Job ID = 14520003 SRX = SRX8829452 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 39836914 spots for SRR12329246/SRR12329246.sra Written 39836914 spots for SRR12329246/SRR12329246.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:10 39836914 reads; of these: 39836914 (100.00%) were paired; of these: 2223382 (5.58%) aligned concordantly 0 times 29727459 (74.62%) aligned concordantly exactly 1 time 7886073 (19.80%) aligned concordantly >1 times ---- 2223382 pairs aligned concordantly 0 times; of these: 454183 (20.43%) aligned discordantly 1 time ---- 1769199 pairs aligned 0 times concordantly or discordantly; of these: 3538398 mates make up the pairs; of these: 2784269 (78.69%) aligned 0 times 438159 (12.38%) aligned exactly 1 time 315970 (8.93%) aligned >1 times 96.51% overall alignment rate Time searching: 00:37:10 Overall time: 00:37:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 11302869 / 38048958 = 0.2971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:59: 1000000 INFO @ Sat, 15 Jan 2022 19:16:08: 2000000 INFO @ Sat, 15 Jan 2022 19:16:16: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:16:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:16:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:16:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:16:24: 4000000 INFO @ Sat, 15 Jan 2022 19:16:30: 1000000 INFO @ Sat, 15 Jan 2022 19:16:33: 5000000 INFO @ Sat, 15 Jan 2022 19:16:39: 2000000 INFO @ Sat, 15 Jan 2022 19:16:41: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:16:48: 3000000 INFO @ Sat, 15 Jan 2022 19:16:49: 7000000 INFO @ Sat, 15 Jan 2022 19:16:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:16:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:16:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:16:57: 4000000 INFO @ Sat, 15 Jan 2022 19:16:58: 8000000 INFO @ Sat, 15 Jan 2022 19:17:00: 1000000 INFO @ Sat, 15 Jan 2022 19:17:07: 5000000 INFO @ Sat, 15 Jan 2022 19:17:07: 9000000 INFO @ Sat, 15 Jan 2022 19:17:09: 2000000 INFO @ Sat, 15 Jan 2022 19:17:16: 6000000 INFO @ Sat, 15 Jan 2022 19:17:16: 10000000 INFO @ Sat, 15 Jan 2022 19:17:18: 3000000 INFO @ Sat, 15 Jan 2022 19:17:25: 11000000 INFO @ Sat, 15 Jan 2022 19:17:26: 7000000 INFO @ Sat, 15 Jan 2022 19:17:27: 4000000 INFO @ Sat, 15 Jan 2022 19:17:33: 12000000 INFO @ Sat, 15 Jan 2022 19:17:36: 5000000 INFO @ Sat, 15 Jan 2022 19:17:36: 8000000 INFO @ Sat, 15 Jan 2022 19:17:42: 13000000 INFO @ Sat, 15 Jan 2022 19:17:44: 6000000 INFO @ Sat, 15 Jan 2022 19:17:46: 9000000 INFO @ Sat, 15 Jan 2022 19:17:51: 14000000 INFO @ Sat, 15 Jan 2022 19:17:53: 7000000 INFO @ Sat, 15 Jan 2022 19:17:55: 10000000 INFO @ Sat, 15 Jan 2022 19:18:00: 15000000 INFO @ Sat, 15 Jan 2022 19:18:02: 8000000 INFO @ Sat, 15 Jan 2022 19:18:05: 11000000 INFO @ Sat, 15 Jan 2022 19:18:08: 16000000 INFO @ Sat, 15 Jan 2022 19:18:11: 9000000 INFO @ Sat, 15 Jan 2022 19:18:14: 12000000 INFO @ Sat, 15 Jan 2022 19:18:17: 17000000 INFO @ Sat, 15 Jan 2022 19:18:19: 10000000 INFO @ Sat, 15 Jan 2022 19:18:24: 13000000 INFO @ Sat, 15 Jan 2022 19:18:26: 18000000 INFO @ Sat, 15 Jan 2022 19:18:28: 11000000 INFO @ Sat, 15 Jan 2022 19:18:34: 14000000 INFO @ Sat, 15 Jan 2022 19:18:35: 19000000 INFO @ Sat, 15 Jan 2022 19:18:37: 12000000 INFO @ Sat, 15 Jan 2022 19:18:43: 15000000 INFO @ Sat, 15 Jan 2022 19:18:44: 20000000 INFO @ Sat, 15 Jan 2022 19:18:46: 13000000 INFO @ Sat, 15 Jan 2022 19:18:52: 21000000 INFO @ Sat, 15 Jan 2022 19:18:53: 16000000 INFO @ Sat, 15 Jan 2022 19:18:55: 14000000 INFO @ Sat, 15 Jan 2022 19:19:01: 22000000 INFO @ Sat, 15 Jan 2022 19:19:03: 17000000 INFO @ Sat, 15 Jan 2022 19:19:03: 15000000 INFO @ Sat, 15 Jan 2022 19:19:10: 23000000 INFO @ Sat, 15 Jan 2022 19:19:12: 16000000 INFO @ Sat, 15 Jan 2022 19:19:13: 18000000 INFO @ Sat, 15 Jan 2022 19:19:19: 24000000 INFO @ Sat, 15 Jan 2022 19:19:21: 17000000 INFO @ Sat, 15 Jan 2022 19:19:22: 19000000 INFO @ Sat, 15 Jan 2022 19:19:28: 25000000 INFO @ Sat, 15 Jan 2022 19:19:30: 18000000 INFO @ Sat, 15 Jan 2022 19:19:31: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:19:37: 26000000 INFO @ Sat, 15 Jan 2022 19:19:38: 19000000 INFO @ Sat, 15 Jan 2022 19:19:41: 21000000 INFO @ Sat, 15 Jan 2022 19:19:45: 27000000 INFO @ Sat, 15 Jan 2022 19:19:47: 20000000 INFO @ Sat, 15 Jan 2022 19:19:50: 22000000 INFO @ Sat, 15 Jan 2022 19:19:54: 28000000 INFO @ Sat, 15 Jan 2022 19:19:56: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:19:59: 23000000 INFO @ Sat, 15 Jan 2022 19:20:03: 29000000 INFO @ Sat, 15 Jan 2022 19:20:05: 22000000 INFO @ Sat, 15 Jan 2022 19:20:08: 24000000 INFO @ Sat, 15 Jan 2022 19:20:12: 30000000 INFO @ Sat, 15 Jan 2022 19:20:14: 23000000 INFO @ Sat, 15 Jan 2022 19:20:17: 25000000 INFO @ Sat, 15 Jan 2022 19:20:21: 31000000 INFO @ Sat, 15 Jan 2022 19:20:23: 24000000 INFO @ Sat, 15 Jan 2022 19:20:27: 26000000 INFO @ Sat, 15 Jan 2022 19:20:30: 32000000 INFO @ Sat, 15 Jan 2022 19:20:31: 25000000 INFO @ Sat, 15 Jan 2022 19:20:36: 27000000 INFO @ Sat, 15 Jan 2022 19:20:38: 33000000 INFO @ Sat, 15 Jan 2022 19:20:40: 26000000 INFO @ Sat, 15 Jan 2022 19:20:45: 28000000 INFO @ Sat, 15 Jan 2022 19:20:47: 34000000 INFO @ Sat, 15 Jan 2022 19:20:49: 27000000 INFO @ Sat, 15 Jan 2022 19:20:55: 29000000 INFO @ Sat, 15 Jan 2022 19:20:56: 35000000 INFO @ Sat, 15 Jan 2022 19:20:57: 28000000 INFO @ Sat, 15 Jan 2022 19:21:04: 30000000 INFO @ Sat, 15 Jan 2022 19:21:05: 36000000 INFO @ Sat, 15 Jan 2022 19:21:06: 29000000 INFO @ Sat, 15 Jan 2022 19:21:13: 31000000 INFO @ Sat, 15 Jan 2022 19:21:13: 37000000 INFO @ Sat, 15 Jan 2022 19:21:15: 30000000 INFO @ Sat, 15 Jan 2022 19:21:22: 38000000 INFO @ Sat, 15 Jan 2022 19:21:22: 32000000 INFO @ Sat, 15 Jan 2022 19:21:24: 31000000 INFO @ Sat, 15 Jan 2022 19:21:31: 39000000 INFO @ Sat, 15 Jan 2022 19:21:32: 33000000 INFO @ Sat, 15 Jan 2022 19:21:32: 32000000 INFO @ Sat, 15 Jan 2022 19:21:40: 40000000 INFO @ Sat, 15 Jan 2022 19:21:41: 34000000 INFO @ Sat, 15 Jan 2022 19:21:41: 33000000 INFO @ Sat, 15 Jan 2022 19:21:48: 41000000 INFO @ Sat, 15 Jan 2022 19:21:50: 34000000 INFO @ Sat, 15 Jan 2022 19:21:50: 35000000 INFO @ Sat, 15 Jan 2022 19:21:57: 42000000 INFO @ Sat, 15 Jan 2022 19:21:59: 35000000 INFO @ Sat, 15 Jan 2022 19:22:00: 36000000 INFO @ Sat, 15 Jan 2022 19:22:06: 43000000 INFO @ Sat, 15 Jan 2022 19:22:07: 36000000 INFO @ Sat, 15 Jan 2022 19:22:09: 37000000 INFO @ Sat, 15 Jan 2022 19:22:14: 44000000 INFO @ Sat, 15 Jan 2022 19:22:16: 37000000 INFO @ Sat, 15 Jan 2022 19:22:19: 38000000 INFO @ Sat, 15 Jan 2022 19:22:23: 45000000 INFO @ Sat, 15 Jan 2022 19:22:25: 38000000 INFO @ Sat, 15 Jan 2022 19:22:28: 39000000 INFO @ Sat, 15 Jan 2022 19:22:32: 46000000 INFO @ Sat, 15 Jan 2022 19:22:33: 39000000 INFO @ Sat, 15 Jan 2022 19:22:37: 40000000 INFO @ Sat, 15 Jan 2022 19:22:40: 47000000 INFO @ Sat, 15 Jan 2022 19:22:42: 40000000 INFO @ Sat, 15 Jan 2022 19:22:46: 41000000 INFO @ Sat, 15 Jan 2022 19:22:49: 48000000 INFO @ Sat, 15 Jan 2022 19:22:51: 41000000 INFO @ Sat, 15 Jan 2022 19:22:55: 42000000 INFO @ Sat, 15 Jan 2022 19:22:58: 49000000 INFO @ Sat, 15 Jan 2022 19:22:59: 42000000 INFO @ Sat, 15 Jan 2022 19:23:04: 43000000 INFO @ Sat, 15 Jan 2022 19:23:08: 43000000 INFO @ Sat, 15 Jan 2022 19:23:08: 50000000 INFO @ Sat, 15 Jan 2022 19:23:13: 44000000 INFO @ Sat, 15 Jan 2022 19:23:16: 44000000 INFO @ Sat, 15 Jan 2022 19:23:16: 51000000 INFO @ Sat, 15 Jan 2022 19:23:23: 45000000 INFO @ Sat, 15 Jan 2022 19:23:25: 45000000 INFO @ Sat, 15 Jan 2022 19:23:25: 52000000 INFO @ Sat, 15 Jan 2022 19:23:32: 46000000 INFO @ Sat, 15 Jan 2022 19:23:33: 46000000 INFO @ Sat, 15 Jan 2022 19:23:33: 53000000 INFO @ Sat, 15 Jan 2022 19:23:41: 47000000 INFO @ Sat, 15 Jan 2022 19:23:42: 47000000 INFO @ Sat, 15 Jan 2022 19:23:42: 54000000 INFO @ Sat, 15 Jan 2022 19:23:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:23:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:23:45: #1 total tags in treatment: 26342319 INFO @ Sat, 15 Jan 2022 19:23:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:23:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:23:46: #1 tags after filtering in treatment: 8607565 INFO @ Sat, 15 Jan 2022 19:23:46: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:23:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:23:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:23:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:23:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:23:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:23:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:23:50: 48000000 INFO @ Sat, 15 Jan 2022 19:23:51: 48000000 INFO @ Sat, 15 Jan 2022 19:23:59: 49000000 INFO @ Sat, 15 Jan 2022 19:24:00: 49000000 INFO @ Sat, 15 Jan 2022 19:24:08: 50000000 INFO @ Sat, 15 Jan 2022 19:24:09: 50000000 INFO @ Sat, 15 Jan 2022 19:24:17: 51000000 INFO @ Sat, 15 Jan 2022 19:24:18: 51000000 INFO @ Sat, 15 Jan 2022 19:24:26: 52000000 INFO @ Sat, 15 Jan 2022 19:24:27: 52000000 INFO @ Sat, 15 Jan 2022 19:24:34: 53000000 INFO @ Sat, 15 Jan 2022 19:24:36: 53000000 INFO @ Sat, 15 Jan 2022 19:24:43: 54000000 INFO @ Sat, 15 Jan 2022 19:24:45: 54000000 INFO @ Sat, 15 Jan 2022 19:24:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:24:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:24:45: #1 total tags in treatment: 26342319 INFO @ Sat, 15 Jan 2022 19:24:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:24:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:24:46: #1 tags after filtering in treatment: 8607565 INFO @ Sat, 15 Jan 2022 19:24:46: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:24:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:24:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:24:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:24:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:24:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:24:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:24:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:24:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:24:48: #1 total tags in treatment: 26342319 INFO @ Sat, 15 Jan 2022 19:24:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:24:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:24:49: #1 tags after filtering in treatment: 8607565 INFO @ Sat, 15 Jan 2022 19:24:49: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:24:49: #1 finished! INFO @ Sat, 15 Jan 2022 19:24:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:24:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:24:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:24:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:24:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829452/SRX8829452.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling