Job ID = 8184710 SRX = SRX8713418 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13811924 spots for SRR12202563/SRR12202563.sra Written 13811924 spots for SRR12202563/SRR12202563.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 13811924 reads; of these: 13811924 (100.00%) were unpaired; of these: 1748034 (12.66%) aligned 0 times 10861837 (78.64%) aligned exactly 1 time 1202053 (8.70%) aligned >1 times 87.34% overall alignment rate Time searching: 00:01:40 Overall time: 00:01:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4437465 / 12063890 = 0.3678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:21:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:21:31: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:21:31: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:21:36: 1000000 INFO @ Mon, 10 Aug 2020 14:21:42: 2000000 INFO @ Mon, 10 Aug 2020 14:21:47: 3000000 INFO @ Mon, 10 Aug 2020 14:21:52: 4000000 INFO @ Mon, 10 Aug 2020 14:21:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:01: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:01: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:03: 6000000 INFO @ Mon, 10 Aug 2020 14:22:06: 1000000 INFO @ Mon, 10 Aug 2020 14:22:08: 7000000 INFO @ Mon, 10 Aug 2020 14:22:10: 2000000 INFO @ Mon, 10 Aug 2020 14:22:12: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:22:12: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:22:12: #1 total tags in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:22:12: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:22:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:22:12: #1 tags after filtering in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:22:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:22:12: #1 finished! INFO @ Mon, 10 Aug 2020 14:22:12: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:22:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:22:12: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:22:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:22:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:22:15: 3000000 INFO @ Mon, 10 Aug 2020 14:22:20: 4000000 INFO @ Mon, 10 Aug 2020 14:22:25: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:29: 6000000 INFO @ Mon, 10 Aug 2020 14:22:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:31: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:31: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:34: 7000000 INFO @ Mon, 10 Aug 2020 14:22:36: 1000000 INFO @ Mon, 10 Aug 2020 14:22:37: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:22:37: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:22:37: #1 total tags in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:22:37: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:22:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:22:37: #1 tags after filtering in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:22:37: #1 finished! INFO @ Mon, 10 Aug 2020 14:22:37: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:22:38: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:22:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:22:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:22:40: 2000000 INFO @ Mon, 10 Aug 2020 14:22:45: 3000000 INFO @ Mon, 10 Aug 2020 14:22:50: 4000000 INFO @ Mon, 10 Aug 2020 14:22:54: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:22:59: 6000000 INFO @ Mon, 10 Aug 2020 14:23:03: 7000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:23:06: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:23:06: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:23:06: #1 total tags in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:23:06: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:06: #1 tags after filtering in treatment: 7626425 INFO @ Mon, 10 Aug 2020 14:23:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:06: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:06: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:07: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713418/SRX8713418.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling