Job ID = 14519767 SRX = SRX8754219 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8025725 spots for SRR12246290/SRR12246290.sra Written 8025725 spots for SRR12246290/SRR12246290.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:11 8025725 reads; of these: 8025725 (100.00%) were paired; of these: 468475 (5.84%) aligned concordantly 0 times 7021449 (87.49%) aligned concordantly exactly 1 time 535801 (6.68%) aligned concordantly >1 times ---- 468475 pairs aligned concordantly 0 times; of these: 151362 (32.31%) aligned discordantly 1 time ---- 317113 pairs aligned 0 times concordantly or discordantly; of these: 634226 mates make up the pairs; of these: 466190 (73.51%) aligned 0 times 134551 (21.21%) aligned exactly 1 time 33485 (5.28%) aligned >1 times 97.10% overall alignment rate Time searching: 00:06:11 Overall time: 00:06:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 305424 / 7699997 = 0.0397 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:53:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:53:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:53:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:53:34: 1000000 INFO @ Sat, 15 Jan 2022 17:53:41: 2000000 INFO @ Sat, 15 Jan 2022 17:53:49: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:53:56: 4000000 INFO @ Sat, 15 Jan 2022 17:53:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:53:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:53:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:54:05: 5000000 INFO @ Sat, 15 Jan 2022 17:54:06: 1000000 INFO @ Sat, 15 Jan 2022 17:54:14: 2000000 INFO @ Sat, 15 Jan 2022 17:54:15: 6000000 INFO @ Sat, 15 Jan 2022 17:54:22: 3000000 INFO @ Sat, 15 Jan 2022 17:54:24: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:54:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:54:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:54:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:54:31: 4000000 INFO @ Sat, 15 Jan 2022 17:54:33: 8000000 INFO @ Sat, 15 Jan 2022 17:54:35: 1000000 INFO @ Sat, 15 Jan 2022 17:54:39: 5000000 INFO @ Sat, 15 Jan 2022 17:54:42: 2000000 INFO @ Sat, 15 Jan 2022 17:54:43: 9000000 INFO @ Sat, 15 Jan 2022 17:54:48: 6000000 INFO @ Sat, 15 Jan 2022 17:54:50: 3000000 INFO @ Sat, 15 Jan 2022 17:54:52: 10000000 INFO @ Sat, 15 Jan 2022 17:54:57: 7000000 INFO @ Sat, 15 Jan 2022 17:54:58: 4000000 INFO @ Sat, 15 Jan 2022 17:55:01: 11000000 INFO @ Sat, 15 Jan 2022 17:55:06: 5000000 INFO @ Sat, 15 Jan 2022 17:55:06: 8000000 INFO @ Sat, 15 Jan 2022 17:55:11: 12000000 INFO @ Sat, 15 Jan 2022 17:55:13: 6000000 INFO @ Sat, 15 Jan 2022 17:55:15: 9000000 INFO @ Sat, 15 Jan 2022 17:55:20: 13000000 INFO @ Sat, 15 Jan 2022 17:55:21: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:55:23: 10000000 INFO @ Sat, 15 Jan 2022 17:55:28: 8000000 INFO @ Sat, 15 Jan 2022 17:55:29: 14000000 INFO @ Sat, 15 Jan 2022 17:55:32: 11000000 INFO @ Sat, 15 Jan 2022 17:55:35: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:55:37: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:55:37: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:55:37: #1 total tags in treatment: 7253192 INFO @ Sat, 15 Jan 2022 17:55:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:55:38: #1 tags after filtering in treatment: 5822414 INFO @ Sat, 15 Jan 2022 17:55:38: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:55:38: #1 finished! INFO @ Sat, 15 Jan 2022 17:55:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:55:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:55:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:55:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:55:40: 12000000 INFO @ Sat, 15 Jan 2022 17:55:43: 10000000 INFO @ Sat, 15 Jan 2022 17:55:49: 13000000 INFO @ Sat, 15 Jan 2022 17:55:50: 11000000 INFO @ Sat, 15 Jan 2022 17:55:57: 14000000 INFO @ Sat, 15 Jan 2022 17:55:57: 12000000 INFO @ Sat, 15 Jan 2022 17:56:05: 13000000 INFO @ Sat, 15 Jan 2022 17:56:05: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:56:05: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:56:05: #1 total tags in treatment: 7253192 INFO @ Sat, 15 Jan 2022 17:56:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:56:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:56:05: #1 tags after filtering in treatment: 5822414 INFO @ Sat, 15 Jan 2022 17:56:05: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:56:05: #1 finished! INFO @ Sat, 15 Jan 2022 17:56:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:56:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:56:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:56:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:56:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:56:12: 14000000 INFO @ Sat, 15 Jan 2022 17:56:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:56:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:56:19: #1 total tags in treatment: 7253192 INFO @ Sat, 15 Jan 2022 17:56:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:56:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:56:19: #1 tags after filtering in treatment: 5822414 INFO @ Sat, 15 Jan 2022 17:56:19: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:56:19: #1 finished! INFO @ Sat, 15 Jan 2022 17:56:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:56:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:56:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:56:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:56:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754219/SRX8754219.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling