Job ID = 7118331 SRX = SRX8641841 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-22T07:15:49 prefetch.2.10.7: 1) Downloading 'SRR12120449'... 2020-07-22T07:15:49 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:16:25 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:16:25 prefetch.2.10.7: 'SRR12120449' is valid 2020-07-22T07:16:25 prefetch.2.10.7: 1) 'SRR12120449' was downloaded successfully 2020-07-22T07:16:25 prefetch.2.10.7: 'SRR12120449' has 0 unresolved dependencies Read 4088745 spots for SRR12120449/SRR12120449.sra Written 4088745 spots for SRR12120449/SRR12120449.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:06 4088745 reads; of these: 4088745 (100.00%) were unpaired; of these: 830884 (20.32%) aligned 0 times 2921165 (71.44%) aligned exactly 1 time 336696 (8.23%) aligned >1 times 79.68% overall alignment rate Time searching: 00:01:06 Overall time: 00:01:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 963544 / 3257861 = 0.2958 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:19:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:19:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:19:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:19:55: 1000000 INFO @ Wed, 22 Jul 2020 16:20:01: 2000000 INFO @ Wed, 22 Jul 2020 16:20:03: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:20:03: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:20:03: #1 total tags in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:20:03: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:20:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:20:03: #1 tags after filtering in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:20:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:20:03: #1 finished! INFO @ Wed, 22 Jul 2020 16:20:03: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:20:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:20:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:20:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:20:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:20:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:20:19: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:20:19: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:20:25: 1000000 INFO @ Wed, 22 Jul 2020 16:20:31: 2000000 INFO @ Wed, 22 Jul 2020 16:20:33: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:20:33: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:20:33: #1 total tags in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:20:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:20:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:20:33: #1 tags after filtering in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:20:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:20:33: #1 finished! INFO @ Wed, 22 Jul 2020 16:20:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:20:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:20:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:20:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:20:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:20:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:20:49: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:20:49: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:20:55: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:21:01: 2000000 INFO @ Wed, 22 Jul 2020 16:21:03: #1 tag size is determined as 90 bps INFO @ Wed, 22 Jul 2020 16:21:03: #1 tag size = 90 INFO @ Wed, 22 Jul 2020 16:21:03: #1 total tags in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:21:03: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:21:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:21:03: #1 tags after filtering in treatment: 2294317 INFO @ Wed, 22 Jul 2020 16:21:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 16:21:03: #1 finished! INFO @ Wed, 22 Jul 2020 16:21:03: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:21:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:21:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:21:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:21:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8641841/SRX8641841.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。