Job ID = 8184702 SRX = SRX8713412 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22922427 spots for SRR12202569/SRR12202569.sra Written 22922427 spots for SRR12202569/SRR12202569.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:23 22922427 reads; of these: 22922427 (100.00%) were unpaired; of these: 2062241 (9.00%) aligned 0 times 18775922 (81.91%) aligned exactly 1 time 2084264 (9.09%) aligned >1 times 91.00% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9072968 / 20860186 = 0.4349 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:29:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:29:15: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:29:15: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:29:20: 1000000 INFO @ Mon, 10 Aug 2020 14:29:26: 2000000 INFO @ Mon, 10 Aug 2020 14:29:32: 3000000 INFO @ Mon, 10 Aug 2020 14:29:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:29:43: 5000000 INFO @ Mon, 10 Aug 2020 14:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:29:45: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:29:45: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:29:49: 6000000 INFO @ Mon, 10 Aug 2020 14:29:50: 1000000 INFO @ Mon, 10 Aug 2020 14:29:55: 7000000 INFO @ Mon, 10 Aug 2020 14:29:56: 2000000 INFO @ Mon, 10 Aug 2020 14:30:01: 8000000 INFO @ Mon, 10 Aug 2020 14:30:02: 3000000 INFO @ Mon, 10 Aug 2020 14:30:07: 9000000 INFO @ Mon, 10 Aug 2020 14:30:08: 4000000 INFO @ Mon, 10 Aug 2020 14:30:12: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 10 Aug 2020 14:30:14: 5000000 INFO @ Mon, 10 Aug 2020 14:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 10 Aug 2020 14:30:15: #1 read tag files... INFO @ Mon, 10 Aug 2020 14:30:15: #1 read treatment tags... INFO @ Mon, 10 Aug 2020 14:30:18: 11000000 INFO @ Mon, 10 Aug 2020 14:30:20: 6000000 INFO @ Mon, 10 Aug 2020 14:30:22: 1000000 INFO @ Mon, 10 Aug 2020 14:30:23: #1 tag size is determined as 125 bps INFO @ Mon, 10 Aug 2020 14:30:23: #1 tag size = 125 INFO @ Mon, 10 Aug 2020 14:30:23: #1 total tags in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:30:23: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:30:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:30:23: #1 tags after filtering in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:30:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:30:23: #1 finished! INFO @ Mon, 10 Aug 2020 14:30:23: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:30:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:30:24: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:30:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:30:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:30:26: 7000000 INFO @ Mon, 10 Aug 2020 14:30:29: 2000000 INFO @ Mon, 10 Aug 2020 14:30:32: 8000000 INFO @ Mon, 10 Aug 2020 14:30:36: 3000000 INFO @ Mon, 10 Aug 2020 14:30:38: 9000000 INFO @ Mon, 10 Aug 2020 14:30:43: 4000000 INFO @ Mon, 10 Aug 2020 14:30:43: 10000000 INFO @ Mon, 10 Aug 2020 14:30:49: 11000000 INFO @ Mon, 10 Aug 2020 14:30:50: 5000000 INFO @ Mon, 10 Aug 2020 14:30:54: #1 tag size is determined as 125 bps INFO @ Mon, 10 Aug 2020 14:30:54: #1 tag size = 125 INFO @ Mon, 10 Aug 2020 14:30:54: #1 total tags in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:30:54: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:30:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:30:54: #1 tags after filtering in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:30:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:30:54: #1 finished! INFO @ Mon, 10 Aug 2020 14:30:54: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:30:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:30:55: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:30:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:30:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 10 Aug 2020 14:30:57: 6000000 INFO @ Mon, 10 Aug 2020 14:31:04: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 10 Aug 2020 14:31:11: 8000000 INFO @ Mon, 10 Aug 2020 14:31:18: 9000000 BigWig に変換しました。 INFO @ Mon, 10 Aug 2020 14:31:26: 10000000 INFO @ Mon, 10 Aug 2020 14:31:33: 11000000 INFO @ Mon, 10 Aug 2020 14:31:38: #1 tag size is determined as 125 bps INFO @ Mon, 10 Aug 2020 14:31:38: #1 tag size = 125 INFO @ Mon, 10 Aug 2020 14:31:38: #1 total tags in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:31:38: #1 user defined the maximum tags... INFO @ Mon, 10 Aug 2020 14:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 10 Aug 2020 14:31:39: #1 tags after filtering in treatment: 11787218 INFO @ Mon, 10 Aug 2020 14:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 10 Aug 2020 14:31:39: #1 finished! INFO @ Mon, 10 Aug 2020 14:31:39: #2 Build Peak Model... INFO @ Mon, 10 Aug 2020 14:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 10 Aug 2020 14:31:39: #2 number of paired peaks: 0 WARNING @ Mon, 10 Aug 2020 14:31:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 10 Aug 2020 14:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8713412/SRX8713412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling