Job ID = 7118782 SRX = SRX8641881 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:26:03 prefetch.2.10.7: 1) Downloading 'SRR12120414'... 2020-07-22T07:26:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:28:20 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:28:20 prefetch.2.10.7: 1) 'SRR12120414' was downloaded successfully 2020-07-22T07:28:20 prefetch.2.10.7: 'SRR12120414' has 0 unresolved dependencies Read 16994235 spots for SRR12120414/SRR12120414.sra Written 16994235 spots for SRR12120414/SRR12120414.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:17 16994235 reads; of these: 16994235 (100.00%) were unpaired; of these: 1513767 (8.91%) aligned 0 times 13831577 (81.39%) aligned exactly 1 time 1648891 (9.70%) aligned >1 times 91.09% overall alignment rate Time searching: 00:05:17 Overall time: 00:05:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10644468 / 15480468 = 0.6876 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:39:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:39:19: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:39:19: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:39:26: 1000000 INFO @ Wed, 22 Jul 2020 16:39:34: 2000000 INFO @ Wed, 22 Jul 2020 16:39:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:39:48: 4000000 INFO @ Wed, 22 Jul 2020 16:39:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:39:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:39:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:39:56: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:39:56: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:39:56: #1 total tags in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:39:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:39:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:39:56: #1 tags after filtering in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:39:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:39:56: #1 finished! INFO @ Wed, 22 Jul 2020 16:39:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:39:56: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:39:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:39:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:39:58: 1000000 INFO @ Wed, 22 Jul 2020 16:40:06: 2000000 INFO @ Wed, 22 Jul 2020 16:40:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:40:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:40:19: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:40:19: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:40:21: 4000000 INFO @ Wed, 22 Jul 2020 16:40:27: 1000000 INFO @ Wed, 22 Jul 2020 16:40:28: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:40:28: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:40:28: #1 total tags in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:40:28: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:40:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:40:28: #1 tags after filtering in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:40:28: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:40:28: #1 finished! INFO @ Wed, 22 Jul 2020 16:40:28: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:40:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:40:29: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:40:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:40:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:40:35: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:40:43: 3000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:40:51: 4000000 INFO @ Wed, 22 Jul 2020 16:40:57: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:40:57: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:40:57: #1 total tags in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:40:57: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:40:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:40:57: #1 tags after filtering in treatment: 4836000 INFO @ Wed, 22 Jul 2020 16:40:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:40:57: #1 finished! INFO @ Wed, 22 Jul 2020 16:40:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:40:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:40:58: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:40:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:40:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641881/SRX8641881.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling