Job ID = 14520004 SRX = SRX8829453 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 45086198 spots for SRR12329247/SRR12329247.sra Written 45086198 spots for SRR12329247/SRR12329247.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:13 45086198 reads; of these: 45086198 (100.00%) were paired; of these: 2562439 (5.68%) aligned concordantly 0 times 36347186 (80.62%) aligned concordantly exactly 1 time 6176573 (13.70%) aligned concordantly >1 times ---- 2562439 pairs aligned concordantly 0 times; of these: 239174 (9.33%) aligned discordantly 1 time ---- 2323265 pairs aligned 0 times concordantly or discordantly; of these: 4646530 mates make up the pairs; of these: 4126411 (88.81%) aligned 0 times 321148 (6.91%) aligned exactly 1 time 198971 (4.28%) aligned >1 times 95.42% overall alignment rate Time searching: 00:35:13 Overall time: 00:35:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 15239150 / 42747166 = 0.3565 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:55: 1000000 INFO @ Sat, 15 Jan 2022 19:15:00: 2000000 INFO @ Sat, 15 Jan 2022 19:15:06: 3000000 INFO @ Sat, 15 Jan 2022 19:15:11: 4000000 INFO @ Sat, 15 Jan 2022 19:15:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:21: 6000000 INFO @ Sat, 15 Jan 2022 19:15:26: 1000000 INFO @ Sat, 15 Jan 2022 19:15:26: 7000000 INFO @ Sat, 15 Jan 2022 19:15:31: 2000000 INFO @ Sat, 15 Jan 2022 19:15:31: 8000000 INFO @ Sat, 15 Jan 2022 19:15:36: 3000000 INFO @ Sat, 15 Jan 2022 19:15:37: 9000000 INFO @ Sat, 15 Jan 2022 19:15:41: 4000000 INFO @ Sat, 15 Jan 2022 19:15:42: 10000000 INFO @ Sat, 15 Jan 2022 19:15:46: 5000000 INFO @ Sat, 15 Jan 2022 19:15:47: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:51: 6000000 INFO @ Sat, 15 Jan 2022 19:15:52: 12000000 INFO @ Sat, 15 Jan 2022 19:15:56: 7000000 INFO @ Sat, 15 Jan 2022 19:15:56: 1000000 INFO @ Sat, 15 Jan 2022 19:15:58: 13000000 INFO @ Sat, 15 Jan 2022 19:16:01: 8000000 INFO @ Sat, 15 Jan 2022 19:16:02: 2000000 INFO @ Sat, 15 Jan 2022 19:16:04: 14000000 INFO @ Sat, 15 Jan 2022 19:16:06: 9000000 INFO @ Sat, 15 Jan 2022 19:16:08: 3000000 INFO @ Sat, 15 Jan 2022 19:16:10: 15000000 INFO @ Sat, 15 Jan 2022 19:16:11: 10000000 INFO @ Sat, 15 Jan 2022 19:16:14: 4000000 INFO @ Sat, 15 Jan 2022 19:16:16: 16000000 INFO @ Sat, 15 Jan 2022 19:16:16: 11000000 INFO @ Sat, 15 Jan 2022 19:16:20: 5000000 INFO @ Sat, 15 Jan 2022 19:16:22: 12000000 INFO @ Sat, 15 Jan 2022 19:16:22: 17000000 INFO @ Sat, 15 Jan 2022 19:16:26: 6000000 INFO @ Sat, 15 Jan 2022 19:16:27: 13000000 INFO @ Sat, 15 Jan 2022 19:16:28: 18000000 INFO @ Sat, 15 Jan 2022 19:16:31: 7000000 INFO @ Sat, 15 Jan 2022 19:16:32: 14000000 INFO @ Sat, 15 Jan 2022 19:16:34: 19000000 INFO @ Sat, 15 Jan 2022 19:16:37: 8000000 INFO @ Sat, 15 Jan 2022 19:16:37: 15000000 INFO @ Sat, 15 Jan 2022 19:16:40: 20000000 INFO @ Sat, 15 Jan 2022 19:16:43: 16000000 INFO @ Sat, 15 Jan 2022 19:16:43: 9000000 INFO @ Sat, 15 Jan 2022 19:16:46: 21000000 INFO @ Sat, 15 Jan 2022 19:16:48: 17000000 INFO @ Sat, 15 Jan 2022 19:16:49: 10000000 INFO @ Sat, 15 Jan 2022 19:16:52: 22000000 INFO @ Sat, 15 Jan 2022 19:16:53: 18000000 INFO @ Sat, 15 Jan 2022 19:16:55: 11000000 INFO @ Sat, 15 Jan 2022 19:16:58: 19000000 INFO @ Sat, 15 Jan 2022 19:16:58: 23000000 INFO @ Sat, 15 Jan 2022 19:17:01: 12000000 INFO @ Sat, 15 Jan 2022 19:17:03: 20000000 INFO @ Sat, 15 Jan 2022 19:17:04: 24000000 INFO @ Sat, 15 Jan 2022 19:17:07: 13000000 INFO @ Sat, 15 Jan 2022 19:17:08: 21000000 INFO @ Sat, 15 Jan 2022 19:17:10: 25000000 INFO @ Sat, 15 Jan 2022 19:17:13: 14000000 INFO @ Sat, 15 Jan 2022 19:17:13: 22000000 INFO @ Sat, 15 Jan 2022 19:17:16: 26000000 INFO @ Sat, 15 Jan 2022 19:17:18: 23000000 INFO @ Sat, 15 Jan 2022 19:17:19: 15000000 INFO @ Sat, 15 Jan 2022 19:17:21: 27000000 INFO @ Sat, 15 Jan 2022 19:17:23: 24000000 INFO @ Sat, 15 Jan 2022 19:17:25: 16000000 INFO @ Sat, 15 Jan 2022 19:17:27: 28000000 INFO @ Sat, 15 Jan 2022 19:17:28: 25000000 INFO @ Sat, 15 Jan 2022 19:17:31: 17000000 INFO @ Sat, 15 Jan 2022 19:17:33: 26000000 INFO @ Sat, 15 Jan 2022 19:17:33: 29000000 INFO @ Sat, 15 Jan 2022 19:17:37: 18000000 INFO @ Sat, 15 Jan 2022 19:17:38: 27000000 INFO @ Sat, 15 Jan 2022 19:17:40: 30000000 INFO @ Sat, 15 Jan 2022 19:17:43: 19000000 INFO @ Sat, 15 Jan 2022 19:17:43: 28000000 INFO @ Sat, 15 Jan 2022 19:17:46: 31000000 INFO @ Sat, 15 Jan 2022 19:17:48: 29000000 INFO @ Sat, 15 Jan 2022 19:17:49: 20000000 INFO @ Sat, 15 Jan 2022 19:17:51: 32000000 INFO @ Sat, 15 Jan 2022 19:17:53: 30000000 INFO @ Sat, 15 Jan 2022 19:17:54: 21000000 INFO @ Sat, 15 Jan 2022 19:17:57: 33000000 INFO @ Sat, 15 Jan 2022 19:17:58: 31000000 INFO @ Sat, 15 Jan 2022 19:18:00: 22000000 INFO @ Sat, 15 Jan 2022 19:18:03: 34000000 INFO @ Sat, 15 Jan 2022 19:18:03: 32000000 INFO @ Sat, 15 Jan 2022 19:18:06: 23000000 INFO @ Sat, 15 Jan 2022 19:18:08: 33000000 INFO @ Sat, 15 Jan 2022 19:18:09: 35000000 INFO @ Sat, 15 Jan 2022 19:18:12: 24000000 INFO @ Sat, 15 Jan 2022 19:18:13: 34000000 INFO @ Sat, 15 Jan 2022 19:18:15: 36000000 INFO @ Sat, 15 Jan 2022 19:18:18: 25000000 INFO @ Sat, 15 Jan 2022 19:18:18: 35000000 INFO @ Sat, 15 Jan 2022 19:18:21: 37000000 INFO @ Sat, 15 Jan 2022 19:18:24: 36000000 INFO @ Sat, 15 Jan 2022 19:18:24: 26000000 INFO @ Sat, 15 Jan 2022 19:18:27: 38000000 INFO @ Sat, 15 Jan 2022 19:18:29: 37000000 INFO @ Sat, 15 Jan 2022 19:18:30: 27000000 INFO @ Sat, 15 Jan 2022 19:18:33: 39000000 INFO @ Sat, 15 Jan 2022 19:18:34: 38000000 INFO @ Sat, 15 Jan 2022 19:18:36: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:18:39: 39000000 INFO @ Sat, 15 Jan 2022 19:18:39: 40000000 INFO @ Sat, 15 Jan 2022 19:18:42: 29000000 INFO @ Sat, 15 Jan 2022 19:18:44: 40000000 INFO @ Sat, 15 Jan 2022 19:18:45: 41000000 INFO @ Sat, 15 Jan 2022 19:18:48: 30000000 INFO @ Sat, 15 Jan 2022 19:18:49: 41000000 INFO @ Sat, 15 Jan 2022 19:18:51: 42000000 INFO @ Sat, 15 Jan 2022 19:18:53: 31000000 INFO @ Sat, 15 Jan 2022 19:18:54: 42000000 INFO @ Sat, 15 Jan 2022 19:18:57: 43000000 INFO @ Sat, 15 Jan 2022 19:18:59: 43000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:18:59: 32000000 INFO @ Sat, 15 Jan 2022 19:19:03: 44000000 INFO @ Sat, 15 Jan 2022 19:19:04: 44000000 INFO @ Sat, 15 Jan 2022 19:19:05: 33000000 INFO @ Sat, 15 Jan 2022 19:19:09: 45000000 INFO @ Sat, 15 Jan 2022 19:19:09: 45000000 INFO @ Sat, 15 Jan 2022 19:19:11: 34000000 INFO @ Sat, 15 Jan 2022 19:19:14: 46000000 INFO @ Sat, 15 Jan 2022 19:19:15: 46000000 INFO @ Sat, 15 Jan 2022 19:19:17: 35000000 INFO @ Sat, 15 Jan 2022 19:19:19: 47000000 INFO @ Sat, 15 Jan 2022 19:19:21: 47000000 INFO @ Sat, 15 Jan 2022 19:19:23: 36000000 INFO @ Sat, 15 Jan 2022 19:19:24: 48000000 INFO @ Sat, 15 Jan 2022 19:19:26: 48000000 INFO @ Sat, 15 Jan 2022 19:19:29: 49000000 INFO @ Sat, 15 Jan 2022 19:19:29: 37000000 INFO @ Sat, 15 Jan 2022 19:19:32: 49000000 INFO @ Sat, 15 Jan 2022 19:19:34: 50000000 INFO @ Sat, 15 Jan 2022 19:19:35: 38000000 INFO @ Sat, 15 Jan 2022 19:19:39: 50000000 INFO @ Sat, 15 Jan 2022 19:19:40: 51000000 INFO @ Sat, 15 Jan 2022 19:19:41: 39000000 INFO @ Sat, 15 Jan 2022 19:19:45: 52000000 INFO @ Sat, 15 Jan 2022 19:19:45: 51000000 INFO @ Sat, 15 Jan 2022 19:19:47: 40000000 INFO @ Sat, 15 Jan 2022 19:19:50: 53000000 INFO @ Sat, 15 Jan 2022 19:19:51: 52000000 INFO @ Sat, 15 Jan 2022 19:19:53: 41000000 INFO @ Sat, 15 Jan 2022 19:19:55: 54000000 INFO @ Sat, 15 Jan 2022 19:19:57: 53000000 INFO @ Sat, 15 Jan 2022 19:19:59: 42000000 INFO @ Sat, 15 Jan 2022 19:20:00: 55000000 INFO @ Sat, 15 Jan 2022 19:20:03: 54000000 INFO @ Sat, 15 Jan 2022 19:20:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:20:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:20:03: #1 total tags in treatment: 27303507 INFO @ Sat, 15 Jan 2022 19:20:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:04: #1 tags after filtering in treatment: 8656532 INFO @ Sat, 15 Jan 2022 19:20:04: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:20:04: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:20:05: 43000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:20:09: 55000000 INFO @ Sat, 15 Jan 2022 19:20:11: 44000000 INFO @ Sat, 15 Jan 2022 19:20:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:20:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:20:12: #1 total tags in treatment: 27303507 INFO @ Sat, 15 Jan 2022 19:20:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:13: #1 tags after filtering in treatment: 8656532 INFO @ Sat, 15 Jan 2022 19:20:13: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:20:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:20:16: 45000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:20:21: 46000000 INFO @ Sat, 15 Jan 2022 19:20:26: 47000000 INFO @ Sat, 15 Jan 2022 19:20:31: 48000000 INFO @ Sat, 15 Jan 2022 19:20:36: 49000000 INFO @ Sat, 15 Jan 2022 19:20:41: 50000000 INFO @ Sat, 15 Jan 2022 19:20:46: 51000000 INFO @ Sat, 15 Jan 2022 19:20:51: 52000000 INFO @ Sat, 15 Jan 2022 19:20:56: 53000000 INFO @ Sat, 15 Jan 2022 19:21:01: 54000000 INFO @ Sat, 15 Jan 2022 19:21:06: 55000000 INFO @ Sat, 15 Jan 2022 19:21:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:21:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:21:10: #1 total tags in treatment: 27303507 INFO @ Sat, 15 Jan 2022 19:21:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:10: #1 tags after filtering in treatment: 8656532 INFO @ Sat, 15 Jan 2022 19:21:10: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:21:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:21:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829453/SRX8829453.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling