Job ID = 7119649 SRX = SRX8641891 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:28:12 prefetch.2.10.7: 1) Downloading 'SRR12120424'... 2020-07-22T07:28:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:28:54 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:28:54 prefetch.2.10.7: 'SRR12120424' is valid 2020-07-22T07:28:54 prefetch.2.10.7: 1) 'SRR12120424' was downloaded successfully 2020-07-22T07:28:54 prefetch.2.10.7: 'SRR12120424' has 0 unresolved dependencies Read 4876763 spots for SRR12120424/SRR12120424.sra Written 4876763 spots for SRR12120424/SRR12120424.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 4876763 reads; of these: 4876763 (100.00%) were unpaired; of these: 1171115 (24.01%) aligned 0 times 3104290 (63.65%) aligned exactly 1 time 601358 (12.33%) aligned >1 times 75.99% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1205402 / 3705648 = 0.3253 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:31:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:31:43: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:31:43: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:31:48: 1000000 INFO @ Wed, 22 Jul 2020 16:31:54: 2000000 INFO @ Wed, 22 Jul 2020 16:31:56: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:31:56: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:31:56: #1 total tags in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:31:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:31:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:31:56: #1 tags after filtering in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:31:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:31:56: #1 finished! INFO @ Wed, 22 Jul 2020 16:31:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:31:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:31:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:31:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:31:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:32:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:32:13: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:32:13: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:32:19: 1000000 INFO @ Wed, 22 Jul 2020 16:32:25: 2000000 INFO @ Wed, 22 Jul 2020 16:32:28: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:32:28: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:32:28: #1 total tags in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:32:28: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:32:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:32:28: #1 tags after filtering in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:32:28: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:32:28: #1 finished! INFO @ Wed, 22 Jul 2020 16:32:28: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:32:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:32:28: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:32:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:32:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:32:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:32:43: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:32:43: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:32:49: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:32:55: 2000000 INFO @ Wed, 22 Jul 2020 16:32:58: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:32:58: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:32:58: #1 total tags in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:32:58: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:32:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:32:58: #1 tags after filtering in treatment: 2500246 INFO @ Wed, 22 Jul 2020 16:32:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:32:58: #1 finished! INFO @ Wed, 22 Jul 2020 16:32:58: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:32:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:32:58: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:32:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:32:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641891/SRX8641891.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。