Job ID = 14519733 SRX = SRX8754205 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14937357 spots for SRR12246276/SRR12246276.sra Written 14937357 spots for SRR12246276/SRR12246276.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:12:55 14937357 reads; of these: 14937357 (100.00%) were paired; of these: 814863 (5.46%) aligned concordantly 0 times 11930612 (79.87%) aligned concordantly exactly 1 time 2191882 (14.67%) aligned concordantly >1 times ---- 814863 pairs aligned concordantly 0 times; of these: 140698 (17.27%) aligned discordantly 1 time ---- 674165 pairs aligned 0 times concordantly or discordantly; of these: 1348330 mates make up the pairs; of these: 1086764 (80.60%) aligned 0 times 163106 (12.10%) aligned exactly 1 time 98460 (7.30%) aligned >1 times 96.36% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2914658 / 14210809 = 0.2051 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:55:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:55:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:55:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:56:06: 1000000 INFO @ Sat, 15 Jan 2022 17:56:13: 2000000 INFO @ Sat, 15 Jan 2022 17:56:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:56:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:56:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:56:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:56:28: 4000000 INFO @ Sat, 15 Jan 2022 17:56:35: 1000000 INFO @ Sat, 15 Jan 2022 17:56:35: 5000000 INFO @ Sat, 15 Jan 2022 17:56:43: 6000000 INFO @ Sat, 15 Jan 2022 17:56:43: 2000000 INFO @ Sat, 15 Jan 2022 17:56:50: 7000000 INFO @ Sat, 15 Jan 2022 17:56:50: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:56:57: 8000000 INFO @ Sat, 15 Jan 2022 17:56:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:56:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:56:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:56:58: 4000000 INFO @ Sat, 15 Jan 2022 17:57:05: 1000000 INFO @ Sat, 15 Jan 2022 17:57:05: 5000000 INFO @ Sat, 15 Jan 2022 17:57:06: 9000000 INFO @ Sat, 15 Jan 2022 17:57:12: 2000000 INFO @ Sat, 15 Jan 2022 17:57:13: 6000000 INFO @ Sat, 15 Jan 2022 17:57:15: 10000000 INFO @ Sat, 15 Jan 2022 17:57:20: 3000000 INFO @ Sat, 15 Jan 2022 17:57:20: 7000000 INFO @ Sat, 15 Jan 2022 17:57:22: 11000000 INFO @ Sat, 15 Jan 2022 17:57:28: 4000000 INFO @ Sat, 15 Jan 2022 17:57:28: 8000000 INFO @ Sat, 15 Jan 2022 17:57:30: 12000000 INFO @ Sat, 15 Jan 2022 17:57:35: 5000000 INFO @ Sat, 15 Jan 2022 17:57:35: 9000000 INFO @ Sat, 15 Jan 2022 17:57:37: 13000000 INFO @ Sat, 15 Jan 2022 17:57:43: 6000000 INFO @ Sat, 15 Jan 2022 17:57:43: 10000000 INFO @ Sat, 15 Jan 2022 17:57:44: 14000000 INFO @ Sat, 15 Jan 2022 17:57:50: 7000000 INFO @ Sat, 15 Jan 2022 17:57:50: 11000000 INFO @ Sat, 15 Jan 2022 17:57:51: 15000000 INFO @ Sat, 15 Jan 2022 17:57:57: 8000000 INFO @ Sat, 15 Jan 2022 17:57:58: 12000000 INFO @ Sat, 15 Jan 2022 17:57:59: 16000000 INFO @ Sat, 15 Jan 2022 17:58:05: 9000000 INFO @ Sat, 15 Jan 2022 17:58:05: 13000000 INFO @ Sat, 15 Jan 2022 17:58:06: 17000000 INFO @ Sat, 15 Jan 2022 17:58:12: 10000000 INFO @ Sat, 15 Jan 2022 17:58:13: 18000000 INFO @ Sat, 15 Jan 2022 17:58:13: 14000000 INFO @ Sat, 15 Jan 2022 17:58:20: 11000000 INFO @ Sat, 15 Jan 2022 17:58:20: 19000000 INFO @ Sat, 15 Jan 2022 17:58:20: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:58:27: 20000000 INFO @ Sat, 15 Jan 2022 17:58:27: 12000000 INFO @ Sat, 15 Jan 2022 17:58:28: 16000000 INFO @ Sat, 15 Jan 2022 17:58:34: 21000000 INFO @ Sat, 15 Jan 2022 17:58:34: 13000000 INFO @ Sat, 15 Jan 2022 17:58:35: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:58:41: 22000000 INFO @ Sat, 15 Jan 2022 17:58:42: 14000000 INFO @ Sat, 15 Jan 2022 17:58:42: 18000000 INFO @ Sat, 15 Jan 2022 17:58:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:58:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:58:48: #1 total tags in treatment: 11219447 INFO @ Sat, 15 Jan 2022 17:58:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:58:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:58:48: #1 tags after filtering in treatment: 5538593 INFO @ Sat, 15 Jan 2022 17:58:48: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 17:58:48: #1 finished! INFO @ Sat, 15 Jan 2022 17:58:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:58:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:58:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:58:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:58:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:58:49: 15000000 INFO @ Sat, 15 Jan 2022 17:58:50: 19000000 INFO @ Sat, 15 Jan 2022 17:58:57: 16000000 INFO @ Sat, 15 Jan 2022 17:58:57: 20000000 INFO @ Sat, 15 Jan 2022 17:59:04: 17000000 INFO @ Sat, 15 Jan 2022 17:59:04: 21000000 INFO @ Sat, 15 Jan 2022 17:59:11: 18000000 INFO @ Sat, 15 Jan 2022 17:59:12: 22000000 INFO @ Sat, 15 Jan 2022 17:59:18: 19000000 INFO @ Sat, 15 Jan 2022 17:59:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:59:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:59:19: #1 total tags in treatment: 11219447 INFO @ Sat, 15 Jan 2022 17:59:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:59:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:59:19: #1 tags after filtering in treatment: 5538593 INFO @ Sat, 15 Jan 2022 17:59:19: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 17:59:19: #1 finished! INFO @ Sat, 15 Jan 2022 17:59:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:59:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:59:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:59:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:59:25: 20000000 INFO @ Sat, 15 Jan 2022 17:59:32: 21000000 INFO @ Sat, 15 Jan 2022 17:59:39: 22000000 INFO @ Sat, 15 Jan 2022 17:59:46: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 17:59:46: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 17:59:46: #1 total tags in treatment: 11219447 INFO @ Sat, 15 Jan 2022 17:59:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:59:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:59:47: #1 tags after filtering in treatment: 5538593 INFO @ Sat, 15 Jan 2022 17:59:47: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 17:59:47: #1 finished! INFO @ Sat, 15 Jan 2022 17:59:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:59:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:59:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:59:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:59:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754205/SRX8754205.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling