Job ID = 8184709 SRX = SRX8713417 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15756796 spots for SRR12202564/SRR12202564.sra Written 15756796 spots for SRR12202564/SRR12202564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Warning: skipping read 'SRR12202564.9378 9378 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9378 9378 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12941 12941 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12941 12941 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.78229 78229 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.78229 78229 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.105039 105039 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.105039 105039 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.229251 229251 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.229251 229251 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.240978 240978 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.240978 240978 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.388562 388562 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.388562 388562 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.473140 473140 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.473140 473140 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.569413 569413 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.569413 569413 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.790403 790403 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.790403 790403 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.831914 831914 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.831914 831914 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.833933 833933 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.833933 833933 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1141604 1141604 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1141604 1141604 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1168778 1168778 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1168778 1168778 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1452316 1452316 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1452316 1452316 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1468655 1468655 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1468655 1468655 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1494276 1494276 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1494276 1494276 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1647875 1647875 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1647875 1647875 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1973774 1973774 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1973774 1973774 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.1976585 1976585 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.1976585 1976585 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2001464 2001464 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2001464 2001464 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2035247 2035247 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2035247 2035247 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2053331 2053331 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2053331 2053331 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2056048 2056048 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2056048 2056048 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2159449 2159449 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2159449 2159449 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2197480 2197480 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2197480 2197480 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2267146 2267146 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2267146 2267146 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2329903 2329903 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2329903 2329903 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2395335 2395335 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2395335 2395335 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2762872 2762872 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2762872 2762872 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2901156 2901156 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2901156 2901156 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.2984781 2984781 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.2984781 2984781 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3047800 3047800 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3047800 3047800 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3196822 3196822 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3196822 3196822 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3231208 3231208 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3231208 3231208 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3429549 3429549 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3429549 3429549 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3441562 3441562 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3441562 3441562 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3443166 3443166 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3443166 3443166 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3460240 3460240 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3460240 3460240 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3498188 3498188 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3498188 3498188 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3646252 3646252 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3646252 3646252 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3718711 3718711 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3718711 3718711 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3759216 3759216 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3759216 3759216 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3807845 3807845 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3807845 3807845 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3830389 3830389 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3830389 3830389 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3838038 3838038 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3838038 3838038 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3860870 3860870 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3860870 3860870 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3878115 3878115 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3878115 3878115 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3914805 3914805 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3914805 3914805 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.3923031 3923031 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.3923031 3923031 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4011554 4011554 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4011554 4011554 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4117329 4117329 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4117329 4117329 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4132954 4132954 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4132954 4132954 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4182219 4182219 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4182219 4182219 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4266055 4266055 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4266055 4266055 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4268676 4268676 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4268676 4268676 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4304223 4304223 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4304223 4304223 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4323910 4323910 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4323910 4323910 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4329926 4329926 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4329926 4329926 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4387208 4387208 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4387208 4387208 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4504358 4504358 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4504358 4504358 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4593510 4593510 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4593510 4593510 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4604837 4604837 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4604837 4604837 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4674573 4674573 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4674573 4674573 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4691689 4691689 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4691689 4691689 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4695304 4695304 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4695304 4695304 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4756509 4756509 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4756509 4756509 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4802677 4802677 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4802677 4802677 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4940962 4940962 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4940962 4940962 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.4972299 4972299 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.4972299 4972299 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5050248 5050248 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5050248 5050248 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5085164 5085164 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5085164 5085164 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5097584 5097584 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5097584 5097584 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5140991 5140991 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5140991 5140991 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5168483 5168483 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5168483 5168483 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5175014 5175014 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5175014 5175014 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5186534 5186534 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5186534 5186534 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5187371 5187371 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5187371 5187371 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5237591 5237591 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5237591 5237591 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5305818 5305818 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5305818 5305818 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5315307 5315307 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5315307 5315307 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5342196 5342196 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5342196 5342196 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5366457 5366457 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5366457 5366457 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5401352 5401352 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5401352 5401352 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5558434 5558434 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5558434 5558434 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.5784410 5784410 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.5784410 5784410 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6153261 6153261 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6153261 6153261 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6239181 6239181 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6239181 6239181 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6299781 6299781 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6299781 6299781 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6389124 6389124 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6389124 6389124 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6418481 6418481 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6418481 6418481 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6536753 6536753 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6536753 6536753 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6732505 6732505 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6732505 6732505 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.6791910 6791910 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.6791910 6791910 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7120234 7120234 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7120234 7120234 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7164715 7164715 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7164715 7164715 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7302871 7302871 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7302871 7302871 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7320180 7320180 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7320180 7320180 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7439867 7439867 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7439867 7439867 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7446595 7446595 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7446595 7446595 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7573852 7573852 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7573852 7573852 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7579763 7579763 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7579763 7579763 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7601466 7601466 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7601466 7601466 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7620717 7620717 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7620717 7620717 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7730413 7730413 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7730413 7730413 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7776573 7776573 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7776573 7776573 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7778269 7778269 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7778269 7778269 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7939589 7939589 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7939589 7939589 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7945765 7945765 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7945765 7945765 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.7983074 7983074 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.7983074 7983074 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.8019303 8019303 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.8019303 8019303 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.8215864 8215864 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.8215864 8215864 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.8481226 8481226 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.8481226 8481226 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.8953730 8953730 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.8953730 8953730 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.8977717 8977717 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.8977717 8977717 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9206046 9206046 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9206046 9206046 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9217466 9217466 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9217466 9217466 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9304176 9304176 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9304176 9304176 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9497900 9497900 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9497900 9497900 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9519621 9519621 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9519621 9519621 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9532601 9532601 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9532601 9532601 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9575497 9575497 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9575497 9575497 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9576604 9576604 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9576604 9576604 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9688464 9688464 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9688464 9688464 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9738208 9738208 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9738208 9738208 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9742713 9742713 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9742713 9742713 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9777873 9777873 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9777873 9777873 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.9871388 9871388 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.9871388 9871388 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10016130 10016130 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10016130 10016130 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10081289 10081289 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10081289 10081289 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10087433 10087433 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10087433 10087433 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10188647 10188647 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10188647 10188647 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10214292 10214292 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10214292 10214292 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10232422 10232422 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10232422 10232422 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10262865 10262865 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10262865 10262865 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10272642 10272642 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10272642 10272642 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10284771 10284771 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10284771 10284771 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10385966 10385966 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10385966 10385966 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10401550 10401550 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10401550 10401550 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10429667 10429667 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10429667 10429667 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10509082 10509082 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10509082 10509082 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10531887 10531887 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10531887 10531887 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10655821 10655821 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10655821 10655821 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10677748 10677748 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10677748 10677748 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10767878 10767878 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10767878 10767878 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10819032 10819032 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10819032 10819032 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.10975970 10975970 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.10975970 10975970 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11068302 11068302 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11068302 11068302 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11191747 11191747 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11191747 11191747 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11254538 11254538 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11254538 11254538 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11332641 11332641 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11332641 11332641 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11396318 11396318 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11396318 11396318 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11439231 11439231 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11439231 11439231 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11543662 11543662 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11543662 11543662 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11621113 11621113 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11621113 11621113 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11765316 11765316 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11765316 11765316 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11802439 11802439 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11802439 11802439 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11819268 11819268 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11819268 11819268 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11957867 11957867 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11957867 11957867 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.11999898 11999898 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.11999898 11999898 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12156031 12156031 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12156031 12156031 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12160479 12160479 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12160479 12160479 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12199132 12199132 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12199132 12199132 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12220476 12220476 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12220476 12220476 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12376277 12376277 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12376277 12376277 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12464367 12464367 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12464367 12464367 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12520982 12520982 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12520982 12520982 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12543301 12543301 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12543301 12543301 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12560405 12560405 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12560405 12560405 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12621534 12621534 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12621534 12621534 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12644136 12644136 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12644136 12644136 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12644795 12644795 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12644795 12644795 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12671293 12671293 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12671293 12671293 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12675175 12675175 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12675175 12675175 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12750100 12750100 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12750100 12750100 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12806792 12806792 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12806792 12806792 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12937137 12937137 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12937137 12937137 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12953191 12953191 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12953191 12953191 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.12955175 12955175 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.12955175 12955175 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13176917 13176917 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13176917 13176917 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13213674 13213674 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13213674 13213674 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13323080 13323080 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13323080 13323080 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13436086 13436086 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13436086 13436086 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13453488 13453488 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13453488 13453488 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13519622 13519622 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13519622 13519622 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13572098 13572098 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13572098 13572098 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13635003 13635003 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13635003 13635003 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13658787 13658787 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13658787 13658787 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13752180 13752180 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13752180 13752180 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13839157 13839157 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13839157 13839157 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13843048 13843048 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13843048 13843048 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13886884 13886884 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13886884 13886884 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.13972107 13972107 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.13972107 13972107 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14143181 14143181 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14143181 14143181 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14155755 14155755 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14155755 14155755 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14293564 14293564 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14293564 14293564 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14505672 14505672 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14505672 14505672 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14538863 14538863 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14538863 14538863 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.14672982 14672982 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.14672982 14672982 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15010387 15010387 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15010387 15010387 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15127483 15127483 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15127483 15127483 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15208787 15208787 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15208787 15208787 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15300736 15300736 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15300736 15300736 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15308385 15308385 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15308385 15308385 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15341049 15341049 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15341049 15341049 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15384920 15384920 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15384920 15384920 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15418135 15418135 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15418135 15418135 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15721033 15721033 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15721033 15721033 length=1' because it was < 2 characters long Warning: skipping read 'SRR12202564.15730316 15730316 length=1' because length (1) <= # seed mismatches (0) Warning: skipping read 'SRR12202564.15730316 15730316 length=1' because it was < 2 characters long Multiseed full-index search: 00:02:10 15756796 reads; of these: 15756796 (100.00%) were unpaired; of these: 1348750 (8.56%) aligned 0 times 12957411 (82.23%) aligned exactly 1 time 1450635 (9.21%) aligned >1 times 91.44% overall alignment rate Time searching: 00:02:10 Overall time: 00:02:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4877932 / 14408046 = 0.3386 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:36: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:36: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:42: 1000000 INFO @ Mon, 10 Aug 2020 14:22:47: 2000000 INFO @ Mon, 10 Aug 2020 14:22:52: 3000000 INFO @ Mon, 10 Aug 2020 14:22:58: 4000000 INFO @ Mon, 10 Aug 2020 14:23:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:06: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:06: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:08: 6000000 INFO @ Mon, 10 Aug 2020 14:23:11: 1000000 INFO @ Mon, 10 Aug 2020 14:23:14: 7000000 INFO @ Mon, 10 Aug 2020 14:23:16: 2000000 INFO @ Mon, 10 Aug 2020 14:23:19: 8000000 INFO @ Mon, 10 Aug 2020 14:23:21: 3000000 INFO @ Mon, 10 Aug 2020 14:23:25: 9000000 INFO @ Mon, 10 Aug 2020 14:23:26: 4000000 INFO @ Mon, 10 Aug 2020 14:23:28: #1 tag size is determined as 44 bps INFO @ Mon, 10 Aug 2020 14:23:28: #1 tag size = 44 INFO @ Mon, 10 Aug 2020 14:23:28: #1 total tags in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:23:28: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:28: #1 tags after filtering in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:23:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:28: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:28: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:28: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:23:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:23:35: 6000000 INFO @ Mon, 10 Aug 2020 14:23:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:23:36: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:23:36: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:23:39: 7000000 INFO @ Mon, 10 Aug 2020 14:23:41: 1000000 INFO @ Mon, 10 Aug 2020 14:23:44: 8000000 INFO @ Mon, 10 Aug 2020 14:23:46: 2000000 INFO @ Mon, 10 Aug 2020 14:23:49: 9000000 INFO @ Mon, 10 Aug 2020 14:23:50: 3000000 INFO @ Mon, 10 Aug 2020 14:23:52: #1 tag size is determined as 44 bps INFO @ Mon, 10 Aug 2020 14:23:52: #1 tag size = 44 INFO @ Mon, 10 Aug 2020 14:23:52: #1 total tags in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:23:52: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:52: #1 tags after filtering in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:23:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:52: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:52: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:52: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:23:55: 4000000 INFO @ Mon, 10 Aug 2020 14:24:00: 5000000 INFO @ Mon, 10 Aug 2020 14:24:05: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:24:09: 7000000 INFO @ Mon, 10 Aug 2020 14:24:14: 8000000 INFO @ Mon, 10 Aug 2020 14:24:19: 9000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:24:21: #1 tag size is determined as 44 bps INFO @ Mon, 10 Aug 2020 14:24:21: #1 tag size = 44 INFO @ Mon, 10 Aug 2020 14:24:21: #1 total tags in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:24:21: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:24:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:24:21: #1 tags after filtering in treatment: 9530114 INFO @ Mon, 10 Aug 2020 14:24:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:24:21: #1 finished! INFO @ Mon, 10 Aug 2020 14:24:21: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:24:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:24:22: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:24:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:24:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713417/SRX8713417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling