Job ID = 14520001 SRX = SRX8829450 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 42879530 spots for SRR12329244/SRR12329244.sra Written 42879530 spots for SRR12329244/SRR12329244.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:27 42879530 reads; of these: 42879530 (100.00%) were paired; of these: 3048274 (7.11%) aligned concordantly 0 times 30613527 (71.39%) aligned concordantly exactly 1 time 9217729 (21.50%) aligned concordantly >1 times ---- 3048274 pairs aligned concordantly 0 times; of these: 449180 (14.74%) aligned discordantly 1 time ---- 2599094 pairs aligned 0 times concordantly or discordantly; of these: 5198188 mates make up the pairs; of these: 4306585 (82.85%) aligned 0 times 469252 (9.03%) aligned exactly 1 time 422351 (8.12%) aligned >1 times 94.98% overall alignment rate Time searching: 00:26:28 Overall time: 00:26:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12726833 / 40266216 = 0.3161 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:24:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:24:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:24:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:24:40: 1000000 INFO @ Sat, 15 Jan 2022 19:24:45: 2000000 INFO @ Sat, 15 Jan 2022 19:24:50: 3000000 INFO @ Sat, 15 Jan 2022 19:24:56: 4000000 INFO @ Sat, 15 Jan 2022 19:25:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:25:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:25:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:25:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:25:07: 6000000 INFO @ Sat, 15 Jan 2022 19:25:10: 1000000 INFO @ Sat, 15 Jan 2022 19:25:13: 7000000 INFO @ Sat, 15 Jan 2022 19:25:16: 2000000 INFO @ Sat, 15 Jan 2022 19:25:19: 8000000 INFO @ Sat, 15 Jan 2022 19:25:22: 3000000 INFO @ Sat, 15 Jan 2022 19:25:24: 9000000 INFO @ Sat, 15 Jan 2022 19:25:28: 4000000 INFO @ Sat, 15 Jan 2022 19:25:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:25:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:25:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:25:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:25:35: 5000000 INFO @ Sat, 15 Jan 2022 19:25:36: 11000000 INFO @ Sat, 15 Jan 2022 19:25:40: 1000000 INFO @ Sat, 15 Jan 2022 19:25:41: 6000000 INFO @ Sat, 15 Jan 2022 19:25:42: 12000000 INFO @ Sat, 15 Jan 2022 19:25:47: 2000000 INFO @ Sat, 15 Jan 2022 19:25:47: 7000000 INFO @ Sat, 15 Jan 2022 19:25:49: 13000000 INFO @ Sat, 15 Jan 2022 19:25:53: 3000000 INFO @ Sat, 15 Jan 2022 19:25:54: 8000000 INFO @ Sat, 15 Jan 2022 19:25:55: 14000000 INFO @ Sat, 15 Jan 2022 19:25:59: 4000000 INFO @ Sat, 15 Jan 2022 19:26:00: 9000000 INFO @ Sat, 15 Jan 2022 19:26:02: 15000000 INFO @ Sat, 15 Jan 2022 19:26:05: 5000000 INFO @ Sat, 15 Jan 2022 19:26:07: 10000000 INFO @ Sat, 15 Jan 2022 19:26:08: 16000000 INFO @ Sat, 15 Jan 2022 19:26:12: 6000000 INFO @ Sat, 15 Jan 2022 19:26:13: 11000000 INFO @ Sat, 15 Jan 2022 19:26:14: 17000000 INFO @ Sat, 15 Jan 2022 19:26:18: 7000000 INFO @ Sat, 15 Jan 2022 19:26:19: 12000000 INFO @ Sat, 15 Jan 2022 19:26:20: 18000000 INFO @ Sat, 15 Jan 2022 19:26:25: 8000000 INFO @ Sat, 15 Jan 2022 19:26:26: 13000000 INFO @ Sat, 15 Jan 2022 19:26:26: 19000000 INFO @ Sat, 15 Jan 2022 19:26:31: 9000000 INFO @ Sat, 15 Jan 2022 19:26:32: 20000000 INFO @ Sat, 15 Jan 2022 19:26:32: 14000000 INFO @ Sat, 15 Jan 2022 19:26:37: 10000000 INFO @ Sat, 15 Jan 2022 19:26:37: 21000000 INFO @ Sat, 15 Jan 2022 19:26:38: 15000000 INFO @ Sat, 15 Jan 2022 19:26:43: 22000000 INFO @ Sat, 15 Jan 2022 19:26:44: 11000000 INFO @ Sat, 15 Jan 2022 19:26:45: 16000000 INFO @ Sat, 15 Jan 2022 19:26:49: 23000000 INFO @ Sat, 15 Jan 2022 19:26:50: 12000000 INFO @ Sat, 15 Jan 2022 19:26:51: 17000000 INFO @ Sat, 15 Jan 2022 19:26:55: 24000000 INFO @ Sat, 15 Jan 2022 19:26:56: 13000000 INFO @ Sat, 15 Jan 2022 19:26:57: 18000000 INFO @ Sat, 15 Jan 2022 19:27:01: 25000000 INFO @ Sat, 15 Jan 2022 19:27:03: 14000000 INFO @ Sat, 15 Jan 2022 19:27:03: 19000000 INFO @ Sat, 15 Jan 2022 19:27:06: 26000000 INFO @ Sat, 15 Jan 2022 19:27:09: 15000000 INFO @ Sat, 15 Jan 2022 19:27:09: 20000000 INFO @ Sat, 15 Jan 2022 19:27:12: 27000000 INFO @ Sat, 15 Jan 2022 19:27:15: 16000000 INFO @ Sat, 15 Jan 2022 19:27:15: 21000000 INFO @ Sat, 15 Jan 2022 19:27:18: 28000000 INFO @ Sat, 15 Jan 2022 19:27:21: 22000000 INFO @ Sat, 15 Jan 2022 19:27:21: 17000000 INFO @ Sat, 15 Jan 2022 19:27:24: 29000000 INFO @ Sat, 15 Jan 2022 19:27:27: 23000000 INFO @ Sat, 15 Jan 2022 19:27:27: 18000000 INFO @ Sat, 15 Jan 2022 19:27:30: 30000000 INFO @ Sat, 15 Jan 2022 19:27:33: 24000000 INFO @ Sat, 15 Jan 2022 19:27:33: 19000000 INFO @ Sat, 15 Jan 2022 19:27:36: 31000000 INFO @ Sat, 15 Jan 2022 19:27:38: 25000000 INFO @ Sat, 15 Jan 2022 19:27:39: 20000000 INFO @ Sat, 15 Jan 2022 19:27:42: 32000000 INFO @ Sat, 15 Jan 2022 19:27:44: 26000000 INFO @ Sat, 15 Jan 2022 19:27:45: 21000000 INFO @ Sat, 15 Jan 2022 19:27:48: 33000000 INFO @ Sat, 15 Jan 2022 19:27:50: 27000000 INFO @ Sat, 15 Jan 2022 19:27:51: 22000000 INFO @ Sat, 15 Jan 2022 19:27:54: 34000000 INFO @ Sat, 15 Jan 2022 19:27:55: 28000000 INFO @ Sat, 15 Jan 2022 19:27:57: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:28:01: 29000000 INFO @ Sat, 15 Jan 2022 19:28:01: 35000000 INFO @ Sat, 15 Jan 2022 19:28:02: 24000000 INFO @ Sat, 15 Jan 2022 19:28:06: 30000000 INFO @ Sat, 15 Jan 2022 19:28:07: 36000000 INFO @ Sat, 15 Jan 2022 19:28:08: 25000000 INFO @ Sat, 15 Jan 2022 19:28:12: 31000000 INFO @ Sat, 15 Jan 2022 19:28:13: 37000000 INFO @ Sat, 15 Jan 2022 19:28:14: 26000000 INFO @ Sat, 15 Jan 2022 19:28:17: 32000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:28:19: 27000000 INFO @ Sat, 15 Jan 2022 19:28:20: 38000000 INFO @ Sat, 15 Jan 2022 19:28:23: 33000000 INFO @ Sat, 15 Jan 2022 19:28:25: 28000000 INFO @ Sat, 15 Jan 2022 19:28:26: 39000000 INFO @ Sat, 15 Jan 2022 19:28:29: 34000000 INFO @ Sat, 15 Jan 2022 19:28:31: 29000000 INFO @ Sat, 15 Jan 2022 19:28:32: 40000000 INFO @ Sat, 15 Jan 2022 19:28:34: 35000000 INFO @ Sat, 15 Jan 2022 19:28:37: 30000000 INFO @ Sat, 15 Jan 2022 19:28:39: 41000000 INFO @ Sat, 15 Jan 2022 19:28:40: 36000000 INFO @ Sat, 15 Jan 2022 19:28:42: 31000000 INFO @ Sat, 15 Jan 2022 19:28:45: 42000000 INFO @ Sat, 15 Jan 2022 19:28:45: 37000000 INFO @ Sat, 15 Jan 2022 19:28:48: 32000000 INFO @ Sat, 15 Jan 2022 19:28:51: 38000000 INFO @ Sat, 15 Jan 2022 19:28:51: 43000000 INFO @ Sat, 15 Jan 2022 19:28:53: 33000000 INFO @ Sat, 15 Jan 2022 19:28:57: 39000000 INFO @ Sat, 15 Jan 2022 19:28:58: 44000000 INFO @ Sat, 15 Jan 2022 19:28:59: 34000000 INFO @ Sat, 15 Jan 2022 19:29:02: 40000000 INFO @ Sat, 15 Jan 2022 19:29:04: 45000000 INFO @ Sat, 15 Jan 2022 19:29:04: 35000000 INFO @ Sat, 15 Jan 2022 19:29:08: 41000000 INFO @ Sat, 15 Jan 2022 19:29:10: 36000000 INFO @ Sat, 15 Jan 2022 19:29:10: 46000000 INFO @ Sat, 15 Jan 2022 19:29:13: 42000000 INFO @ Sat, 15 Jan 2022 19:29:15: 37000000 INFO @ Sat, 15 Jan 2022 19:29:17: 47000000 INFO @ Sat, 15 Jan 2022 19:29:19: 43000000 INFO @ Sat, 15 Jan 2022 19:29:20: 38000000 INFO @ Sat, 15 Jan 2022 19:29:23: 48000000 INFO @ Sat, 15 Jan 2022 19:29:24: 44000000 INFO @ Sat, 15 Jan 2022 19:29:26: 39000000 INFO @ Sat, 15 Jan 2022 19:29:29: 49000000 INFO @ Sat, 15 Jan 2022 19:29:30: 45000000 INFO @ Sat, 15 Jan 2022 19:29:32: 40000000 INFO @ Sat, 15 Jan 2022 19:29:35: 46000000 INFO @ Sat, 15 Jan 2022 19:29:35: 50000000 INFO @ Sat, 15 Jan 2022 19:29:37: 41000000 INFO @ Sat, 15 Jan 2022 19:29:41: 47000000 INFO @ Sat, 15 Jan 2022 19:29:41: 51000000 INFO @ Sat, 15 Jan 2022 19:29:43: 42000000 INFO @ Sat, 15 Jan 2022 19:29:46: 48000000 INFO @ Sat, 15 Jan 2022 19:29:48: 52000000 INFO @ Sat, 15 Jan 2022 19:29:48: 43000000 INFO @ Sat, 15 Jan 2022 19:29:52: 49000000 INFO @ Sat, 15 Jan 2022 19:29:54: 53000000 INFO @ Sat, 15 Jan 2022 19:29:54: 44000000 INFO @ Sat, 15 Jan 2022 19:29:57: 50000000 INFO @ Sat, 15 Jan 2022 19:30:00: 45000000 INFO @ Sat, 15 Jan 2022 19:30:00: 54000000 INFO @ Sat, 15 Jan 2022 19:30:03: 51000000 INFO @ Sat, 15 Jan 2022 19:30:05: 46000000 INFO @ Sat, 15 Jan 2022 19:30:06: 55000000 INFO @ Sat, 15 Jan 2022 19:30:09: 52000000 INFO @ Sat, 15 Jan 2022 19:30:11: 47000000 INFO @ Sat, 15 Jan 2022 19:30:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:30:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:30:13: #1 total tags in treatment: 27133952 INFO @ Sat, 15 Jan 2022 19:30:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:13: #1 tags after filtering in treatment: 8630227 INFO @ Sat, 15 Jan 2022 19:30:13: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:30:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 15 Jan 2022 19:30:14: 53000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:30:16: 48000000 INFO @ Sat, 15 Jan 2022 19:30:19: 54000000 INFO @ Sat, 15 Jan 2022 19:30:22: 49000000 INFO @ Sat, 15 Jan 2022 19:30:25: 55000000 INFO @ Sat, 15 Jan 2022 19:30:27: 50000000 INFO @ Sat, 15 Jan 2022 19:30:30: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:30:30: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:30:30: #1 total tags in treatment: 27133952 INFO @ Sat, 15 Jan 2022 19:30:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:31: #1 tags after filtering in treatment: 8630227 INFO @ Sat, 15 Jan 2022 19:30:31: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:30:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:30:32: 51000000 INFO @ Sat, 15 Jan 2022 19:30:37: 52000000 INFO @ Sat, 15 Jan 2022 19:30:42: 53000000 INFO @ Sat, 15 Jan 2022 19:30:47: 54000000 INFO @ Sat, 15 Jan 2022 19:30:52: 55000000 INFO @ Sat, 15 Jan 2022 19:30:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:30:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:30:58: #1 total tags in treatment: 27133952 INFO @ Sat, 15 Jan 2022 19:30:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:58: #1 tags after filtering in treatment: 8630227 INFO @ Sat, 15 Jan 2022 19:30:58: #1 Redundant rate of treatment: 0.68 INFO @ Sat, 15 Jan 2022 19:30:58: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829450/SRX8829450.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling