Job ID = 7118808 SRX = SRX8641882 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:26:03 prefetch.2.10.7: 1) Downloading 'SRR12120415'... 2020-07-22T07:26:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:27:14 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:27:14 prefetch.2.10.7: 'SRR12120415' is valid 2020-07-22T07:27:14 prefetch.2.10.7: 1) 'SRR12120415' was downloaded successfully 2020-07-22T07:27:14 prefetch.2.10.7: 'SRR12120415' has 0 unresolved dependencies Read 10015475 spots for SRR12120415/SRR12120415.sra Written 10015475 spots for SRR12120415/SRR12120415.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:19 10015475 reads; of these: 10015475 (100.00%) were unpaired; of these: 2425474 (24.22%) aligned 0 times 6743454 (67.33%) aligned exactly 1 time 846547 (8.45%) aligned >1 times 75.78% overall alignment rate Time searching: 00:02:19 Overall time: 00:02:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3831729 / 7590001 = 0.5048 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:32:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:32:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:32:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:32:19: 1000000 INFO @ Wed, 22 Jul 2020 16:32:27: 2000000 INFO @ Wed, 22 Jul 2020 16:32:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:32:41: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:32:41: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:32:41: #1 total tags in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:32:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:32:41: #1 tags after filtering in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:32:41: #1 finished! INFO @ Wed, 22 Jul 2020 16:32:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:32:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:32:41: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:32:41: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:32:41: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:32:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:32:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:32:47: 1000000 INFO @ Wed, 22 Jul 2020 16:32:54: 2000000 INFO @ Wed, 22 Jul 2020 16:33:00: 3000000 INFO @ Wed, 22 Jul 2020 16:33:05: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:33:05: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:33:05: #1 total tags in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:33:05: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:33:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:33:05: #1 tags after filtering in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:33:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:33:05: #1 finished! INFO @ Wed, 22 Jul 2020 16:33:05: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:33:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:33:05: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:33:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:33:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:33:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:33:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:33:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:33:17: 1000000 INFO @ Wed, 22 Jul 2020 16:33:24: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:33:30: 3000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:33:35: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:33:35: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:33:35: #1 total tags in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:33:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:33:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:33:35: #1 tags after filtering in treatment: 3758272 INFO @ Wed, 22 Jul 2020 16:33:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:33:35: #1 finished! INFO @ Wed, 22 Jul 2020 16:33:35: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:33:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:33:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:33:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:33:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641882/SRX8641882.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling