Job ID = 8184713 SRX = SRX8713421 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11618182 spots for SRR12202560/SRR12202560.sra Written 11618182 spots for SRR12202560/SRR12202560.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:47 11618182 reads; of these: 11618182 (100.00%) were unpaired; of these: 1082822 (9.32%) aligned 0 times 9516760 (81.91%) aligned exactly 1 time 1018600 (8.77%) aligned >1 times 90.68% overall alignment rate Time searching: 00:01:47 Overall time: 00:01:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3251012 / 10535360 = 0.3086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:12: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:12: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:17: 1000000 INFO @ Mon, 10 Aug 2020 14:22:23: 2000000 INFO @ Mon, 10 Aug 2020 14:22:28: 3000000 INFO @ Mon, 10 Aug 2020 14:22:33: 4000000 INFO @ Mon, 10 Aug 2020 14:22:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:42: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:42: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:44: 6000000 INFO @ Mon, 10 Aug 2020 14:22:48: 1000000 INFO @ Mon, 10 Aug 2020 14:22:50: 7000000 INFO @ Mon, 10 Aug 2020 14:22:52: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:22:52: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:22:52: #1 total tags in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:22:52: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:22:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:22:52: #1 tags after filtering in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:22:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:22:52: #1 finished! INFO @ Mon, 10 Aug 2020 14:22:52: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:22:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:22:53: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:22:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:22:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:22:54: 2000000 INFO @ Mon, 10 Aug 2020 14:22:59: 3000000 INFO @ Mon, 10 Aug 2020 14:23:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:10: 5000000 INFO @ Mon, 10 Aug 2020 14:23:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:12: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:12: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:16: 6000000 INFO @ Mon, 10 Aug 2020 14:23:18: 1000000 INFO @ Mon, 10 Aug 2020 14:23:22: 7000000 INFO @ Mon, 10 Aug 2020 14:23:24: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:23:24: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:23:24: #1 total tags in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:23:24: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:24: #1 tags after filtering in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:23:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:24: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:24: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:24: 2000000 INFO @ Mon, 10 Aug 2020 14:23:24: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:23:30: 3000000 INFO @ Mon, 10 Aug 2020 14:23:35: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:23:40: 5000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:23:46: 6000000 INFO @ Mon, 10 Aug 2020 14:23:51: 7000000 INFO @ Mon, 10 Aug 2020 14:23:53: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:23:53: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:23:53: #1 total tags in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:23:53: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:53: #1 tags after filtering in treatment: 7284348 INFO @ Mon, 10 Aug 2020 14:23:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:53: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:53: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:53: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713421/SRX8713421.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling