Job ID = 14520009 SRX = SRX8829457 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 54248705 spots for SRR12329251/SRR12329251.sra Written 54248705 spots for SRR12329251/SRR12329251.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:46:27 54248705 reads; of these: 54248705 (100.00%) were paired; of these: 2511191 (4.63%) aligned concordantly 0 times 43046930 (79.35%) aligned concordantly exactly 1 time 8690584 (16.02%) aligned concordantly >1 times ---- 2511191 pairs aligned concordantly 0 times; of these: 596018 (23.73%) aligned discordantly 1 time ---- 1915173 pairs aligned 0 times concordantly or discordantly; of these: 3830346 mates make up the pairs; of these: 2988408 (78.02%) aligned 0 times 387600 (10.12%) aligned exactly 1 time 454338 (11.86%) aligned >1 times 97.25% overall alignment rate Time searching: 00:46:27 Overall time: 00:46:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 29711840 / 52322833 = 0.5679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:27:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:27:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:27:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:27:53: 1000000 INFO @ Sat, 15 Jan 2022 19:28:02: 2000000 INFO @ Sat, 15 Jan 2022 19:28:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:18: 4000000 INFO @ Sat, 15 Jan 2022 19:28:24: 1000000 INFO @ Sat, 15 Jan 2022 19:28:27: 5000000 INFO @ Sat, 15 Jan 2022 19:28:32: 2000000 INFO @ Sat, 15 Jan 2022 19:28:35: 6000000 INFO @ Sat, 15 Jan 2022 19:28:41: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:44: 7000000 INFO @ Sat, 15 Jan 2022 19:28:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:50: 4000000 INFO @ Sat, 15 Jan 2022 19:28:52: 8000000 INFO @ Sat, 15 Jan 2022 19:28:54: 1000000 INFO @ Sat, 15 Jan 2022 19:28:59: 5000000 INFO @ Sat, 15 Jan 2022 19:29:01: 9000000 INFO @ Sat, 15 Jan 2022 19:29:04: 2000000 INFO @ Sat, 15 Jan 2022 19:29:08: 6000000 INFO @ Sat, 15 Jan 2022 19:29:10: 10000000 INFO @ Sat, 15 Jan 2022 19:29:14: 3000000 INFO @ Sat, 15 Jan 2022 19:29:17: 7000000 INFO @ Sat, 15 Jan 2022 19:29:19: 11000000 INFO @ Sat, 15 Jan 2022 19:29:23: 4000000 INFO @ Sat, 15 Jan 2022 19:29:27: 8000000 INFO @ Sat, 15 Jan 2022 19:29:28: 12000000 INFO @ Sat, 15 Jan 2022 19:29:33: 5000000 INFO @ Sat, 15 Jan 2022 19:29:36: 9000000 INFO @ Sat, 15 Jan 2022 19:29:36: 13000000 INFO @ Sat, 15 Jan 2022 19:29:43: 6000000 INFO @ Sat, 15 Jan 2022 19:29:44: 10000000 INFO @ Sat, 15 Jan 2022 19:29:45: 14000000 INFO @ Sat, 15 Jan 2022 19:29:52: 7000000 INFO @ Sat, 15 Jan 2022 19:29:53: 11000000 INFO @ Sat, 15 Jan 2022 19:29:54: 15000000 INFO @ Sat, 15 Jan 2022 19:30:02: 8000000 INFO @ Sat, 15 Jan 2022 19:30:02: 16000000 INFO @ Sat, 15 Jan 2022 19:30:03: 12000000 INFO @ Sat, 15 Jan 2022 19:30:11: 17000000 INFO @ Sat, 15 Jan 2022 19:30:12: 9000000 INFO @ Sat, 15 Jan 2022 19:30:12: 13000000 INFO @ Sat, 15 Jan 2022 19:30:20: 10000000 INFO @ Sat, 15 Jan 2022 19:30:21: 18000000 INFO @ Sat, 15 Jan 2022 19:30:21: 14000000 INFO @ Sat, 15 Jan 2022 19:30:30: 19000000 INFO @ Sat, 15 Jan 2022 19:30:30: 11000000 INFO @ Sat, 15 Jan 2022 19:30:30: 15000000 INFO @ Sat, 15 Jan 2022 19:30:39: 20000000 INFO @ Sat, 15 Jan 2022 19:30:39: 12000000 INFO @ Sat, 15 Jan 2022 19:30:40: 16000000 INFO @ Sat, 15 Jan 2022 19:30:47: 21000000 INFO @ Sat, 15 Jan 2022 19:30:49: 13000000 INFO @ Sat, 15 Jan 2022 19:30:49: 17000000 INFO @ Sat, 15 Jan 2022 19:30:56: 22000000 INFO @ Sat, 15 Jan 2022 19:30:58: 14000000 INFO @ Sat, 15 Jan 2022 19:30:58: 18000000 INFO @ Sat, 15 Jan 2022 19:31:05: 23000000 INFO @ Sat, 15 Jan 2022 19:31:07: 19000000 INFO @ Sat, 15 Jan 2022 19:31:07: 15000000 INFO @ Sat, 15 Jan 2022 19:31:14: 24000000 INFO @ Sat, 15 Jan 2022 19:31:16: 20000000 INFO @ Sat, 15 Jan 2022 19:31:17: 16000000 INFO @ Sat, 15 Jan 2022 19:31:23: 25000000 INFO @ Sat, 15 Jan 2022 19:31:26: 21000000 INFO @ Sat, 15 Jan 2022 19:31:26: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:31:32: 26000000 INFO @ Sat, 15 Jan 2022 19:31:36: 22000000 INFO @ Sat, 15 Jan 2022 19:31:36: 18000000 INFO @ Sat, 15 Jan 2022 19:31:41: 27000000 INFO @ Sat, 15 Jan 2022 19:31:45: 23000000 INFO @ Sat, 15 Jan 2022 19:31:46: 19000000 INFO @ Sat, 15 Jan 2022 19:31:50: 28000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:31:55: 24000000 INFO @ Sat, 15 Jan 2022 19:31:56: 20000000 INFO @ Sat, 15 Jan 2022 19:31:58: 29000000 INFO @ Sat, 15 Jan 2022 19:32:04: 25000000 INFO @ Sat, 15 Jan 2022 19:32:06: 21000000 INFO @ Sat, 15 Jan 2022 19:32:07: 30000000 INFO @ Sat, 15 Jan 2022 19:32:13: 26000000 INFO @ Sat, 15 Jan 2022 19:32:15: 31000000 INFO @ Sat, 15 Jan 2022 19:32:16: 22000000 INFO @ Sat, 15 Jan 2022 19:32:22: 27000000 INFO @ Sat, 15 Jan 2022 19:32:24: 32000000 INFO @ Sat, 15 Jan 2022 19:32:26: 23000000 INFO @ Sat, 15 Jan 2022 19:32:32: 28000000 INFO @ Sat, 15 Jan 2022 19:32:33: 33000000 INFO @ Sat, 15 Jan 2022 19:32:35: 24000000 INFO @ Sat, 15 Jan 2022 19:32:41: 29000000 INFO @ Sat, 15 Jan 2022 19:32:42: 34000000 INFO @ Sat, 15 Jan 2022 19:32:45: 25000000 INFO @ Sat, 15 Jan 2022 19:32:50: 35000000 INFO @ Sat, 15 Jan 2022 19:32:51: 30000000 INFO @ Sat, 15 Jan 2022 19:32:54: 26000000 INFO @ Sat, 15 Jan 2022 19:32:59: 36000000 INFO @ Sat, 15 Jan 2022 19:33:00: 31000000 INFO @ Sat, 15 Jan 2022 19:33:04: 27000000 INFO @ Sat, 15 Jan 2022 19:33:08: 37000000 INFO @ Sat, 15 Jan 2022 19:33:10: 32000000 INFO @ Sat, 15 Jan 2022 19:33:13: 28000000 INFO @ Sat, 15 Jan 2022 19:33:17: 38000000 INFO @ Sat, 15 Jan 2022 19:33:20: 33000000 INFO @ Sat, 15 Jan 2022 19:33:22: 29000000 INFO @ Sat, 15 Jan 2022 19:33:26: 39000000 INFO @ Sat, 15 Jan 2022 19:33:29: 34000000 INFO @ Sat, 15 Jan 2022 19:33:32: 30000000 INFO @ Sat, 15 Jan 2022 19:33:35: 40000000 INFO @ Sat, 15 Jan 2022 19:33:39: 35000000 INFO @ Sat, 15 Jan 2022 19:33:41: 31000000 INFO @ Sat, 15 Jan 2022 19:33:43: 41000000 INFO @ Sat, 15 Jan 2022 19:33:49: 36000000 INFO @ Sat, 15 Jan 2022 19:33:51: 32000000 INFO @ Sat, 15 Jan 2022 19:33:52: 42000000 INFO @ Sat, 15 Jan 2022 19:33:58: 37000000 INFO @ Sat, 15 Jan 2022 19:34:00: 33000000 INFO @ Sat, 15 Jan 2022 19:34:00: 43000000 INFO @ Sat, 15 Jan 2022 19:34:07: 38000000 INFO @ Sat, 15 Jan 2022 19:34:10: 44000000 INFO @ Sat, 15 Jan 2022 19:34:10: 34000000 INFO @ Sat, 15 Jan 2022 19:34:16: 39000000 INFO @ Sat, 15 Jan 2022 19:34:18: 45000000 INFO @ Sat, 15 Jan 2022 19:34:20: 35000000 INFO @ Sat, 15 Jan 2022 19:34:25: 40000000 INFO @ Sat, 15 Jan 2022 19:34:27: 46000000 INFO @ Sat, 15 Jan 2022 19:34:28: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:34:28: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:34:28: #1 total tags in treatment: 22321304 INFO @ Sat, 15 Jan 2022 19:34:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:34:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:34:29: #1 tags after filtering in treatment: 8035459 INFO @ Sat, 15 Jan 2022 19:34:29: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 15 Jan 2022 19:34:29: #1 finished! INFO @ Sat, 15 Jan 2022 19:34:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:34:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:34:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:34:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:34:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Sat, 15 Jan 2022 19:34:30: 36000000 rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:34:34: 41000000 INFO @ Sat, 15 Jan 2022 19:34:39: 37000000 INFO @ Sat, 15 Jan 2022 19:34:43: 42000000 INFO @ Sat, 15 Jan 2022 19:34:48: 38000000 INFO @ Sat, 15 Jan 2022 19:34:53: 43000000 INFO @ Sat, 15 Jan 2022 19:34:57: 39000000 INFO @ Sat, 15 Jan 2022 19:35:02: 44000000 INFO @ Sat, 15 Jan 2022 19:35:06: 40000000 INFO @ Sat, 15 Jan 2022 19:35:11: 45000000 INFO @ Sat, 15 Jan 2022 19:35:16: 41000000 INFO @ Sat, 15 Jan 2022 19:35:20: 46000000 INFO @ Sat, 15 Jan 2022 19:35:21: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:35:21: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:35:21: #1 total tags in treatment: 22321304 INFO @ Sat, 15 Jan 2022 19:35:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:22: #1 tags after filtering in treatment: 8035459 INFO @ Sat, 15 Jan 2022 19:35:22: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 15 Jan 2022 19:35:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:35:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:35:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:35:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:35:25: 42000000 INFO @ Sat, 15 Jan 2022 19:35:33: 43000000 INFO @ Sat, 15 Jan 2022 19:35:41: 44000000 INFO @ Sat, 15 Jan 2022 19:35:50: 45000000 INFO @ Sat, 15 Jan 2022 19:35:57: 46000000 INFO @ Sat, 15 Jan 2022 19:35:58: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:35:58: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:35:58: #1 total tags in treatment: 22321304 INFO @ Sat, 15 Jan 2022 19:35:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:59: #1 tags after filtering in treatment: 8035459 INFO @ Sat, 15 Jan 2022 19:35:59: #1 Redundant rate of treatment: 0.64 INFO @ Sat, 15 Jan 2022 19:35:59: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829457/SRX8829457.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling