Job ID = 10224030 SRX = SRX8754219 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8025725 spots for SRR12246290/SRR12246290.sra Written 8025725 spots for SRR12246290/SRR12246290.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:07 8025725 reads; of these: 8025725 (100.00%) were paired; of these: 468475 (5.84%) aligned concordantly 0 times 7021449 (87.49%) aligned concordantly exactly 1 time 535801 (6.68%) aligned concordantly >1 times ---- 468475 pairs aligned concordantly 0 times; of these: 151362 (32.31%) aligned discordantly 1 time ---- 317113 pairs aligned 0 times concordantly or discordantly; of these: 634226 mates make up the pairs; of these: 466190 (73.51%) aligned 0 times 134551 (21.21%) aligned exactly 1 time 33485 (5.28%) aligned >1 times 97.10% overall alignment rate Time searching: 00:04:07 Overall time: 00:04:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 305424 / 7699997 = 0.0397 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:18:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:18:52: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:18:52: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:18:58: 1000000 INFO @ Fri, 16 Oct 2020 09:19:04: 2000000 INFO @ Fri, 16 Oct 2020 09:19:09: 3000000 INFO @ Fri, 16 Oct 2020 09:19:15: 4000000 INFO @ Fri, 16 Oct 2020 09:19:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:19:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:19:22: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:19:22: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:19:26: 6000000 INFO @ Fri, 16 Oct 2020 09:19:29: 1000000 INFO @ Fri, 16 Oct 2020 09:19:32: 7000000 INFO @ Fri, 16 Oct 2020 09:19:36: 2000000 INFO @ Fri, 16 Oct 2020 09:19:39: 8000000 INFO @ Fri, 16 Oct 2020 09:19:42: 3000000 INFO @ Fri, 16 Oct 2020 09:19:46: 9000000 INFO @ Fri, 16 Oct 2020 09:19:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:19:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:19:52: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:19:52: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:19:53: 10000000 INFO @ Fri, 16 Oct 2020 09:19:55: 5000000 INFO @ Fri, 16 Oct 2020 09:19:59: 1000000 INFO @ Fri, 16 Oct 2020 09:20:00: 11000000 INFO @ Fri, 16 Oct 2020 09:20:01: 6000000 INFO @ Fri, 16 Oct 2020 09:20:06: 2000000 INFO @ Fri, 16 Oct 2020 09:20:06: 12000000 INFO @ Fri, 16 Oct 2020 09:20:07: 7000000 INFO @ Fri, 16 Oct 2020 09:20:13: 3000000 INFO @ Fri, 16 Oct 2020 09:20:13: 13000000 INFO @ Fri, 16 Oct 2020 09:20:14: 8000000 INFO @ Fri, 16 Oct 2020 09:20:19: 4000000 INFO @ Fri, 16 Oct 2020 09:20:20: 14000000 INFO @ Fri, 16 Oct 2020 09:20:21: 9000000 INFO @ Fri, 16 Oct 2020 09:20:26: 5000000 INFO @ Fri, 16 Oct 2020 09:20:27: #1 tag size is determined as 51 bps INFO @ Fri, 16 Oct 2020 09:20:27: #1 tag size = 51 INFO @ Fri, 16 Oct 2020 09:20:27: #1 total tags in treatment: 7253192 INFO @ Fri, 16 Oct 2020 09:20:27: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:20:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:20:27: #1 tags after filtering in treatment: 5822414 INFO @ Fri, 16 Oct 2020 09:20:27: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 16 Oct 2020 09:20:27: #1 finished! INFO @ Fri, 16 Oct 2020 09:20:27: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:20:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:20:27: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:20:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:20:27: Process for pairing-model is terminated! INFO @ Fri, 16 Oct 2020 09:20:28: 10000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:20:32: 6000000 INFO @ Fri, 16 Oct 2020 09:20:36: 11000000 INFO @ Fri, 16 Oct 2020 09:20:39: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:20:43: 12000000 INFO @ Fri, 16 Oct 2020 09:20:46: 8000000 INFO @ Fri, 16 Oct 2020 09:20:50: 13000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:20:54: 9000000 INFO @ Fri, 16 Oct 2020 09:20:57: 14000000 INFO @ Fri, 16 Oct 2020 09:21:01: 10000000 INFO @ Fri, 16 Oct 2020 09:21:03: #1 tag size is determined as 51 bps INFO @ Fri, 16 Oct 2020 09:21:03: #1 tag size = 51 INFO @ Fri, 16 Oct 2020 09:21:03: #1 total tags in treatment: 7253192 INFO @ Fri, 16 Oct 2020 09:21:03: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:21:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:21:03: #1 tags after filtering in treatment: 5822414 INFO @ Fri, 16 Oct 2020 09:21:03: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 16 Oct 2020 09:21:03: #1 finished! INFO @ Fri, 16 Oct 2020 09:21:03: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:21:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:21:04: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:21:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:21:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:21:08: 11000000 INFO @ Fri, 16 Oct 2020 09:21:15: 12000000 INFO @ Fri, 16 Oct 2020 09:21:22: 13000000 INFO @ Fri, 16 Oct 2020 09:21:28: 14000000 INFO @ Fri, 16 Oct 2020 09:21:34: #1 tag size is determined as 51 bps INFO @ Fri, 16 Oct 2020 09:21:34: #1 tag size = 51 INFO @ Fri, 16 Oct 2020 09:21:34: #1 total tags in treatment: 7253192 INFO @ Fri, 16 Oct 2020 09:21:34: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:21:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:21:34: #1 tags after filtering in treatment: 5822414 INFO @ Fri, 16 Oct 2020 09:21:34: #1 Redundant rate of treatment: 0.20 INFO @ Fri, 16 Oct 2020 09:21:34: #1 finished! INFO @ Fri, 16 Oct 2020 09:21:34: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:21:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:21:35: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:21:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:21:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling