Job ID = 7118266 SRX = SRX8639587 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T07:12:52 prefetch.2.10.7: 1) Downloading 'SRR12117025'... 2020-07-22T07:12:52 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T07:15:01 prefetch.2.10.7: HTTPS download succeed 2020-07-22T07:15:01 prefetch.2.10.7: 1) 'SRR12117025' was downloaded successfully 2020-07-22T07:15:01 prefetch.2.10.7: 'SRR12117025' has 0 unresolved dependencies Read 31771729 spots for SRR12117025/SRR12117025.sra Written 31771729 spots for SRR12117025/SRR12117025.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:34 31771729 reads; of these: 31771729 (100.00%) were paired; of these: 4235676 (13.33%) aligned concordantly 0 times 24238233 (76.29%) aligned concordantly exactly 1 time 3297820 (10.38%) aligned concordantly >1 times ---- 4235676 pairs aligned concordantly 0 times; of these: 360041 (8.50%) aligned discordantly 1 time ---- 3875635 pairs aligned 0 times concordantly or discordantly; of these: 7751270 mates make up the pairs; of these: 7309733 (94.30%) aligned 0 times 308311 (3.98%) aligned exactly 1 time 133226 (1.72%) aligned >1 times 88.50% overall alignment rate Time searching: 00:15:34 Overall time: 00:15:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 651992 / 27863200 = 0.0234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:44:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:44:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:44:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:44:30: 1000000 INFO @ Wed, 22 Jul 2020 16:44:36: 2000000 INFO @ Wed, 22 Jul 2020 16:44:42: 3000000 INFO @ Wed, 22 Jul 2020 16:44:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:44:53: 5000000 INFO @ Wed, 22 Jul 2020 16:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:44:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:44:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:45:00: 6000000 INFO @ Wed, 22 Jul 2020 16:45:01: 1000000 INFO @ Wed, 22 Jul 2020 16:45:07: 7000000 INFO @ Wed, 22 Jul 2020 16:45:09: 2000000 INFO @ Wed, 22 Jul 2020 16:45:14: 8000000 INFO @ Wed, 22 Jul 2020 16:45:15: 3000000 INFO @ Wed, 22 Jul 2020 16:45:22: 9000000 INFO @ Wed, 22 Jul 2020 16:45:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:45:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:45:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:45:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:45:29: 5000000 INFO @ Wed, 22 Jul 2020 16:45:29: 10000000 INFO @ Wed, 22 Jul 2020 16:45:32: 1000000 INFO @ Wed, 22 Jul 2020 16:45:36: 6000000 INFO @ Wed, 22 Jul 2020 16:45:37: 11000000 INFO @ Wed, 22 Jul 2020 16:45:39: 2000000 INFO @ Wed, 22 Jul 2020 16:45:43: 7000000 INFO @ Wed, 22 Jul 2020 16:45:44: 12000000 INFO @ Wed, 22 Jul 2020 16:45:46: 3000000 INFO @ Wed, 22 Jul 2020 16:45:49: 8000000 INFO @ Wed, 22 Jul 2020 16:45:51: 13000000 INFO @ Wed, 22 Jul 2020 16:45:53: 4000000 INFO @ Wed, 22 Jul 2020 16:45:56: 9000000 INFO @ Wed, 22 Jul 2020 16:45:59: 14000000 INFO @ Wed, 22 Jul 2020 16:46:01: 5000000 INFO @ Wed, 22 Jul 2020 16:46:02: 10000000 INFO @ Wed, 22 Jul 2020 16:46:06: 15000000 INFO @ Wed, 22 Jul 2020 16:46:08: 6000000 INFO @ Wed, 22 Jul 2020 16:46:09: 11000000 INFO @ Wed, 22 Jul 2020 16:46:13: 16000000 INFO @ Wed, 22 Jul 2020 16:46:15: 7000000 INFO @ Wed, 22 Jul 2020 16:46:16: 12000000 INFO @ Wed, 22 Jul 2020 16:46:20: 17000000 INFO @ Wed, 22 Jul 2020 16:46:22: 8000000 INFO @ Wed, 22 Jul 2020 16:46:22: 13000000 INFO @ Wed, 22 Jul 2020 16:46:27: 18000000 INFO @ Wed, 22 Jul 2020 16:46:29: 14000000 INFO @ Wed, 22 Jul 2020 16:46:29: 9000000 INFO @ Wed, 22 Jul 2020 16:46:35: 19000000 INFO @ Wed, 22 Jul 2020 16:46:35: 15000000 INFO @ Wed, 22 Jul 2020 16:46:37: 10000000 INFO @ Wed, 22 Jul 2020 16:46:42: 20000000 INFO @ Wed, 22 Jul 2020 16:46:42: 16000000 INFO @ Wed, 22 Jul 2020 16:46:44: 11000000 INFO @ Wed, 22 Jul 2020 16:46:49: 21000000 INFO @ Wed, 22 Jul 2020 16:46:49: 17000000 INFO @ Wed, 22 Jul 2020 16:46:51: 12000000 INFO @ Wed, 22 Jul 2020 16:46:55: 18000000 INFO @ Wed, 22 Jul 2020 16:46:56: 22000000 INFO @ Wed, 22 Jul 2020 16:46:58: 13000000 INFO @ Wed, 22 Jul 2020 16:47:02: 19000000 INFO @ Wed, 22 Jul 2020 16:47:03: 23000000 INFO @ Wed, 22 Jul 2020 16:47:06: 14000000 INFO @ Wed, 22 Jul 2020 16:47:09: 20000000 INFO @ Wed, 22 Jul 2020 16:47:11: 24000000 INFO @ Wed, 22 Jul 2020 16:47:13: 15000000 INFO @ Wed, 22 Jul 2020 16:47:15: 21000000 INFO @ Wed, 22 Jul 2020 16:47:18: 25000000 INFO @ Wed, 22 Jul 2020 16:47:20: 16000000 INFO @ Wed, 22 Jul 2020 16:47:22: 22000000 INFO @ Wed, 22 Jul 2020 16:47:25: 26000000 INFO @ Wed, 22 Jul 2020 16:47:27: 17000000 INFO @ Wed, 22 Jul 2020 16:47:29: 23000000 INFO @ Wed, 22 Jul 2020 16:47:32: 27000000 INFO @ Wed, 22 Jul 2020 16:47:34: 18000000 INFO @ Wed, 22 Jul 2020 16:47:35: 24000000 INFO @ Wed, 22 Jul 2020 16:47:39: 28000000 INFO @ Wed, 22 Jul 2020 16:47:42: 19000000 INFO @ Wed, 22 Jul 2020 16:47:42: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:47:46: 29000000 INFO @ Wed, 22 Jul 2020 16:47:48: 26000000 INFO @ Wed, 22 Jul 2020 16:47:49: 20000000 INFO @ Wed, 22 Jul 2020 16:47:54: 30000000 INFO @ Wed, 22 Jul 2020 16:47:55: 27000000 INFO @ Wed, 22 Jul 2020 16:47:56: 21000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:48:01: 31000000 INFO @ Wed, 22 Jul 2020 16:48:02: 28000000 INFO @ Wed, 22 Jul 2020 16:48:03: 22000000 INFO @ Wed, 22 Jul 2020 16:48:08: 32000000 INFO @ Wed, 22 Jul 2020 16:48:08: 29000000 INFO @ Wed, 22 Jul 2020 16:48:10: 23000000 INFO @ Wed, 22 Jul 2020 16:48:15: 33000000 INFO @ Wed, 22 Jul 2020 16:48:15: 30000000 INFO @ Wed, 22 Jul 2020 16:48:17: 24000000 INFO @ Wed, 22 Jul 2020 16:48:22: 31000000 INFO @ Wed, 22 Jul 2020 16:48:22: 34000000 INFO @ Wed, 22 Jul 2020 16:48:25: 25000000 INFO @ Wed, 22 Jul 2020 16:48:28: 32000000 INFO @ Wed, 22 Jul 2020 16:48:29: 35000000 INFO @ Wed, 22 Jul 2020 16:48:32: 26000000 INFO @ Wed, 22 Jul 2020 16:48:35: 33000000 INFO @ Wed, 22 Jul 2020 16:48:36: 36000000 INFO @ Wed, 22 Jul 2020 16:48:39: 27000000 INFO @ Wed, 22 Jul 2020 16:48:41: 34000000 INFO @ Wed, 22 Jul 2020 16:48:43: 37000000 INFO @ Wed, 22 Jul 2020 16:48:46: 28000000 INFO @ Wed, 22 Jul 2020 16:48:48: 35000000 INFO @ Wed, 22 Jul 2020 16:48:50: 38000000 INFO @ Wed, 22 Jul 2020 16:48:53: 29000000 INFO @ Wed, 22 Jul 2020 16:48:55: 36000000 INFO @ Wed, 22 Jul 2020 16:48:57: 39000000 INFO @ Wed, 22 Jul 2020 16:49:00: 30000000 INFO @ Wed, 22 Jul 2020 16:49:01: 37000000 INFO @ Wed, 22 Jul 2020 16:49:04: 40000000 INFO @ Wed, 22 Jul 2020 16:49:07: 31000000 INFO @ Wed, 22 Jul 2020 16:49:08: 38000000 INFO @ Wed, 22 Jul 2020 16:49:11: 41000000 INFO @ Wed, 22 Jul 2020 16:49:14: 32000000 INFO @ Wed, 22 Jul 2020 16:49:15: 39000000 INFO @ Wed, 22 Jul 2020 16:49:18: 42000000 INFO @ Wed, 22 Jul 2020 16:49:21: 40000000 INFO @ Wed, 22 Jul 2020 16:49:22: 33000000 INFO @ Wed, 22 Jul 2020 16:49:25: 43000000 INFO @ Wed, 22 Jul 2020 16:49:28: 41000000 INFO @ Wed, 22 Jul 2020 16:49:29: 34000000 INFO @ Wed, 22 Jul 2020 16:49:32: 44000000 INFO @ Wed, 22 Jul 2020 16:49:34: 42000000 INFO @ Wed, 22 Jul 2020 16:49:36: 35000000 INFO @ Wed, 22 Jul 2020 16:49:39: 45000000 INFO @ Wed, 22 Jul 2020 16:49:41: 43000000 INFO @ Wed, 22 Jul 2020 16:49:43: 36000000 INFO @ Wed, 22 Jul 2020 16:49:46: 46000000 INFO @ Wed, 22 Jul 2020 16:49:48: 44000000 INFO @ Wed, 22 Jul 2020 16:49:50: 37000000 INFO @ Wed, 22 Jul 2020 16:49:53: 47000000 INFO @ Wed, 22 Jul 2020 16:49:55: 45000000 INFO @ Wed, 22 Jul 2020 16:49:57: 38000000 INFO @ Wed, 22 Jul 2020 16:50:01: 48000000 INFO @ Wed, 22 Jul 2020 16:50:02: 46000000 INFO @ Wed, 22 Jul 2020 16:50:04: 39000000 INFO @ Wed, 22 Jul 2020 16:50:08: 49000000 INFO @ Wed, 22 Jul 2020 16:50:09: 47000000 INFO @ Wed, 22 Jul 2020 16:50:12: 40000000 INFO @ Wed, 22 Jul 2020 16:50:15: 50000000 INFO @ Wed, 22 Jul 2020 16:50:16: 48000000 INFO @ Wed, 22 Jul 2020 16:50:19: 41000000 INFO @ Wed, 22 Jul 2020 16:50:22: 51000000 INFO @ Wed, 22 Jul 2020 16:50:22: 49000000 INFO @ Wed, 22 Jul 2020 16:50:26: 42000000 INFO @ Wed, 22 Jul 2020 16:50:29: 50000000 INFO @ Wed, 22 Jul 2020 16:50:29: 52000000 INFO @ Wed, 22 Jul 2020 16:50:33: 43000000 INFO @ Wed, 22 Jul 2020 16:50:35: 51000000 INFO @ Wed, 22 Jul 2020 16:50:36: 53000000 INFO @ Wed, 22 Jul 2020 16:50:40: 44000000 INFO @ Wed, 22 Jul 2020 16:50:42: 52000000 INFO @ Wed, 22 Jul 2020 16:50:43: 54000000 INFO @ Wed, 22 Jul 2020 16:50:47: 45000000 INFO @ Wed, 22 Jul 2020 16:50:49: 53000000 INFO @ Wed, 22 Jul 2020 16:50:50: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:50:50: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:50:50: #1 total tags in treatment: 26884336 INFO @ Wed, 22 Jul 2020 16:50:50: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:50:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:50:50: #1 tags after filtering in treatment: 15190892 INFO @ Wed, 22 Jul 2020 16:50:50: #1 Redundant rate of treatment: 0.43 INFO @ Wed, 22 Jul 2020 16:50:50: #1 finished! INFO @ Wed, 22 Jul 2020 16:50:50: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:50:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:50:51: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:50:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:50:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:50:54: 46000000 INFO @ Wed, 22 Jul 2020 16:50:55: 54000000 INFO @ Wed, 22 Jul 2020 16:51:01: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:51:01: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:51:01: #1 total tags in treatment: 26884336 INFO @ Wed, 22 Jul 2020 16:51:01: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:51:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:51:01: 47000000 INFO @ Wed, 22 Jul 2020 16:51:02: #1 tags after filtering in treatment: 15190892 INFO @ Wed, 22 Jul 2020 16:51:02: #1 Redundant rate of treatment: 0.43 INFO @ Wed, 22 Jul 2020 16:51:02: #1 finished! INFO @ Wed, 22 Jul 2020 16:51:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:51:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:51:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:51:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:51:08: 48000000 INFO @ Wed, 22 Jul 2020 16:51:14: 49000000 INFO @ Wed, 22 Jul 2020 16:51:21: 50000000 INFO @ Wed, 22 Jul 2020 16:51:27: 51000000 INFO @ Wed, 22 Jul 2020 16:51:34: 52000000 INFO @ Wed, 22 Jul 2020 16:51:40: 53000000 INFO @ Wed, 22 Jul 2020 16:51:46: 54000000 INFO @ Wed, 22 Jul 2020 16:51:52: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:51:52: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:51:52: #1 total tags in treatment: 26884336 INFO @ Wed, 22 Jul 2020 16:51:52: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:51:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:51:53: #1 tags after filtering in treatment: 15190892 INFO @ Wed, 22 Jul 2020 16:51:53: #1 Redundant rate of treatment: 0.43 INFO @ Wed, 22 Jul 2020 16:51:53: #1 finished! INFO @ Wed, 22 Jul 2020 16:51:53: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:51:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:51:54: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:51:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:51:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639587/SRX8639587.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling