19:37:44: 48000000 INFO @ Sat, 15 Jan 2022 19:37:47: 35000000 INFO @ Sat, 15 Jan 2022 19:37:47: 49000000 INFO @ Sat, 15 Jan 2022 19:37:49: 49000000 INFO @ Sat, 15 Jan 2022 19:37:52: 50000000 INFO @ Sat, 15 Jan 2022 19:37:53: 36000000 INFO @ Sat, 15 Jan 2022 19:37:54: 50000000 INFO @ Sat, 15 Jan 2022 19:37:58: 51000000 INFO @ Sat, 15 Jan 2022 19:37:59: 37000000 INFO @ Sat, 15 Jan 2022 19:38:00: 51000000 INFO @ Sat, 15 Jan 2022 19:38:03: 52000000 INFO @ Sat, 15 Jan 2022 19:38:04: 38000000 INFO @ Sat, 15 Jan 2022 19:38:05: 52000000 INFO @ Sat, 15 Jan 2022 19:38:08: 53000000 INFO @ Sat, 15 Jan 2022 19:38:10: 53000000 INFO @ Sat, 15 Jan 2022 19:38:11: 39000000 INFO @ Sat, 15 Jan 2022 19:38:13: 54000000 INFO @ Sat, 15 Jan 2022 19:38:16: 54000000 INFO @ Sat, 15 Jan 2022 19:38:17: 40000000 INFO @ Sat, 15 Jan 2022 19:38:19: 55000000 INFO @ Sat, 15 Jan 2022 19:38:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:38:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:38:19: #1 total tags in treatment: 26328343 INFO @ Sat, 15 Jan 2022 19:38:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:20: #1 tags after filtering in treatment: 8916924 INFO @ Sat, 15 Jan 2022 19:38:20: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:38:20: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:38:21: 55000000 INFO @ Sat, 15 Jan 2022 19:38:22: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:38:22: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:38:22: #1 total tags in treatment: 26328343 INFO @ Sat, 15 Jan 2022 19:38:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:22: 41000000 INFO @ Sat, 15 Jan 2022 19:38:22: #1 tags after filtering in treatment: 8916924 INFO @ Sat, 15 Jan 2022 19:38:22: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:38:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:38:27: 42000000 INFO @ Sat, 15 Jan 2022 19:38:32: 43000000 INFO @ Sat, 15 Jan 2022 19:38:36: 44000000 INFO @ Sat, 15 Jan 2022 19:38:41: 45000000 INFO @ Sat, 15 Jan 2022 19:38:46: 46000000 INFO @ Sat, 15 Jan 2022 19:38:51: 47000000 INFO @ Sat, 15 Jan 2022 19:38:56: 48000000 INFO @ Sat, 15 Jan 2022 19:39:01: 49000000 INFO @ Sat, 15 Jan 2022 19:39:06: 50000000 INFO @ Sat, 15 Jan 2022 19:39:12: 51000000 INFO @ Sat, 15 Jan 2022 19:39:18: 52000000 INFO @ Sat, 15 Jan 2022 19:39:24: 53000000 INFO @ Sat, 15 Jan 2022 19:39:30: 54000000 INFO @ Sat, 15 Jan 2022 19:39:36: 55000000 INFO @ Sat, 15 Jan 2022 19:39:37: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:39:37: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:39:37: #1 total tags in treatment: 26328343 INFO @ Sat, 15 Jan 2022 19:39:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:38: #1 tags after filtering in treatment: 8916924 INFO @ Sat, 15 Jan 2022 19:39:38: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 19:39:38: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829459/SRX8829459.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling