Job ID = 8184706 SRX = SRX8713415 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17399052 spots for SRR12202566/SRR12202566.sra Written 17399052 spots for SRR12202566/SRR12202566.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:05 17399052 reads; of these: 17399052 (100.00%) were unpaired; of these: 1008089 (5.79%) aligned 0 times 14220572 (81.73%) aligned exactly 1 time 2170391 (12.47%) aligned >1 times 94.21% overall alignment rate Time searching: 00:02:05 Overall time: 00:02:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6498170 / 16390963 = 0.3964 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:21:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:21:58: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:21:58: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:03: 1000000 INFO @ Mon, 10 Aug 2020 14:22:09: 2000000 INFO @ Mon, 10 Aug 2020 14:22:14: 3000000 INFO @ Mon, 10 Aug 2020 14:22:20: 4000000 INFO @ Mon, 10 Aug 2020 14:22:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:28: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:28: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:31: 6000000 INFO @ Mon, 10 Aug 2020 14:22:36: 1000000 INFO @ Mon, 10 Aug 2020 14:22:37: 7000000 INFO @ Mon, 10 Aug 2020 14:22:43: 2000000 INFO @ Mon, 10 Aug 2020 14:22:44: 8000000 INFO @ Mon, 10 Aug 2020 14:22:51: 9000000 INFO @ Mon, 10 Aug 2020 14:22:51: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:22:56: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:22:56: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:22:56: #1 total tags in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:22:56: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:22:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:22:57: #1 tags after filtering in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:22:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:22:57: #1 finished! INFO @ Mon, 10 Aug 2020 14:22:57: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:22:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:22:57: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:22:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:22:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:22:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:22:58: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:22:58: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:22:58: 4000000 INFO @ Mon, 10 Aug 2020 14:23:05: 1000000 INFO @ Mon, 10 Aug 2020 14:23:06: 5000000 INFO @ Mon, 10 Aug 2020 14:23:13: 2000000 INFO @ Mon, 10 Aug 2020 14:23:14: 6000000 INFO @ Mon, 10 Aug 2020 14:23:20: 3000000 INFO @ Mon, 10 Aug 2020 14:23:21: 7000000 INFO @ Mon, 10 Aug 2020 14:23:28: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:23:29: 8000000 INFO @ Mon, 10 Aug 2020 14:23:35: 5000000 INFO @ Mon, 10 Aug 2020 14:23:37: 9000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:23:43: 6000000 INFO @ Mon, 10 Aug 2020 14:23:43: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:23:43: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:23:43: #1 total tags in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:23:43: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:23:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:23:44: #1 tags after filtering in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:23:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:23:44: #1 finished! INFO @ Mon, 10 Aug 2020 14:23:44: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:23:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:23:44: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:23:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:23:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:23:50: 7000000 INFO @ Mon, 10 Aug 2020 14:23:57: 8000000 INFO @ Mon, 10 Aug 2020 14:24:04: 9000000 INFO @ Mon, 10 Aug 2020 14:24:10: #1 tag size is determined as 50 bps INFO @ Mon, 10 Aug 2020 14:24:10: #1 tag size = 50 INFO @ Mon, 10 Aug 2020 14:24:10: #1 total tags in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:24:10: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:24:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:24:10: #1 tags after filtering in treatment: 9892793 INFO @ Mon, 10 Aug 2020 14:24:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:24:10: #1 finished! INFO @ Mon, 10 Aug 2020 14:24:10: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:24:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:24:10: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:24:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:24:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713415/SRX8713415.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling