Job ID = 14519861 SRX = SRX8829412 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26355336 spots for SRR12329192/SRR12329192.sra Written 26355336 spots for SRR12329192/SRR12329192.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:53 26355336 reads; of these: 26355336 (100.00%) were paired; of these: 3729205 (14.15%) aligned concordantly 0 times 19926463 (75.61%) aligned concordantly exactly 1 time 2699668 (10.24%) aligned concordantly >1 times ---- 3729205 pairs aligned concordantly 0 times; of these: 458963 (12.31%) aligned discordantly 1 time ---- 3270242 pairs aligned 0 times concordantly or discordantly; of these: 6540484 mates make up the pairs; of these: 4240260 (64.83%) aligned 0 times 1898759 (29.03%) aligned exactly 1 time 401465 (6.14%) aligned >1 times 91.96% overall alignment rate Time searching: 00:15:53 Overall time: 00:15:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3648707 / 22998203 = 0.1587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:24:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:24:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:24:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:24:53: 1000000 INFO @ Sat, 15 Jan 2022 18:25:00: 2000000 INFO @ Sat, 15 Jan 2022 18:25:06: 3000000 INFO @ Sat, 15 Jan 2022 18:25:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:20: 5000000 INFO @ Sat, 15 Jan 2022 18:25:23: 1000000 INFO @ Sat, 15 Jan 2022 18:25:28: 6000000 INFO @ Sat, 15 Jan 2022 18:25:29: 2000000 INFO @ Sat, 15 Jan 2022 18:25:35: 7000000 INFO @ Sat, 15 Jan 2022 18:25:36: 3000000 INFO @ Sat, 15 Jan 2022 18:25:42: 4000000 INFO @ Sat, 15 Jan 2022 18:25:43: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:25:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:25:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:25:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:25:49: 5000000 INFO @ Sat, 15 Jan 2022 18:25:50: 9000000 INFO @ Sat, 15 Jan 2022 18:25:53: 1000000 INFO @ Sat, 15 Jan 2022 18:25:55: 6000000 INFO @ Sat, 15 Jan 2022 18:25:57: 10000000 INFO @ Sat, 15 Jan 2022 18:25:59: 2000000 INFO @ Sat, 15 Jan 2022 18:26:02: 7000000 INFO @ Sat, 15 Jan 2022 18:26:05: 11000000 INFO @ Sat, 15 Jan 2022 18:26:06: 3000000 INFO @ Sat, 15 Jan 2022 18:26:08: 8000000 INFO @ Sat, 15 Jan 2022 18:26:12: 4000000 INFO @ Sat, 15 Jan 2022 18:26:13: 12000000 INFO @ Sat, 15 Jan 2022 18:26:15: 9000000 INFO @ Sat, 15 Jan 2022 18:26:19: 5000000 INFO @ Sat, 15 Jan 2022 18:26:20: 13000000 INFO @ Sat, 15 Jan 2022 18:26:22: 10000000 INFO @ Sat, 15 Jan 2022 18:26:25: 6000000 INFO @ Sat, 15 Jan 2022 18:26:27: 14000000 INFO @ Sat, 15 Jan 2022 18:26:28: 11000000 INFO @ Sat, 15 Jan 2022 18:26:31: 7000000 INFO @ Sat, 15 Jan 2022 18:26:35: 15000000 INFO @ Sat, 15 Jan 2022 18:26:36: 12000000 INFO @ Sat, 15 Jan 2022 18:26:38: 8000000 INFO @ Sat, 15 Jan 2022 18:26:42: 16000000 INFO @ Sat, 15 Jan 2022 18:26:43: 13000000 INFO @ Sat, 15 Jan 2022 18:26:44: 9000000 INFO @ Sat, 15 Jan 2022 18:26:49: 14000000 INFO @ Sat, 15 Jan 2022 18:26:50: 17000000 INFO @ Sat, 15 Jan 2022 18:26:51: 10000000 INFO @ Sat, 15 Jan 2022 18:26:56: 15000000 INFO @ Sat, 15 Jan 2022 18:26:57: 11000000 INFO @ Sat, 15 Jan 2022 18:26:57: 18000000 INFO @ Sat, 15 Jan 2022 18:27:02: 16000000 INFO @ Sat, 15 Jan 2022 18:27:03: 12000000 INFO @ Sat, 15 Jan 2022 18:27:05: 19000000 INFO @ Sat, 15 Jan 2022 18:27:09: 17000000 INFO @ Sat, 15 Jan 2022 18:27:10: 13000000 INFO @ Sat, 15 Jan 2022 18:27:12: 20000000 INFO @ Sat, 15 Jan 2022 18:27:15: 18000000 INFO @ Sat, 15 Jan 2022 18:27:16: 14000000 INFO @ Sat, 15 Jan 2022 18:27:20: 21000000 INFO @ Sat, 15 Jan 2022 18:27:21: 19000000 INFO @ Sat, 15 Jan 2022 18:27:23: 15000000 INFO @ Sat, 15 Jan 2022 18:27:27: 22000000 INFO @ Sat, 15 Jan 2022 18:27:28: 20000000 INFO @ Sat, 15 Jan 2022 18:27:29: 16000000 INFO @ Sat, 15 Jan 2022 18:27:34: 23000000 INFO @ Sat, 15 Jan 2022 18:27:34: 21000000 INFO @ Sat, 15 Jan 2022 18:27:36: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:27:41: 22000000 INFO @ Sat, 15 Jan 2022 18:27:42: 24000000 INFO @ Sat, 15 Jan 2022 18:27:42: 18000000 INFO @ Sat, 15 Jan 2022 18:27:47: 23000000 INFO @ Sat, 15 Jan 2022 18:27:48: 19000000 INFO @ Sat, 15 Jan 2022 18:27:49: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:27:54: 24000000 INFO @ Sat, 15 Jan 2022 18:27:55: 20000000 INFO @ Sat, 15 Jan 2022 18:27:57: 26000000 INFO @ Sat, 15 Jan 2022 18:28:00: 25000000 INFO @ Sat, 15 Jan 2022 18:28:01: 21000000 INFO @ Sat, 15 Jan 2022 18:28:04: 27000000 INFO @ Sat, 15 Jan 2022 18:28:06: 26000000 INFO @ Sat, 15 Jan 2022 18:28:08: 22000000 INFO @ Sat, 15 Jan 2022 18:28:11: 28000000 INFO @ Sat, 15 Jan 2022 18:28:13: 27000000 INFO @ Sat, 15 Jan 2022 18:28:14: 23000000 INFO @ Sat, 15 Jan 2022 18:28:19: 29000000 INFO @ Sat, 15 Jan 2022 18:28:19: 28000000 INFO @ Sat, 15 Jan 2022 18:28:20: 24000000 INFO @ Sat, 15 Jan 2022 18:28:26: 29000000 INFO @ Sat, 15 Jan 2022 18:28:26: 30000000 INFO @ Sat, 15 Jan 2022 18:28:27: 25000000 INFO @ Sat, 15 Jan 2022 18:28:32: 30000000 INFO @ Sat, 15 Jan 2022 18:28:33: 26000000 INFO @ Sat, 15 Jan 2022 18:28:33: 31000000 INFO @ Sat, 15 Jan 2022 18:28:38: 31000000 INFO @ Sat, 15 Jan 2022 18:28:39: 27000000 INFO @ Sat, 15 Jan 2022 18:28:41: 32000000 INFO @ Sat, 15 Jan 2022 18:28:45: 32000000 INFO @ Sat, 15 Jan 2022 18:28:46: 28000000 INFO @ Sat, 15 Jan 2022 18:28:48: 33000000 INFO @ Sat, 15 Jan 2022 18:28:51: 33000000 INFO @ Sat, 15 Jan 2022 18:28:52: 29000000 INFO @ Sat, 15 Jan 2022 18:28:55: 34000000 INFO @ Sat, 15 Jan 2022 18:28:58: 34000000 INFO @ Sat, 15 Jan 2022 18:28:58: 30000000 INFO @ Sat, 15 Jan 2022 18:29:03: 35000000 INFO @ Sat, 15 Jan 2022 18:29:04: 35000000 INFO @ Sat, 15 Jan 2022 18:29:05: 31000000 INFO @ Sat, 15 Jan 2022 18:29:10: 36000000 INFO @ Sat, 15 Jan 2022 18:29:11: 36000000 INFO @ Sat, 15 Jan 2022 18:29:11: 32000000 INFO @ Sat, 15 Jan 2022 18:29:17: 33000000 INFO @ Sat, 15 Jan 2022 18:29:18: 37000000 INFO @ Sat, 15 Jan 2022 18:29:18: 37000000 INFO @ Sat, 15 Jan 2022 18:29:23: 34000000 INFO @ Sat, 15 Jan 2022 18:29:25: 38000000 INFO @ Sat, 15 Jan 2022 18:29:26: 38000000 INFO @ Sat, 15 Jan 2022 18:29:30: 35000000 INFO @ Sat, 15 Jan 2022 18:29:33: 39000000 INFO @ Sat, 15 Jan 2022 18:29:33: 39000000 INFO @ Sat, 15 Jan 2022 18:29:36: 36000000 INFO @ Sat, 15 Jan 2022 18:29:41: 40000000 INFO @ Sat, 15 Jan 2022 18:29:41: 40000000 INFO @ Sat, 15 Jan 2022 18:29:42: 37000000 INFO @ Sat, 15 Jan 2022 18:29:48: 41000000 INFO @ Sat, 15 Jan 2022 18:29:48: 38000000 INFO @ Sat, 15 Jan 2022 18:29:49: 41000000 INFO @ Sat, 15 Jan 2022 18:29:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:29:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:29:49: #1 total tags in treatment: 19011639 INFO @ Sat, 15 Jan 2022 18:29:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:50: #1 tags after filtering in treatment: 9014646 INFO @ Sat, 15 Jan 2022 18:29:50: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:29:50: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:29:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:29:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:29:51: #1 total tags in treatment: 19011639 INFO @ Sat, 15 Jan 2022 18:29:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:51: #1 tags after filtering in treatment: 9014646 INFO @ Sat, 15 Jan 2022 18:29:51: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:29:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:29:54: 39000000 INFO @ Sat, 15 Jan 2022 18:29:59: 40000000 INFO @ Sat, 15 Jan 2022 18:30:04: 41000000 INFO @ Sat, 15 Jan 2022 18:30:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:30:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:30:05: #1 total tags in treatment: 19011639 INFO @ Sat, 15 Jan 2022 18:30:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:05: #1 tags after filtering in treatment: 9014646 INFO @ Sat, 15 Jan 2022 18:30:05: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:30:05: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829412/SRX8829412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling