Job ID = 8184716 SRX = SRX8713424 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13605878 spots for SRR12202557/SRR12202557.sra Written 13605878 spots for SRR12202557/SRR12202557.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:55 13605878 reads; of these: 13605878 (100.00%) were unpaired; of these: 1318176 (9.69%) aligned 0 times 10936715 (80.38%) aligned exactly 1 time 1350987 (9.93%) aligned >1 times 90.31% overall alignment rate Time searching: 00:01:55 Overall time: 00:01:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4055222 / 12287702 = 0.3300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:53: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:53: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:58: 1000000 INFO @ Mon, 10 Aug 2020 14:24:04: 2000000 INFO @ Mon, 10 Aug 2020 14:24:10: 3000000 INFO @ Mon, 10 Aug 2020 14:24:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:24:22: 5000000 INFO @ Mon, 10 Aug 2020 14:24:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:24:23: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:24:23: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:24:28: 6000000 INFO @ Mon, 10 Aug 2020 14:24:29: 1000000 INFO @ Mon, 10 Aug 2020 14:24:34: 7000000 INFO @ Mon, 10 Aug 2020 14:24:35: 2000000 INFO @ Mon, 10 Aug 2020 14:24:40: 8000000 INFO @ Mon, 10 Aug 2020 14:24:41: 3000000 INFO @ Mon, 10 Aug 2020 14:24:42: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:24:42: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:24:42: #1 total tags in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:24:42: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:24:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:24:42: #1 tags after filtering in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:24:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:24:42: #1 finished! INFO @ Mon, 10 Aug 2020 14:24:42: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:24:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:24:42: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:24:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:24:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:24:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:24:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:24:53: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:24:53: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:24:53: 5000000 INFO @ Mon, 10 Aug 2020 14:24:58: 1000000 INFO @ Mon, 10 Aug 2020 14:24:59: 6000000 INFO @ Mon, 10 Aug 2020 14:25:04: 2000000 INFO @ Mon, 10 Aug 2020 14:25:06: 7000000 INFO @ Mon, 10 Aug 2020 14:25:09: 3000000 INFO @ Mon, 10 Aug 2020 14:25:12: 8000000 INFO @ Mon, 10 Aug 2020 14:25:13: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:25:13: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:25:13: #1 total tags in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:25:13: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:25:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:25:13: #1 tags after filtering in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:25:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:25:13: #1 finished! INFO @ Mon, 10 Aug 2020 14:25:13: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:25:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:25:14: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:25:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:25:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:25:15: 4000000 INFO @ Mon, 10 Aug 2020 14:25:20: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:25:25: 6000000 INFO @ Mon, 10 Aug 2020 14:25:30: 7000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:25:36: 8000000 INFO @ Mon, 10 Aug 2020 14:25:37: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:25:37: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:25:37: #1 total tags in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:25:37: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:25:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:25:37: #1 tags after filtering in treatment: 8232480 INFO @ Mon, 10 Aug 2020 14:25:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:25:37: #1 finished! INFO @ Mon, 10 Aug 2020 14:25:37: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:25:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:25:37: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:25:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:25:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713424/SRX8713424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling