Job ID = 9163535 sra ファイルのダウンロード中... Completed: 510976K bytes transferred in 7 seconds (526585K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 15688854 spots for /home/okishinya/chipatlas/results/sacCer3/SRX869398/SRR1793999.sra Written 15688854 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:43 15688854 reads; of these: 15688854 (100.00%) were unpaired; of these: 274118 (1.75%) aligned 0 times 13023269 (83.01%) aligned exactly 1 time 2391467 (15.24%) aligned >1 times 98.25% overall alignment rate Time searching: 00:02:43 Overall time: 00:02:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5884349 / 15414736 = 0.3817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 10:57:59: # Command line: callpeak -t SRX869398.bam -f BAM -g 12100000 -n SRX869398.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX869398.05 # format = BAM # ChIP-seq file = ['SRX869398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:57:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:57:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:57:59: # Command line: callpeak -t SRX869398.bam -f BAM -g 12100000 -n SRX869398.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX869398.20 # format = BAM # ChIP-seq file = ['SRX869398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:57:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:57:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:57:59: # Command line: callpeak -t SRX869398.bam -f BAM -g 12100000 -n SRX869398.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX869398.10 # format = BAM # ChIP-seq file = ['SRX869398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:57:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:57:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:58:06: 1000000 INFO @ Wed, 28 Jun 2017 10:58:07: 1000000 INFO @ Wed, 28 Jun 2017 10:58:07: 1000000 INFO @ Wed, 28 Jun 2017 10:58:13: 2000000 INFO @ Wed, 28 Jun 2017 10:58:14: 2000000 INFO @ Wed, 28 Jun 2017 10:58:14: 2000000 INFO @ Wed, 28 Jun 2017 10:58:20: 3000000 INFO @ Wed, 28 Jun 2017 10:58:21: 3000000 INFO @ Wed, 28 Jun 2017 10:58:21: 3000000 INFO @ Wed, 28 Jun 2017 10:58:27: 4000000 INFO @ Wed, 28 Jun 2017 10:58:28: 4000000 INFO @ Wed, 28 Jun 2017 10:58:28: 4000000 INFO @ Wed, 28 Jun 2017 10:58:33: 5000000 INFO @ Wed, 28 Jun 2017 10:58:35: 5000000 INFO @ Wed, 28 Jun 2017 10:58:35: 5000000 INFO @ Wed, 28 Jun 2017 10:58:40: 6000000 INFO @ Wed, 28 Jun 2017 10:58:42: 6000000 INFO @ Wed, 28 Jun 2017 10:58:42: 6000000 INFO @ Wed, 28 Jun 2017 10:58:47: 7000000 INFO @ Wed, 28 Jun 2017 10:58:49: 7000000 INFO @ Wed, 28 Jun 2017 10:58:49: 7000000 INFO @ Wed, 28 Jun 2017 10:58:54: 8000000 INFO @ Wed, 28 Jun 2017 10:58:57: 8000000 INFO @ Wed, 28 Jun 2017 10:58:57: 8000000 INFO @ Wed, 28 Jun 2017 10:59:01: 9000000 INFO @ Wed, 28 Jun 2017 10:59:04: 9000000 INFO @ Wed, 28 Jun 2017 10:59:04: 9000000 INFO @ Wed, 28 Jun 2017 10:59:05: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:05: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:05: #1 total tags in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:05: #1 tags after filtering in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:05: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:06: Process for pairing-model is terminated! cat: SRX869398.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869398.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:59:08: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:08: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:08: #1 total tags in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:08: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:08: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:08: #1 total tags in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:08: #1 tags after filtering in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:08: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:08: #1 tags after filtering in treatment: 9530387 INFO @ Wed, 28 Jun 2017 10:59:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:08: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:08: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:08: Process for pairing-model is terminated! cat: SRX869398.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869398.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:59:08: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:08: Process for pairing-model is terminated! cat: SRX869398.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869398.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869398.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。