Job ID = 11193205 sra ファイルのダウンロード中... Completed: 283351K bytes transferred in 5 seconds (394154K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 6491112 spots for /home/okishinya/chipatlas/results/sacCer3/SRX876293/SRR1802396.sra Written 6491112 spots for /home/okishinya/chipatlas/results/sacCer3/SRX876293/SRR1802396.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 6491112 reads; of these: 6491112 (100.00%) were paired; of these: 3950817 (60.87%) aligned concordantly 0 times 2110609 (32.52%) aligned concordantly exactly 1 time 429686 (6.62%) aligned concordantly >1 times ---- 3950817 pairs aligned concordantly 0 times; of these: 62404 (1.58%) aligned discordantly 1 time ---- 3888413 pairs aligned 0 times concordantly or discordantly; of these: 7776826 mates make up the pairs; of these: 7633097 (98.15%) aligned 0 times 93047 (1.20%) aligned exactly 1 time 50682 (0.65%) aligned >1 times 41.20% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1821401 / 2583968 = 0.7049 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:12:05: # Command line: callpeak -t SRX876293.bam -f BAM -g 12100000 -n SRX876293.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX876293.05 # format = BAM # ChIP-seq file = ['SRX876293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:12:05: # Command line: callpeak -t SRX876293.bam -f BAM -g 12100000 -n SRX876293.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX876293.20 # format = BAM # ChIP-seq file = ['SRX876293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:12:05: # Command line: callpeak -t SRX876293.bam -f BAM -g 12100000 -n SRX876293.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX876293.10 # format = BAM # ChIP-seq file = ['SRX876293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:12:05: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:12:05: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:12:05: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:12:05: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:12:05: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:12:05: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:12:11: 1000000 INFO @ Sat, 15 Sep 2018 11:12:11: 1000000 INFO @ Sat, 15 Sep 2018 11:12:12: 1000000 INFO @ Sat, 15 Sep 2018 11:12:16: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:12:16: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:12:16: #1 total tags in treatment: 758124 INFO @ Sat, 15 Sep 2018 11:12:16: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:12:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:12:16: #1 tags after filtering in treatment: 645157 INFO @ Sat, 15 Sep 2018 11:12:16: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Sep 2018 11:12:16: #1 finished! INFO @ Sat, 15 Sep 2018 11:12:16: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:12:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:12:16: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:12:16: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:12:16: #1 total tags in treatment: 758124 INFO @ Sat, 15 Sep 2018 11:12:16: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:12:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:12:16: #2 number of paired peaks: 60 WARNING @ Sat, 15 Sep 2018 11:12:16: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:12:16: Process for pairing-model is terminated! cat: SRX876293.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Sat, 15 Sep 2018 11:12:16: #1 tags after filtering in treatment: 645157 INFO @ Sat, 15 Sep 2018 11:12:16: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Sep 2018 11:12:16: #1 finished! INFO @ Sat, 15 Sep 2018 11:12:16: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:12:16: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX876293.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX876293.20_*.xls': そのようなファイルやディレクトリはありません INFO @ Sat, 15 Sep 2018 11:12:16: #2 number of paired peaks: 60 WARNING @ Sat, 15 Sep 2018 11:12:16: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:12:16: Process for pairing-model is terminated! rm: cannot remove `SRX876293.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX876293.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX876293.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX876293.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX876293.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:12:17: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:12:17: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:12:17: #1 total tags in treatment: 758124 INFO @ Sat, 15 Sep 2018 11:12:17: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:12:17: #1 tags after filtering in treatment: 645157 INFO @ Sat, 15 Sep 2018 11:12:17: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 15 Sep 2018 11:12:17: #1 finished! INFO @ Sat, 15 Sep 2018 11:12:17: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:12:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:12:17: #2 number of paired peaks: 60 WARNING @ Sat, 15 Sep 2018 11:12:17: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:12:17: Process for pairing-model is terminated! cat: SRX876293.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX876293.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX876293.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX876293.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。