Job ID = 14519867 SRX = SRX8829416 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19521005 spots for SRR12329196/SRR12329196.sra Written 19521005 spots for SRR12329196/SRR12329196.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:16 19521005 reads; of these: 19521005 (100.00%) were paired; of these: 2381785 (12.20%) aligned concordantly 0 times 10296307 (52.74%) aligned concordantly exactly 1 time 6842913 (35.05%) aligned concordantly >1 times ---- 2381785 pairs aligned concordantly 0 times; of these: 130787 (5.49%) aligned discordantly 1 time ---- 2250998 pairs aligned 0 times concordantly or discordantly; of these: 4501996 mates make up the pairs; of these: 3230055 (71.75%) aligned 0 times 652277 (14.49%) aligned exactly 1 time 619664 (13.76%) aligned >1 times 91.73% overall alignment rate Time searching: 00:10:16 Overall time: 00:10:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 13074875 / 17236880 = 0.7585 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:03: 1000000 INFO @ Sat, 15 Jan 2022 18:12:07: 2000000 INFO @ Sat, 15 Jan 2022 18:12:12: 3000000 INFO @ Sat, 15 Jan 2022 18:12:16: 4000000 INFO @ Sat, 15 Jan 2022 18:12:20: 5000000 INFO @ Sat, 15 Jan 2022 18:12:24: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:28: 7000000 INFO @ Sat, 15 Jan 2022 18:12:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:33: 8000000 INFO @ Sat, 15 Jan 2022 18:12:33: 1000000 INFO @ Sat, 15 Jan 2022 18:12:37: 9000000 INFO @ Sat, 15 Jan 2022 18:12:38: 2000000 INFO @ Sat, 15 Jan 2022 18:12:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:40: #1 total tags in treatment: 4116030 INFO @ Sat, 15 Jan 2022 18:12:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:40: #1 tags after filtering in treatment: 2474596 INFO @ Sat, 15 Jan 2022 18:12:40: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 18:12:40: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:40: #2 number of paired peaks: 37 WARNING @ Sat, 15 Jan 2022 18:12:40: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:12:42: 3000000 INFO @ Sat, 15 Jan 2022 18:12:46: 4000000 INFO @ Sat, 15 Jan 2022 18:12:51: 5000000 INFO @ Sat, 15 Jan 2022 18:12:55: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:59: 7000000 INFO @ Sat, 15 Jan 2022 18:13:03: 1000000 INFO @ Sat, 15 Jan 2022 18:13:04: 8000000 INFO @ Sat, 15 Jan 2022 18:13:08: 2000000 INFO @ Sat, 15 Jan 2022 18:13:08: 9000000 INFO @ Sat, 15 Jan 2022 18:13:11: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:11: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:11: #1 total tags in treatment: 4116030 INFO @ Sat, 15 Jan 2022 18:13:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:11: #1 tags after filtering in treatment: 2474596 INFO @ Sat, 15 Jan 2022 18:13:11: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 18:13:11: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:11: #2 number of paired peaks: 37 WARNING @ Sat, 15 Jan 2022 18:13:11: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:13:12: 3000000 INFO @ Sat, 15 Jan 2022 18:13:16: 4000000 INFO @ Sat, 15 Jan 2022 18:13:21: 5000000 INFO @ Sat, 15 Jan 2022 18:13:25: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:13:29: 7000000 INFO @ Sat, 15 Jan 2022 18:13:34: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:13:38: 9000000 INFO @ Sat, 15 Jan 2022 18:13:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:41: #1 total tags in treatment: 4116030 INFO @ Sat, 15 Jan 2022 18:13:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:41: #1 tags after filtering in treatment: 2474596 INFO @ Sat, 15 Jan 2022 18:13:41: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 18:13:41: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:41: #2 number of paired peaks: 37 WARNING @ Sat, 15 Jan 2022 18:13:41: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829416/SRX8829416.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling