Job ID = 7118551 SRX = SRX8641873 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:24:18 prefetch.2.10.7: 1) Downloading 'SRR12120406'... 2020-07-22T07:24:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:25:01 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:25:02 prefetch.2.10.7: 'SRR12120406' is valid 2020-07-22T07:25:02 prefetch.2.10.7: 1) 'SRR12120406' was downloaded successfully 2020-07-22T07:25:02 prefetch.2.10.7: 'SRR12120406' has 0 unresolved dependencies Read 8968296 spots for SRR12120406/SRR12120406.sra Written 8968296 spots for SRR12120406/SRR12120406.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:32 8968296 reads; of these: 8968296 (100.00%) were unpaired; of these: 2110210 (23.53%) aligned 0 times 6056682 (67.53%) aligned exactly 1 time 801404 (8.94%) aligned >1 times 76.47% overall alignment rate Time searching: 00:02:32 Overall time: 00:02:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3282089 / 6858086 = 0.4786 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:30:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:30:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:30:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:30:16: 1000000 INFO @ Wed, 22 Jul 2020 16:30:22: 2000000 INFO @ Wed, 22 Jul 2020 16:30:28: 3000000 INFO @ Wed, 22 Jul 2020 16:30:31: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:30:31: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:30:31: #1 total tags in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:30:31: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:30:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:30:31: #1 tags after filtering in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:30:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:30:31: #1 finished! INFO @ Wed, 22 Jul 2020 16:30:31: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:30:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:30:31: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:30:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:30:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:30:40: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:30:40: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:30:46: 1000000 INFO @ Wed, 22 Jul 2020 16:30:52: 2000000 INFO @ Wed, 22 Jul 2020 16:30:57: 3000000 INFO @ Wed, 22 Jul 2020 16:31:00: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:31:00: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:31:00: #1 total tags in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:31:00: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:31:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:31:00: #1 tags after filtering in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:31:00: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:31:00: #1 finished! INFO @ Wed, 22 Jul 2020 16:31:00: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:31:00: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:31:01: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:31:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:31:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:31:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:31:10: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:31:10: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:31:16: 1000000 INFO @ Wed, 22 Jul 2020 16:31:22: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:31:27: 3000000 INFO @ Wed, 22 Jul 2020 16:31:31: #1 tag size is determined as 74 bps INFO @ Wed, 22 Jul 2020 16:31:31: #1 tag size = 74 INFO @ Wed, 22 Jul 2020 16:31:31: #1 total tags in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:31:31: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:31:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:31:31: #1 tags after filtering in treatment: 3575997 INFO @ Wed, 22 Jul 2020 16:31:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:31:31: #1 finished! INFO @ Wed, 22 Jul 2020 16:31:31: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:31:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:31:31: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:31:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:31:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641873/SRX8641873.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。