Job ID = 10224010 SRX = SRX8754199 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3149037 spots for SRR12246270/SRR12246270.sra Written 3149037 spots for SRR12246270/SRR12246270.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:25 3149037 reads; of these: 3149037 (100.00%) were paired; of these: 265045 (8.42%) aligned concordantly 0 times 2422654 (76.93%) aligned concordantly exactly 1 time 461338 (14.65%) aligned concordantly >1 times ---- 265045 pairs aligned concordantly 0 times; of these: 135986 (51.31%) aligned discordantly 1 time ---- 129059 pairs aligned 0 times concordantly or discordantly; of these: 258118 mates make up the pairs; of these: 160716 (62.26%) aligned 0 times 31601 (12.24%) aligned exactly 1 time 65801 (25.49%) aligned >1 times 97.45% overall alignment rate Time searching: 00:01:25 Overall time: 00:01:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 37495 / 3017172 = 0.0124 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:08:40: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:08:40: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:08:44: 1000000 INFO @ Fri, 16 Oct 2020 09:08:48: 2000000 INFO @ Fri, 16 Oct 2020 09:08:53: 3000000 INFO @ Fri, 16 Oct 2020 09:08:57: 4000000 INFO @ Fri, 16 Oct 2020 09:09:02: 5000000 INFO @ Fri, 16 Oct 2020 09:09:06: 6000000 INFO @ Fri, 16 Oct 2020 09:09:06: #1 tag size is determined as 38 bps INFO @ Fri, 16 Oct 2020 09:09:06: #1 tag size = 38 INFO @ Fri, 16 Oct 2020 09:09:06: #1 total tags in treatment: 2847267 INFO @ Fri, 16 Oct 2020 09:09:06: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:09:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:09:06: #1 tags after filtering in treatment: 2415711 INFO @ Fri, 16 Oct 2020 09:09:06: #1 Redundant rate of treatment: 0.15 INFO @ Fri, 16 Oct 2020 09:09:06: #1 finished! INFO @ Fri, 16 Oct 2020 09:09:06: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:09:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:09:06: #2 number of paired peaks: 46 WARNING @ Fri, 16 Oct 2020 09:09:06: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:09:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:09:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:09:10: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:09:10: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:09:15: 1000000 INFO @ Fri, 16 Oct 2020 09:09:19: 2000000 INFO @ Fri, 16 Oct 2020 09:09:23: 3000000 INFO @ Fri, 16 Oct 2020 09:09:27: 4000000 INFO @ Fri, 16 Oct 2020 09:09:31: 5000000 INFO @ Fri, 16 Oct 2020 09:09:35: 6000000 INFO @ Fri, 16 Oct 2020 09:09:35: #1 tag size is determined as 38 bps INFO @ Fri, 16 Oct 2020 09:09:35: #1 tag size = 38 INFO @ Fri, 16 Oct 2020 09:09:35: #1 total tags in treatment: 2847267 INFO @ Fri, 16 Oct 2020 09:09:35: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:09:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:09:35: #1 tags after filtering in treatment: 2415711 INFO @ Fri, 16 Oct 2020 09:09:35: #1 Redundant rate of treatment: 0.15 INFO @ Fri, 16 Oct 2020 09:09:35: #1 finished! INFO @ Fri, 16 Oct 2020 09:09:35: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:09:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:09:35: #2 number of paired peaks: 46 WARNING @ Fri, 16 Oct 2020 09:09:35: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:09:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:09:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:09:40: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:09:40: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:09:44: 1000000 INFO @ Fri, 16 Oct 2020 09:09:48: 2000000 INFO @ Fri, 16 Oct 2020 09:09:52: 3000000 INFO @ Fri, 16 Oct 2020 09:09:56: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:10:01: 5000000 INFO @ Fri, 16 Oct 2020 09:10:05: 6000000 INFO @ Fri, 16 Oct 2020 09:10:05: #1 tag size is determined as 38 bps INFO @ Fri, 16 Oct 2020 09:10:05: #1 tag size = 38 INFO @ Fri, 16 Oct 2020 09:10:05: #1 total tags in treatment: 2847267 INFO @ Fri, 16 Oct 2020 09:10:05: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:10:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:10:05: #1 tags after filtering in treatment: 2415711 INFO @ Fri, 16 Oct 2020 09:10:05: #1 Redundant rate of treatment: 0.15 INFO @ Fri, 16 Oct 2020 09:10:05: #1 finished! INFO @ Fri, 16 Oct 2020 09:10:05: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:10:05: #2 number of paired peaks: 46 WARNING @ Fri, 16 Oct 2020 09:10:05: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:10:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8754199/SRX8754199.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。