Job ID = 8184711 SRX = SRX8713419 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9297268 spots for SRR12202562/SRR12202562.sra Written 9297268 spots for SRR12202562/SRR12202562.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:59 9297268 reads; of these: 9297268 (100.00%) were unpaired; of these: 1244023 (13.38%) aligned 0 times 7198214 (77.42%) aligned exactly 1 time 855031 (9.20%) aligned >1 times 86.62% overall alignment rate Time searching: 00:00:59 Overall time: 00:00:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2134897 / 8053245 = 0.2651 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:20:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:20:25: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:20:25: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:20:30: 1000000 INFO @ Mon, 10 Aug 2020 14:20:35: 2000000 INFO @ Mon, 10 Aug 2020 14:20:40: 3000000 INFO @ Mon, 10 Aug 2020 14:20:45: 4000000 INFO @ Mon, 10 Aug 2020 14:20:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:20:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:20:55: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:20:55: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:20:55: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:20:55: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:20:55: #1 total tags in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:20:55: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:20:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:20:55: #1 tags after filtering in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:20:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:20:55: #1 finished! INFO @ Mon, 10 Aug 2020 14:20:55: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:20:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:20:55: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:20:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:20:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:21:00: 1000000 INFO @ Mon, 10 Aug 2020 14:21:06: 2000000 INFO @ Mon, 10 Aug 2020 14:21:11: 3000000 INFO @ Mon, 10 Aug 2020 14:21:17: 4000000 INFO @ Mon, 10 Aug 2020 14:21:22: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:21:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:21:25: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:21:25: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:21:27: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:21:27: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:21:27: #1 total tags in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:21:27: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:21:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:21:27: #1 tags after filtering in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:21:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:21:27: #1 finished! INFO @ Mon, 10 Aug 2020 14:21:27: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:21:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:21:27: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:21:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:21:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:21:30: 1000000 INFO @ Mon, 10 Aug 2020 14:21:36: 2000000 INFO @ Mon, 10 Aug 2020 14:21:41: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:21:47: 4000000 INFO @ Mon, 10 Aug 2020 14:21:52: 5000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:21:57: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:21:57: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:21:57: #1 total tags in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:21:57: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:21:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:21:57: #1 tags after filtering in treatment: 5918348 INFO @ Mon, 10 Aug 2020 14:21:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:21:57: #1 finished! INFO @ Mon, 10 Aug 2020 14:21:57: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:21:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:21:57: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:21:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:21:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713419/SRX8713419.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling