Job ID = 8184715 SRX = SRX8713423 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12668417 spots for SRR12202558/SRR12202558.sra Written 12668417 spots for SRR12202558/SRR12202558.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 12668417 reads; of these: 12668417 (100.00%) were unpaired; of these: 1139734 (9.00%) aligned 0 times 10342496 (81.64%) aligned exactly 1 time 1186187 (9.36%) aligned >1 times 91.00% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3605722 / 11528683 = 0.3128 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:56: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:56: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:01: 1000000 INFO @ Mon, 10 Aug 2020 14:23:06: 2000000 INFO @ Mon, 10 Aug 2020 14:23:12: 3000000 INFO @ Mon, 10 Aug 2020 14:23:17: 4000000 INFO @ Mon, 10 Aug 2020 14:23:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:26: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:26: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:28: 6000000 INFO @ Mon, 10 Aug 2020 14:23:32: 1000000 INFO @ Mon, 10 Aug 2020 14:23:34: 7000000 INFO @ Mon, 10 Aug 2020 14:23:38: 2000000 INFO @ Mon, 10 Aug 2020 14:23:40: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:23:40: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:23:40: #1 total tags in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:23:40: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:40: #1 tags after filtering in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:23:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:40: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:40: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:40: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:23:44: 3000000 INFO @ Mon, 10 Aug 2020 14:23:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:55: 5000000 INFO @ Mon, 10 Aug 2020 14:23:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:56: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:56: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:24:01: 6000000 INFO @ Mon, 10 Aug 2020 14:24:02: 1000000 INFO @ Mon, 10 Aug 2020 14:24:08: 7000000 INFO @ Mon, 10 Aug 2020 14:24:08: 2000000 INFO @ Mon, 10 Aug 2020 14:24:13: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:24:13: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:24:13: #1 total tags in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:24:13: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:24:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:24:13: #1 tags after filtering in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:24:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:24:13: #1 finished! INFO @ Mon, 10 Aug 2020 14:24:13: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:24:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:24:14: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:24:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:24:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:24:14: 3000000 INFO @ Mon, 10 Aug 2020 14:24:20: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:24:26: 5000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:24:31: 6000000 INFO @ Mon, 10 Aug 2020 14:24:37: 7000000 INFO @ Mon, 10 Aug 2020 14:24:42: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:24:42: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:24:42: #1 total tags in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:24:42: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:24:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:24:42: #1 tags after filtering in treatment: 7922961 INFO @ Mon, 10 Aug 2020 14:24:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:24:42: #1 finished! INFO @ Mon, 10 Aug 2020 14:24:42: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:24:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:24:43: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:24:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:24:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713423/SRX8713423.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling