Job ID = 14520010 SRX = SRX8829458 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 73896222 spots for SRR12329252/SRR12329252.sra Written 73896222 spots for SRR12329252/SRR12329252.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:00:49 73896222 reads; of these: 73896222 (100.00%) were paired; of these: 3966288 (5.37%) aligned concordantly 0 times 58311665 (78.91%) aligned concordantly exactly 1 time 11618269 (15.72%) aligned concordantly >1 times ---- 3966288 pairs aligned concordantly 0 times; of these: 1135213 (28.62%) aligned discordantly 1 time ---- 2831075 pairs aligned 0 times concordantly or discordantly; of these: 5662150 mates make up the pairs; of these: 4473047 (79.00%) aligned 0 times 516938 (9.13%) aligned exactly 1 time 672165 (11.87%) aligned >1 times 96.97% overall alignment rate Time searching: 01:00:49 Overall time: 01:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 47249628 / 71053476 = 0.6650 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:47: 1000000 INFO @ Sat, 15 Jan 2022 19:49:55: 2000000 INFO @ Sat, 15 Jan 2022 19:50:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:12: 4000000 INFO @ Sat, 15 Jan 2022 19:50:16: 1000000 INFO @ Sat, 15 Jan 2022 19:50:21: 5000000 INFO @ Sat, 15 Jan 2022 19:50:25: 2000000 INFO @ Sat, 15 Jan 2022 19:50:29: 6000000 INFO @ Sat, 15 Jan 2022 19:50:34: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:38: 7000000 INFO @ Sat, 15 Jan 2022 19:50:43: 4000000 INFO @ Sat, 15 Jan 2022 19:50:46: 8000000 INFO @ Sat, 15 Jan 2022 19:50:46: 1000000 INFO @ Sat, 15 Jan 2022 19:50:52: 5000000 INFO @ Sat, 15 Jan 2022 19:50:55: 9000000 INFO @ Sat, 15 Jan 2022 19:50:56: 2000000 INFO @ Sat, 15 Jan 2022 19:51:02: 6000000 INFO @ Sat, 15 Jan 2022 19:51:04: 10000000 INFO @ Sat, 15 Jan 2022 19:51:05: 3000000 INFO @ Sat, 15 Jan 2022 19:51:11: 7000000 INFO @ Sat, 15 Jan 2022 19:51:12: 11000000 INFO @ Sat, 15 Jan 2022 19:51:15: 4000000 INFO @ Sat, 15 Jan 2022 19:51:20: 8000000 INFO @ Sat, 15 Jan 2022 19:51:21: 12000000 INFO @ Sat, 15 Jan 2022 19:51:24: 5000000 INFO @ Sat, 15 Jan 2022 19:51:29: 9000000 INFO @ Sat, 15 Jan 2022 19:51:30: 13000000 INFO @ Sat, 15 Jan 2022 19:51:33: 6000000 INFO @ Sat, 15 Jan 2022 19:51:38: 10000000 INFO @ Sat, 15 Jan 2022 19:51:39: 14000000 INFO @ Sat, 15 Jan 2022 19:51:42: 7000000 INFO @ Sat, 15 Jan 2022 19:51:47: 11000000 INFO @ Sat, 15 Jan 2022 19:51:47: 15000000 INFO @ Sat, 15 Jan 2022 19:51:51: 8000000 INFO @ Sat, 15 Jan 2022 19:51:56: 16000000 INFO @ Sat, 15 Jan 2022 19:51:57: 12000000 INFO @ Sat, 15 Jan 2022 19:52:00: 9000000 INFO @ Sat, 15 Jan 2022 19:52:05: 17000000 INFO @ Sat, 15 Jan 2022 19:52:06: 13000000 INFO @ Sat, 15 Jan 2022 19:52:10: 10000000 INFO @ Sat, 15 Jan 2022 19:52:13: 18000000 INFO @ Sat, 15 Jan 2022 19:52:16: 14000000 INFO @ Sat, 15 Jan 2022 19:52:19: 11000000 INFO @ Sat, 15 Jan 2022 19:52:22: 19000000 INFO @ Sat, 15 Jan 2022 19:52:25: 15000000 INFO @ Sat, 15 Jan 2022 19:52:28: 12000000 INFO @ Sat, 15 Jan 2022 19:52:31: 20000000 INFO @ Sat, 15 Jan 2022 19:52:35: 16000000 INFO @ Sat, 15 Jan 2022 19:52:38: 13000000 INFO @ Sat, 15 Jan 2022 19:52:40: 21000000 INFO @ Sat, 15 Jan 2022 19:52:44: 17000000 INFO @ Sat, 15 Jan 2022 19:52:47: 14000000 INFO @ Sat, 15 Jan 2022 19:52:49: 22000000 INFO @ Sat, 15 Jan 2022 19:52:53: 18000000 INFO @ Sat, 15 Jan 2022 19:52:56: 15000000 INFO @ Sat, 15 Jan 2022 19:52:57: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:53:02: 19000000 INFO @ Sat, 15 Jan 2022 19:53:05: 16000000 INFO @ Sat, 15 Jan 2022 19:53:06: 24000000 INFO @ Sat, 15 Jan 2022 19:53:11: 20000000 INFO @ Sat, 15 Jan 2022 19:53:14: 17000000 INFO @ Sat, 15 Jan 2022 19:53:15: 25000000 INFO @ Sat, 15 Jan 2022 19:53:21: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:53:23: 18000000 INFO @ Sat, 15 Jan 2022 19:53:24: 26000000 INFO @ Sat, 15 Jan 2022 19:53:30: 22000000 INFO @ Sat, 15 Jan 2022 19:53:32: 19000000 INFO @ Sat, 15 Jan 2022 19:53:32: 27000000 INFO @ Sat, 15 Jan 2022 19:53:39: 23000000 INFO @ Sat, 15 Jan 2022 19:53:41: 28000000 INFO @ Sat, 15 Jan 2022 19:53:42: 20000000 INFO @ Sat, 15 Jan 2022 19:53:49: 24000000 INFO @ Sat, 15 Jan 2022 19:53:50: 29000000 INFO @ Sat, 15 Jan 2022 19:53:51: 21000000 INFO @ Sat, 15 Jan 2022 19:53:58: 25000000 INFO @ Sat, 15 Jan 2022 19:53:59: 30000000 INFO @ Sat, 15 Jan 2022 19:54:00: 22000000 INFO @ Sat, 15 Jan 2022 19:54:07: 26000000 INFO @ Sat, 15 Jan 2022 19:54:08: 31000000 INFO @ Sat, 15 Jan 2022 19:54:10: 23000000 INFO @ Sat, 15 Jan 2022 19:54:17: 27000000 INFO @ Sat, 15 Jan 2022 19:54:17: 32000000 INFO @ Sat, 15 Jan 2022 19:54:19: 24000000 INFO @ Sat, 15 Jan 2022 19:54:25: 33000000 INFO @ Sat, 15 Jan 2022 19:54:26: 28000000 INFO @ Sat, 15 Jan 2022 19:54:29: 25000000 INFO @ Sat, 15 Jan 2022 19:54:34: 34000000 INFO @ Sat, 15 Jan 2022 19:54:35: 29000000 INFO @ Sat, 15 Jan 2022 19:54:38: 26000000 INFO @ Sat, 15 Jan 2022 19:54:43: 35000000 INFO @ Sat, 15 Jan 2022 19:54:45: 30000000 INFO @ Sat, 15 Jan 2022 19:54:47: 27000000 INFO @ Sat, 15 Jan 2022 19:54:52: 36000000 INFO @ Sat, 15 Jan 2022 19:54:54: 31000000 INFO @ Sat, 15 Jan 2022 19:54:57: 28000000 INFO @ Sat, 15 Jan 2022 19:55:00: 37000000 INFO @ Sat, 15 Jan 2022 19:55:04: 32000000 INFO @ Sat, 15 Jan 2022 19:55:06: 29000000 INFO @ Sat, 15 Jan 2022 19:55:09: 38000000 INFO @ Sat, 15 Jan 2022 19:55:13: 33000000 INFO @ Sat, 15 Jan 2022 19:55:15: 30000000 INFO @ Sat, 15 Jan 2022 19:55:18: 39000000 INFO @ Sat, 15 Jan 2022 19:55:22: 34000000 INFO @ Sat, 15 Jan 2022 19:55:25: 31000000 INFO @ Sat, 15 Jan 2022 19:55:26: 40000000 INFO @ Sat, 15 Jan 2022 19:55:32: 35000000 INFO @ Sat, 15 Jan 2022 19:55:34: 32000000 INFO @ Sat, 15 Jan 2022 19:55:35: 41000000 INFO @ Sat, 15 Jan 2022 19:55:41: 36000000 INFO @ Sat, 15 Jan 2022 19:55:44: 33000000 INFO @ Sat, 15 Jan 2022 19:55:44: 42000000 INFO @ Sat, 15 Jan 2022 19:55:50: 37000000 INFO @ Sat, 15 Jan 2022 19:55:53: 43000000 INFO @ Sat, 15 Jan 2022 19:55:53: 34000000 INFO @ Sat, 15 Jan 2022 19:56:00: 38000000 INFO @ Sat, 15 Jan 2022 19:56:01: 44000000 INFO @ Sat, 15 Jan 2022 19:56:02: 35000000 INFO @ Sat, 15 Jan 2022 19:56:09: 39000000 INFO @ Sat, 15 Jan 2022 19:56:10: 45000000 INFO @ Sat, 15 Jan 2022 19:56:12: 36000000 INFO @ Sat, 15 Jan 2022 19:56:18: 46000000 INFO @ Sat, 15 Jan 2022 19:56:18: 40000000 INFO @ Sat, 15 Jan 2022 19:56:21: 37000000 INFO @ Sat, 15 Jan 2022 19:56:27: 47000000 INFO @ Sat, 15 Jan 2022 19:56:28: 41000000 INFO @ Sat, 15 Jan 2022 19:56:31: 38000000 INFO @ Sat, 15 Jan 2022 19:56:36: 48000000 INFO @ Sat, 15 Jan 2022 19:56:37: 42000000 INFO @ Sat, 15 Jan 2022 19:56:40: 39000000 INFO @ Sat, 15 Jan 2022 19:56:43: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:56:43: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:56:43: #1 total tags in treatment: 23364995 INFO @ Sat, 15 Jan 2022 19:56:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:43: #1 tags after filtering in treatment: 8281064 INFO @ Sat, 15 Jan 2022 19:56:43: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 19:56:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:56:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:56:46: 43000000 INFO @ Sat, 15 Jan 2022 19:56:49: 40000000 INFO @ Sat, 15 Jan 2022 19:56:56: 44000000 INFO @ Sat, 15 Jan 2022 19:56:58: 41000000 INFO @ Sat, 15 Jan 2022 19:57:05: 45000000 INFO @ Sat, 15 Jan 2022 19:57:08: 42000000 INFO @ Sat, 15 Jan 2022 19:57:14: 46000000 INFO @ Sat, 15 Jan 2022 19:57:17: 43000000 INFO @ Sat, 15 Jan 2022 19:57:24: 47000000 INFO @ Sat, 15 Jan 2022 19:57:26: 44000000 INFO @ Sat, 15 Jan 2022 19:57:33: 48000000 INFO @ Sat, 15 Jan 2022 19:57:35: 45000000 INFO @ Sat, 15 Jan 2022 19:57:40: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:57:40: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:57:40: #1 total tags in treatment: 23364995 INFO @ Sat, 15 Jan 2022 19:57:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:57:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:57:41: #1 tags after filtering in treatment: 8281064 INFO @ Sat, 15 Jan 2022 19:57:41: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 19:57:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:57:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:57:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:57:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:57:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:57:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:57:44: 46000000 INFO @ Sat, 15 Jan 2022 19:57:53: 47000000 INFO @ Sat, 15 Jan 2022 19:58:02: 48000000 INFO @ Sat, 15 Jan 2022 19:58:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:58:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:58:09: #1 total tags in treatment: 23364995 INFO @ Sat, 15 Jan 2022 19:58:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:58:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:58:10: #1 tags after filtering in treatment: 8281064 INFO @ Sat, 15 Jan 2022 19:58:10: #1 Redundant rate of treatment: 0.65 INFO @ Sat, 15 Jan 2022 19:58:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:58:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:58:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:58:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:58:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:58:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829458/SRX8829458.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling