Job ID = 10224004 SRX = SRX869417 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 55597907 spots for SRR1794018/SRR1794018.sra Written 55597907 spots for SRR1794018/SRR1794018.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:06 55597907 reads; of these: 55597907 (100.00%) were unpaired; of these: 6762534 (12.16%) aligned 0 times 31756459 (57.12%) aligned exactly 1 time 17078914 (30.72%) aligned >1 times 87.84% overall alignment rate Time searching: 00:09:06 Overall time: 00:09:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 35279213 / 48835373 = 0.7224 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:27:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:27:40: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:27:40: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:27:46: 1000000 INFO @ Fri, 16 Oct 2020 09:27:52: 2000000 INFO @ Fri, 16 Oct 2020 09:27:57: 3000000 INFO @ Fri, 16 Oct 2020 09:28:03: 4000000 INFO @ Fri, 16 Oct 2020 09:28:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:28:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:28:10: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:28:10: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:28:14: 6000000 INFO @ Fri, 16 Oct 2020 09:28:17: 1000000 INFO @ Fri, 16 Oct 2020 09:28:20: 7000000 INFO @ Fri, 16 Oct 2020 09:28:24: 2000000 INFO @ Fri, 16 Oct 2020 09:28:27: 8000000 INFO @ Fri, 16 Oct 2020 09:28:30: 3000000 INFO @ Fri, 16 Oct 2020 09:28:33: 9000000 INFO @ Fri, 16 Oct 2020 09:28:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:28:39: 10000000 INFO @ Fri, 16 Oct 2020 09:28:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:28:40: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:28:40: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:28:44: 5000000 INFO @ Fri, 16 Oct 2020 09:28:45: 11000000 INFO @ Fri, 16 Oct 2020 09:28:47: 1000000 INFO @ Fri, 16 Oct 2020 09:28:50: 12000000 INFO @ Fri, 16 Oct 2020 09:28:51: 6000000 INFO @ Fri, 16 Oct 2020 09:28:53: 2000000 INFO @ Fri, 16 Oct 2020 09:28:56: 13000000 INFO @ Fri, 16 Oct 2020 09:28:57: 7000000 INFO @ Fri, 16 Oct 2020 09:28:59: #1 tag size is determined as 75 bps INFO @ Fri, 16 Oct 2020 09:28:59: #1 tag size = 75 INFO @ Fri, 16 Oct 2020 09:28:59: #1 total tags in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:28:59: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:28:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:29:00: #1 tags after filtering in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:29:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:29:00: #1 finished! INFO @ Fri, 16 Oct 2020 09:29:00: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:29:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:29:00: 3000000 INFO @ Fri, 16 Oct 2020 09:29:00: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:29:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:29:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:29:04: 8000000 INFO @ Fri, 16 Oct 2020 09:29:06: 4000000 INFO @ Fri, 16 Oct 2020 09:29:10: 9000000 INFO @ Fri, 16 Oct 2020 09:29:12: 5000000 INFO @ Fri, 16 Oct 2020 09:29:17: 10000000 INFO @ Fri, 16 Oct 2020 09:29:18: 6000000 INFO @ Fri, 16 Oct 2020 09:29:23: 11000000 INFO @ Fri, 16 Oct 2020 09:29:24: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:29:30: 8000000 INFO @ Fri, 16 Oct 2020 09:29:30: 12000000 INFO @ Fri, 16 Oct 2020 09:29:36: 9000000 INFO @ Fri, 16 Oct 2020 09:29:36: 13000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:29:40: #1 tag size is determined as 75 bps INFO @ Fri, 16 Oct 2020 09:29:40: #1 tag size = 75 INFO @ Fri, 16 Oct 2020 09:29:40: #1 total tags in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:29:40: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:29:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:29:40: #1 tags after filtering in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:29:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:29:40: #1 finished! INFO @ Fri, 16 Oct 2020 09:29:40: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:29:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:29:41: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:29:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:29:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:29:41: 10000000 INFO @ Fri, 16 Oct 2020 09:29:47: 11000000 INFO @ Fri, 16 Oct 2020 09:29:53: 12000000 INFO @ Fri, 16 Oct 2020 09:29:58: 13000000 INFO @ Fri, 16 Oct 2020 09:30:01: #1 tag size is determined as 75 bps INFO @ Fri, 16 Oct 2020 09:30:01: #1 tag size = 75 INFO @ Fri, 16 Oct 2020 09:30:01: #1 total tags in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:30:01: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:30:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:30:01: #1 tags after filtering in treatment: 13556160 INFO @ Fri, 16 Oct 2020 09:30:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:30:01: #1 finished! INFO @ Fri, 16 Oct 2020 09:30:01: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:30:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:30:02: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:30:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:30:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX869417/SRX869417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling