Job ID = 7119780 SRX = SRX8641899 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:30:05 prefetch.2.10.7: 1) Downloading 'SRR12120432'... 2020-07-22T07:30:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:31:17 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:31:17 prefetch.2.10.7: 'SRR12120432' is valid 2020-07-22T07:31:17 prefetch.2.10.7: 1) 'SRR12120432' was downloaded successfully 2020-07-22T07:31:17 prefetch.2.10.7: 'SRR12120432' has 0 unresolved dependencies Read 11646365 spots for SRR12120432/SRR12120432.sra Written 11646365 spots for SRR12120432/SRR12120432.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 11646365 reads; of these: 11646365 (100.00%) were unpaired; of these: 3809759 (32.71%) aligned 0 times 6954297 (59.71%) aligned exactly 1 time 882309 (7.58%) aligned >1 times 67.29% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4281557 / 7836606 = 0.5464 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:36:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:36:59: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:36:59: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:37:06: 1000000 INFO @ Wed, 22 Jul 2020 16:37:13: 2000000 INFO @ Wed, 22 Jul 2020 16:37:20: 3000000 INFO @ Wed, 22 Jul 2020 16:37:24: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:37:24: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:37:24: #1 total tags in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:37:24: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:37:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:37:24: #1 tags after filtering in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:37:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:37:24: #1 finished! INFO @ Wed, 22 Jul 2020 16:37:24: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:37:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:37:24: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:37:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:37:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:37:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:37:29: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:37:29: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:37:35: 1000000 INFO @ Wed, 22 Jul 2020 16:37:42: 2000000 INFO @ Wed, 22 Jul 2020 16:37:49: 3000000 INFO @ Wed, 22 Jul 2020 16:37:53: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:37:53: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:37:53: #1 total tags in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:37:53: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:37:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:37:53: #1 tags after filtering in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:37:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:37:53: #1 finished! INFO @ Wed, 22 Jul 2020 16:37:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:37:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:37:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:37:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:37:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:37:59: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:37:59: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:38:06: 1000000 INFO @ Wed, 22 Jul 2020 16:38:13: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:38:20: 3000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:38:24: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:38:24: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:38:24: #1 total tags in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:38:24: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:38:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:38:24: #1 tags after filtering in treatment: 3555049 INFO @ Wed, 22 Jul 2020 16:38:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:38:24: #1 finished! INFO @ Wed, 22 Jul 2020 16:38:24: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:38:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:38:24: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:38:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:38:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641899/SRX8641899.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling