Job ID = 14519869 SRX = SRX8829418 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25659432 spots for SRR12329198/SRR12329198.sra Written 25659432 spots for SRR12329198/SRR12329198.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:52 25659432 reads; of these: 25659432 (100.00%) were paired; of these: 3660698 (14.27%) aligned concordantly 0 times 19655942 (76.60%) aligned concordantly exactly 1 time 2342792 (9.13%) aligned concordantly >1 times ---- 3660698 pairs aligned concordantly 0 times; of these: 544588 (14.88%) aligned discordantly 1 time ---- 3116110 pairs aligned 0 times concordantly or discordantly; of these: 6232220 mates make up the pairs; of these: 4106920 (65.90%) aligned 0 times 1774557 (28.47%) aligned exactly 1 time 350743 (5.63%) aligned >1 times 92.00% overall alignment rate Time searching: 00:22:52 Overall time: 00:22:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3404421 / 22454595 = 0.1516 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:22: 1000000 INFO @ Sat, 15 Jan 2022 18:35:27: 2000000 INFO @ Sat, 15 Jan 2022 18:35:33: 3000000 INFO @ Sat, 15 Jan 2022 18:35:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:45: 5000000 INFO @ Sat, 15 Jan 2022 18:35:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:51: 6000000 INFO @ Sat, 15 Jan 2022 18:35:52: 1000000 INFO @ Sat, 15 Jan 2022 18:35:57: 7000000 INFO @ Sat, 15 Jan 2022 18:35:58: 2000000 INFO @ Sat, 15 Jan 2022 18:36:03: 8000000 INFO @ Sat, 15 Jan 2022 18:36:04: 3000000 INFO @ Sat, 15 Jan 2022 18:36:10: 9000000 INFO @ Sat, 15 Jan 2022 18:36:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:36:16: 10000000 INFO @ Sat, 15 Jan 2022 18:36:16: 5000000 INFO @ Sat, 15 Jan 2022 18:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:36:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:36:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:36:21: 1000000 INFO @ Sat, 15 Jan 2022 18:36:22: 11000000 INFO @ Sat, 15 Jan 2022 18:36:22: 6000000 INFO @ Sat, 15 Jan 2022 18:36:27: 2000000 INFO @ Sat, 15 Jan 2022 18:36:28: 7000000 INFO @ Sat, 15 Jan 2022 18:36:28: 12000000 INFO @ Sat, 15 Jan 2022 18:36:32: 3000000 INFO @ Sat, 15 Jan 2022 18:36:34: 8000000 INFO @ Sat, 15 Jan 2022 18:36:35: 13000000 INFO @ Sat, 15 Jan 2022 18:36:37: 4000000 INFO @ Sat, 15 Jan 2022 18:36:40: 9000000 INFO @ Sat, 15 Jan 2022 18:36:41: 14000000 INFO @ Sat, 15 Jan 2022 18:36:43: 5000000 INFO @ Sat, 15 Jan 2022 18:36:46: 10000000 INFO @ Sat, 15 Jan 2022 18:36:48: 15000000 INFO @ Sat, 15 Jan 2022 18:36:48: 6000000 INFO @ Sat, 15 Jan 2022 18:36:52: 11000000 INFO @ Sat, 15 Jan 2022 18:36:53: 7000000 INFO @ Sat, 15 Jan 2022 18:36:54: 16000000 INFO @ Sat, 15 Jan 2022 18:36:59: 12000000 INFO @ Sat, 15 Jan 2022 18:36:59: 8000000 INFO @ Sat, 15 Jan 2022 18:37:01: 17000000 INFO @ Sat, 15 Jan 2022 18:37:04: 9000000 INFO @ Sat, 15 Jan 2022 18:37:05: 13000000 INFO @ Sat, 15 Jan 2022 18:37:07: 18000000 INFO @ Sat, 15 Jan 2022 18:37:09: 10000000 INFO @ Sat, 15 Jan 2022 18:37:11: 14000000 INFO @ Sat, 15 Jan 2022 18:37:14: 19000000 INFO @ Sat, 15 Jan 2022 18:37:15: 11000000 INFO @ Sat, 15 Jan 2022 18:37:18: 15000000 INFO @ Sat, 15 Jan 2022 18:37:20: 12000000 INFO @ Sat, 15 Jan 2022 18:37:20: 20000000 INFO @ Sat, 15 Jan 2022 18:37:24: 16000000 INFO @ Sat, 15 Jan 2022 18:37:25: 13000000 INFO @ Sat, 15 Jan 2022 18:37:27: 21000000 INFO @ Sat, 15 Jan 2022 18:37:30: 17000000 INFO @ Sat, 15 Jan 2022 18:37:31: 14000000 INFO @ Sat, 15 Jan 2022 18:37:33: 22000000 INFO @ Sat, 15 Jan 2022 18:37:36: 18000000 INFO @ Sat, 15 Jan 2022 18:37:37: 15000000 INFO @ Sat, 15 Jan 2022 18:37:39: 23000000 INFO @ Sat, 15 Jan 2022 18:37:43: 16000000 INFO @ Sat, 15 Jan 2022 18:37:43: 19000000 INFO @ Sat, 15 Jan 2022 18:37:46: 24000000 INFO @ Sat, 15 Jan 2022 18:37:49: 20000000 INFO @ Sat, 15 Jan 2022 18:37:49: 17000000 INFO @ Sat, 15 Jan 2022 18:37:52: 25000000 INFO @ Sat, 15 Jan 2022 18:37:55: 21000000 INFO @ Sat, 15 Jan 2022 18:37:56: 18000000 INFO @ Sat, 15 Jan 2022 18:37:59: 26000000 INFO @ Sat, 15 Jan 2022 18:38:02: 22000000 INFO @ Sat, 15 Jan 2022 18:38:02: 19000000 INFO @ Sat, 15 Jan 2022 18:38:05: 27000000 INFO @ Sat, 15 Jan 2022 18:38:08: 23000000 INFO @ Sat, 15 Jan 2022 18:38:09: 20000000 INFO @ Sat, 15 Jan 2022 18:38:12: 28000000 INFO @ Sat, 15 Jan 2022 18:38:15: 24000000 INFO @ Sat, 15 Jan 2022 18:38:15: 21000000 INFO @ Sat, 15 Jan 2022 18:38:19: 29000000 INFO @ Sat, 15 Jan 2022 18:38:21: 25000000 INFO @ Sat, 15 Jan 2022 18:38:22: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:38:26: 30000000 INFO @ Sat, 15 Jan 2022 18:38:28: 23000000 INFO @ Sat, 15 Jan 2022 18:38:28: 26000000 INFO @ Sat, 15 Jan 2022 18:38:33: 31000000 INFO @ Sat, 15 Jan 2022 18:38:35: 24000000 INFO @ Sat, 15 Jan 2022 18:38:35: 27000000 INFO @ Sat, 15 Jan 2022 18:38:39: 32000000 INFO @ Sat, 15 Jan 2022 18:38:41: 28000000 INFO @ Sat, 15 Jan 2022 18:38:42: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:38:46: 33000000 INFO @ Sat, 15 Jan 2022 18:38:48: 29000000 INFO @ Sat, 15 Jan 2022 18:38:49: 26000000 INFO @ Sat, 15 Jan 2022 18:38:53: 34000000 INFO @ Sat, 15 Jan 2022 18:38:54: 30000000 INFO @ Sat, 15 Jan 2022 18:38:56: 27000000 INFO @ Sat, 15 Jan 2022 18:39:00: 35000000 INFO @ Sat, 15 Jan 2022 18:39:01: 31000000 INFO @ Sat, 15 Jan 2022 18:39:04: 28000000 INFO @ Sat, 15 Jan 2022 18:39:07: 36000000 INFO @ Sat, 15 Jan 2022 18:39:08: 32000000 INFO @ Sat, 15 Jan 2022 18:39:11: 29000000 INFO @ Sat, 15 Jan 2022 18:39:14: 37000000 INFO @ Sat, 15 Jan 2022 18:39:15: 33000000 INFO @ Sat, 15 Jan 2022 18:39:18: 30000000 INFO @ Sat, 15 Jan 2022 18:39:21: 38000000 INFO @ Sat, 15 Jan 2022 18:39:22: 34000000 INFO @ Sat, 15 Jan 2022 18:39:25: 31000000 INFO @ Sat, 15 Jan 2022 18:39:28: 39000000 INFO @ Sat, 15 Jan 2022 18:39:29: 35000000 INFO @ Sat, 15 Jan 2022 18:39:33: 32000000 INFO @ Sat, 15 Jan 2022 18:39:34: 40000000 INFO @ Sat, 15 Jan 2022 18:39:35: 36000000 INFO @ Sat, 15 Jan 2022 18:39:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:39:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:39:37: #1 total tags in treatment: 18636157 INFO @ Sat, 15 Jan 2022 18:39:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:38: #1 tags after filtering in treatment: 9079203 INFO @ Sat, 15 Jan 2022 18:39:38: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 18:39:38: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:39:40: 33000000 INFO @ Sat, 15 Jan 2022 18:39:42: 37000000 INFO @ Sat, 15 Jan 2022 18:39:47: 34000000 INFO @ Sat, 15 Jan 2022 18:39:50: 38000000 INFO @ Sat, 15 Jan 2022 18:39:54: 35000000 INFO @ Sat, 15 Jan 2022 18:39:56: 39000000 INFO @ Sat, 15 Jan 2022 18:40:01: 36000000 INFO @ Sat, 15 Jan 2022 18:40:02: 40000000 INFO @ Sat, 15 Jan 2022 18:40:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:05: #1 total tags in treatment: 18636157 INFO @ Sat, 15 Jan 2022 18:40:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:06: #1 tags after filtering in treatment: 9079203 INFO @ Sat, 15 Jan 2022 18:40:06: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 18:40:06: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:40:08: 37000000 INFO @ Sat, 15 Jan 2022 18:40:16: 38000000 INFO @ Sat, 15 Jan 2022 18:40:23: 39000000 INFO @ Sat, 15 Jan 2022 18:40:30: 40000000 INFO @ Sat, 15 Jan 2022 18:40:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:33: #1 total tags in treatment: 18636157 INFO @ Sat, 15 Jan 2022 18:40:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:34: #1 tags after filtering in treatment: 9079203 INFO @ Sat, 15 Jan 2022 18:40:34: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 18:40:34: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829418/SRX8829418.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling