Job ID = 14519982 SRX = SRX8829449 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36133253 spots for SRR12329243/SRR12329243.sra Written 36133253 spots for SRR12329243/SRR12329243.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:09 36133253 reads; of these: 36133253 (100.00%) were paired; of these: 2295085 (6.35%) aligned concordantly 0 times 25572843 (70.77%) aligned concordantly exactly 1 time 8265325 (22.87%) aligned concordantly >1 times ---- 2295085 pairs aligned concordantly 0 times; of these: 370952 (16.16%) aligned discordantly 1 time ---- 1924133 pairs aligned 0 times concordantly or discordantly; of these: 3848266 mates make up the pairs; of these: 3060850 (79.54%) aligned 0 times 407585 (10.59%) aligned exactly 1 time 379831 (9.87%) aligned >1 times 95.76% overall alignment rate Time searching: 00:22:09 Overall time: 00:22:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 10484535 / 34197877 = 0.3066 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:49:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:49:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:49:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:49:28: 1000000 INFO @ Sat, 15 Jan 2022 18:49:32: 2000000 INFO @ Sat, 15 Jan 2022 18:49:37: 3000000 INFO @ Sat, 15 Jan 2022 18:49:42: 4000000 INFO @ Sat, 15 Jan 2022 18:49:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:49:52: 6000000 INFO @ Sat, 15 Jan 2022 18:49:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:49:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:49:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:49:57: 1000000 INFO @ Sat, 15 Jan 2022 18:49:58: 7000000 INFO @ Sat, 15 Jan 2022 18:50:02: 2000000 INFO @ Sat, 15 Jan 2022 18:50:03: 8000000 INFO @ Sat, 15 Jan 2022 18:50:06: 3000000 INFO @ Sat, 15 Jan 2022 18:50:08: 9000000 INFO @ Sat, 15 Jan 2022 18:50:10: 4000000 INFO @ Sat, 15 Jan 2022 18:50:13: 10000000 INFO @ Sat, 15 Jan 2022 18:50:15: 5000000 INFO @ Sat, 15 Jan 2022 18:50:18: 11000000 INFO @ Sat, 15 Jan 2022 18:50:20: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:50:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:50:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:50:24: 12000000 INFO @ Sat, 15 Jan 2022 18:50:24: 7000000 INFO @ Sat, 15 Jan 2022 18:50:27: 1000000 INFO @ Sat, 15 Jan 2022 18:50:28: 8000000 INFO @ Sat, 15 Jan 2022 18:50:28: 13000000 INFO @ Sat, 15 Jan 2022 18:50:31: 2000000 INFO @ Sat, 15 Jan 2022 18:50:33: 9000000 INFO @ Sat, 15 Jan 2022 18:50:33: 14000000 INFO @ Sat, 15 Jan 2022 18:50:35: 3000000 INFO @ Sat, 15 Jan 2022 18:50:37: 10000000 INFO @ Sat, 15 Jan 2022 18:50:38: 15000000 INFO @ Sat, 15 Jan 2022 18:50:40: 4000000 INFO @ Sat, 15 Jan 2022 18:50:41: 11000000 INFO @ Sat, 15 Jan 2022 18:50:43: 16000000 INFO @ Sat, 15 Jan 2022 18:50:44: 5000000 INFO @ Sat, 15 Jan 2022 18:50:46: 12000000 INFO @ Sat, 15 Jan 2022 18:50:48: 17000000 INFO @ Sat, 15 Jan 2022 18:50:48: 6000000 INFO @ Sat, 15 Jan 2022 18:50:50: 13000000 INFO @ Sat, 15 Jan 2022 18:50:52: 7000000 INFO @ Sat, 15 Jan 2022 18:50:53: 18000000 INFO @ Sat, 15 Jan 2022 18:50:54: 14000000 INFO @ Sat, 15 Jan 2022 18:50:57: 8000000 INFO @ Sat, 15 Jan 2022 18:50:58: 19000000 INFO @ Sat, 15 Jan 2022 18:50:58: 15000000 INFO @ Sat, 15 Jan 2022 18:51:01: 9000000 INFO @ Sat, 15 Jan 2022 18:51:03: 20000000 INFO @ Sat, 15 Jan 2022 18:51:03: 16000000 INFO @ Sat, 15 Jan 2022 18:51:05: 10000000 INFO @ Sat, 15 Jan 2022 18:51:07: 17000000 INFO @ Sat, 15 Jan 2022 18:51:08: 21000000 INFO @ Sat, 15 Jan 2022 18:51:10: 11000000 INFO @ Sat, 15 Jan 2022 18:51:11: 18000000 INFO @ Sat, 15 Jan 2022 18:51:13: 22000000 INFO @ Sat, 15 Jan 2022 18:51:14: 12000000 INFO @ Sat, 15 Jan 2022 18:51:16: 19000000 INFO @ Sat, 15 Jan 2022 18:51:18: 23000000 INFO @ Sat, 15 Jan 2022 18:51:18: 13000000 INFO @ Sat, 15 Jan 2022 18:51:20: 20000000 INFO @ Sat, 15 Jan 2022 18:51:23: 24000000 INFO @ Sat, 15 Jan 2022 18:51:23: 14000000 INFO @ Sat, 15 Jan 2022 18:51:25: 21000000 INFO @ Sat, 15 Jan 2022 18:51:27: 15000000 INFO @ Sat, 15 Jan 2022 18:51:27: 25000000 INFO @ Sat, 15 Jan 2022 18:51:29: 22000000 INFO @ Sat, 15 Jan 2022 18:51:32: 16000000 INFO @ Sat, 15 Jan 2022 18:51:32: 26000000 INFO @ Sat, 15 Jan 2022 18:51:34: 23000000 INFO @ Sat, 15 Jan 2022 18:51:36: 17000000 INFO @ Sat, 15 Jan 2022 18:51:37: 27000000 INFO @ Sat, 15 Jan 2022 18:51:38: 24000000 INFO @ Sat, 15 Jan 2022 18:51:41: 18000000 INFO @ Sat, 15 Jan 2022 18:51:42: 28000000 INFO @ Sat, 15 Jan 2022 18:51:42: 25000000 INFO @ Sat, 15 Jan 2022 18:51:45: 19000000 INFO @ Sat, 15 Jan 2022 18:51:47: 26000000 INFO @ Sat, 15 Jan 2022 18:51:48: 29000000 INFO @ Sat, 15 Jan 2022 18:51:49: 20000000 INFO @ Sat, 15 Jan 2022 18:51:51: 27000000 INFO @ Sat, 15 Jan 2022 18:51:53: 30000000 INFO @ Sat, 15 Jan 2022 18:51:54: 21000000 INFO @ Sat, 15 Jan 2022 18:51:56: 28000000 INFO @ Sat, 15 Jan 2022 18:51:58: 31000000 INFO @ Sat, 15 Jan 2022 18:51:58: 22000000 INFO @ Sat, 15 Jan 2022 18:52:00: 29000000 INFO @ Sat, 15 Jan 2022 18:52:03: 23000000 INFO @ Sat, 15 Jan 2022 18:52:03: 32000000 INFO @ Sat, 15 Jan 2022 18:52:05: 30000000 INFO @ Sat, 15 Jan 2022 18:52:07: 24000000 INFO @ Sat, 15 Jan 2022 18:52:08: 33000000 INFO @ Sat, 15 Jan 2022 18:52:09: 31000000 INFO @ Sat, 15 Jan 2022 18:52:11: 25000000 INFO @ Sat, 15 Jan 2022 18:52:13: 32000000 INFO @ Sat, 15 Jan 2022 18:52:14: 34000000 INFO @ Sat, 15 Jan 2022 18:52:16: 26000000 INFO @ Sat, 15 Jan 2022 18:52:18: 33000000 INFO @ Sat, 15 Jan 2022 18:52:19: 35000000 INFO @ Sat, 15 Jan 2022 18:52:20: 27000000 INFO @ Sat, 15 Jan 2022 18:52:22: 34000000 INFO @ Sat, 15 Jan 2022 18:52:24: 36000000 INFO @ Sat, 15 Jan 2022 18:52:24: 28000000 INFO @ Sat, 15 Jan 2022 18:52:27: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:52:29: 29000000 INFO @ Sat, 15 Jan 2022 18:52:29: 37000000 INFO @ Sat, 15 Jan 2022 18:52:31: 36000000 INFO @ Sat, 15 Jan 2022 18:52:33: 30000000 INFO @ Sat, 15 Jan 2022 18:52:35: 38000000 INFO @ Sat, 15 Jan 2022 18:52:35: 37000000 INFO @ Sat, 15 Jan 2022 18:52:38: 31000000 INFO @ Sat, 15 Jan 2022 18:52:40: 38000000 INFO @ Sat, 15 Jan 2022 18:52:40: 39000000 INFO @ Sat, 15 Jan 2022 18:52:42: 32000000 INFO @ Sat, 15 Jan 2022 18:52:44: 39000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:52:45: 40000000 INFO @ Sat, 15 Jan 2022 18:52:46: 33000000 INFO @ Sat, 15 Jan 2022 18:52:49: 40000000 INFO @ Sat, 15 Jan 2022 18:52:50: 41000000 INFO @ Sat, 15 Jan 2022 18:52:51: 34000000 INFO @ Sat, 15 Jan 2022 18:52:53: 41000000 INFO @ Sat, 15 Jan 2022 18:52:55: 42000000 INFO @ Sat, 15 Jan 2022 18:52:55: 35000000 INFO @ Sat, 15 Jan 2022 18:52:58: 42000000 INFO @ Sat, 15 Jan 2022 18:53:00: 36000000 INFO @ Sat, 15 Jan 2022 18:53:00: 43000000 INFO @ Sat, 15 Jan 2022 18:53:02: 43000000 INFO @ Sat, 15 Jan 2022 18:53:04: 37000000 INFO @ Sat, 15 Jan 2022 18:53:05: 44000000 INFO @ Sat, 15 Jan 2022 18:53:06: 44000000 INFO @ Sat, 15 Jan 2022 18:53:09: 38000000 INFO @ Sat, 15 Jan 2022 18:53:10: 45000000 INFO @ Sat, 15 Jan 2022 18:53:11: 45000000 INFO @ Sat, 15 Jan 2022 18:53:13: 39000000 INFO @ Sat, 15 Jan 2022 18:53:15: 46000000 INFO @ Sat, 15 Jan 2022 18:53:16: 46000000 INFO @ Sat, 15 Jan 2022 18:53:18: 40000000 INFO @ Sat, 15 Jan 2022 18:53:20: 47000000 INFO @ Sat, 15 Jan 2022 18:53:21: 47000000 INFO @ Sat, 15 Jan 2022 18:53:22: 41000000 INFO @ Sat, 15 Jan 2022 18:53:25: 48000000 INFO @ Sat, 15 Jan 2022 18:53:26: 48000000 INFO @ Sat, 15 Jan 2022 18:53:26: 42000000 INFO @ Sat, 15 Jan 2022 18:53:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:53:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:53:27: #1 total tags in treatment: 23374940 INFO @ Sat, 15 Jan 2022 18:53:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:53:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:53:27: #1 tags after filtering in treatment: 8025250 INFO @ Sat, 15 Jan 2022 18:53:27: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 18:53:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:53:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:53:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:53:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:53:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:53:28: #1 total tags in treatment: 23374940 INFO @ Sat, 15 Jan 2022 18:53:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:53:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:53:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:53:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:53:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:53:28: #1 tags after filtering in treatment: 8025250 INFO @ Sat, 15 Jan 2022 18:53:28: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 18:53:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:53:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:53:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:53:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:53:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:53:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:53:31: 43000000 INFO @ Sat, 15 Jan 2022 18:53:35: 44000000 INFO @ Sat, 15 Jan 2022 18:53:40: 45000000 INFO @ Sat, 15 Jan 2022 18:53:44: 46000000 INFO @ Sat, 15 Jan 2022 18:53:48: 47000000 INFO @ Sat, 15 Jan 2022 18:53:53: 48000000 INFO @ Sat, 15 Jan 2022 18:53:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:53:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:53:54: #1 total tags in treatment: 23374940 INFO @ Sat, 15 Jan 2022 18:53:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:53:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:53:55: #1 tags after filtering in treatment: 8025250 INFO @ Sat, 15 Jan 2022 18:53:55: #1 Redundant rate of treatment: 0.66 INFO @ Sat, 15 Jan 2022 18:53:55: #1 finished! INFO @ Sat, 15 Jan 2022 18:53:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:53:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:53:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:53:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:53:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829449/SRX8829449.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling