Job ID = 7118685 SRX = SRX8641878 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:25:18 prefetch.2.10.7: 1) Downloading 'SRR12120411'... 2020-07-22T07:25:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:26:04 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:26:05 prefetch.2.10.7: 'SRR12120411' is valid 2020-07-22T07:26:05 prefetch.2.10.7: 1) 'SRR12120411' was downloaded successfully 2020-07-22T07:26:05 prefetch.2.10.7: 'SRR12120411' has 0 unresolved dependencies Read 9000766 spots for SRR12120411/SRR12120411.sra Written 9000766 spots for SRR12120411/SRR12120411.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:11 9000766 reads; of these: 9000766 (100.00%) were unpaired; of these: 2280287 (25.33%) aligned 0 times 6017317 (66.85%) aligned exactly 1 time 703162 (7.81%) aligned >1 times 74.67% overall alignment rate Time searching: 00:02:11 Overall time: 00:02:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3177993 / 6720479 = 0.4729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:30:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:30:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:30:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:30:52: 1000000 INFO @ Wed, 22 Jul 2020 16:30:57: 2000000 INFO @ Wed, 22 Jul 2020 16:31:03: 3000000 INFO @ Wed, 22 Jul 2020 16:31:06: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:31:06: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:31:06: #1 total tags in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:31:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:31:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:31:06: #1 tags after filtering in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:31:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:31:06: #1 finished! INFO @ Wed, 22 Jul 2020 16:31:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:31:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:31:06: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:31:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:31:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:31:16: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:31:16: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:31:21: 1000000 INFO @ Wed, 22 Jul 2020 16:31:27: 2000000 INFO @ Wed, 22 Jul 2020 16:31:32: 3000000 INFO @ Wed, 22 Jul 2020 16:31:35: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:31:35: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:31:35: #1 total tags in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:31:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:31:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:31:35: #1 tags after filtering in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:31:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:31:35: #1 finished! INFO @ Wed, 22 Jul 2020 16:31:35: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:31:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:31:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:31:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:31:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:31:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:31:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:31:52: 1000000 INFO @ Wed, 22 Jul 2020 16:31:59: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:32:05: 3000000 INFO @ Wed, 22 Jul 2020 16:32:09: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:32:09: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:32:09: #1 total tags in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:32:09: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:32:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:32:09: #1 tags after filtering in treatment: 3542486 INFO @ Wed, 22 Jul 2020 16:32:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:32:09: #1 finished! INFO @ Wed, 22 Jul 2020 16:32:09: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:32:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:32:09: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:32:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:32:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641878/SRX8641878.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。