Job ID = 7117081 SRX = SRX8639495 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:25:52 prefetch.2.10.7: 1) Downloading 'SRR12117059'... 2020-07-22T06:25:52 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:29:08 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:29:08 prefetch.2.10.7: 1) 'SRR12117059' was downloaded successfully 2020-07-22T06:29:08 prefetch.2.10.7: 'SRR12117059' has 0 unresolved dependencies Read 34652599 spots for SRR12117059/SRR12117059.sra Written 34652599 spots for SRR12117059/SRR12117059.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:13 34652599 reads; of these: 34652599 (100.00%) were paired; of these: 5289594 (15.26%) aligned concordantly 0 times 25288813 (72.98%) aligned concordantly exactly 1 time 4074192 (11.76%) aligned concordantly >1 times ---- 5289594 pairs aligned concordantly 0 times; of these: 640871 (12.12%) aligned discordantly 1 time ---- 4648723 pairs aligned 0 times concordantly or discordantly; of these: 9297446 mates make up the pairs; of these: 8501218 (91.44%) aligned 0 times 484727 (5.21%) aligned exactly 1 time 311501 (3.35%) aligned >1 times 87.73% overall alignment rate Time searching: 00:18:14 Overall time: 00:18:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 913690 / 29698584 = 0.0308 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:03:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:03:19: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:03:19: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:03:23: 1000000 INFO @ Wed, 22 Jul 2020 16:03:28: 2000000 INFO @ Wed, 22 Jul 2020 16:03:32: 3000000 INFO @ Wed, 22 Jul 2020 16:03:36: 4000000 INFO @ Wed, 22 Jul 2020 16:03:41: 5000000 INFO @ Wed, 22 Jul 2020 16:03:45: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:03:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:03:47: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:03:47: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:03:50: 7000000 INFO @ Wed, 22 Jul 2020 16:03:52: 1000000 INFO @ Wed, 22 Jul 2020 16:03:54: 8000000 INFO @ Wed, 22 Jul 2020 16:03:57: 2000000 INFO @ Wed, 22 Jul 2020 16:03:59: 9000000 INFO @ Wed, 22 Jul 2020 16:04:01: 3000000 INFO @ Wed, 22 Jul 2020 16:04:04: 10000000 INFO @ Wed, 22 Jul 2020 16:04:06: 4000000 INFO @ Wed, 22 Jul 2020 16:04:08: 11000000 INFO @ Wed, 22 Jul 2020 16:04:10: 5000000 INFO @ Wed, 22 Jul 2020 16:04:13: 12000000 INFO @ Wed, 22 Jul 2020 16:04:15: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:04:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:04:17: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:04:17: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:04:17: 13000000 INFO @ Wed, 22 Jul 2020 16:04:20: 7000000 INFO @ Wed, 22 Jul 2020 16:04:22: 1000000 INFO @ Wed, 22 Jul 2020 16:04:22: 14000000 INFO @ Wed, 22 Jul 2020 16:04:24: 8000000 INFO @ Wed, 22 Jul 2020 16:04:27: 15000000 INFO @ Wed, 22 Jul 2020 16:04:27: 2000000 INFO @ Wed, 22 Jul 2020 16:04:29: 9000000 INFO @ Wed, 22 Jul 2020 16:04:31: 16000000 INFO @ Wed, 22 Jul 2020 16:04:31: 3000000 INFO @ Wed, 22 Jul 2020 16:04:34: 10000000 INFO @ Wed, 22 Jul 2020 16:04:36: 17000000 INFO @ Wed, 22 Jul 2020 16:04:36: 4000000 INFO @ Wed, 22 Jul 2020 16:04:38: 11000000 INFO @ Wed, 22 Jul 2020 16:04:41: 18000000 INFO @ Wed, 22 Jul 2020 16:04:41: 5000000 INFO @ Wed, 22 Jul 2020 16:04:43: 12000000 INFO @ Wed, 22 Jul 2020 16:04:45: 19000000 INFO @ Wed, 22 Jul 2020 16:04:45: 6000000 INFO @ Wed, 22 Jul 2020 16:04:48: 13000000 INFO @ Wed, 22 Jul 2020 16:04:50: 20000000 INFO @ Wed, 22 Jul 2020 16:04:50: 7000000 INFO @ Wed, 22 Jul 2020 16:04:52: 14000000 INFO @ Wed, 22 Jul 2020 16:04:55: 21000000 INFO @ Wed, 22 Jul 2020 16:04:55: 8000000 INFO @ Wed, 22 Jul 2020 16:04:57: 15000000 INFO @ Wed, 22 Jul 2020 16:04:59: 22000000 INFO @ Wed, 22 Jul 2020 16:04:59: 9000000 INFO @ Wed, 22 Jul 2020 16:05:02: 16000000 INFO @ Wed, 22 Jul 2020 16:05:04: 23000000 INFO @ Wed, 22 Jul 2020 16:05:04: 10000000 INFO @ Wed, 22 Jul 2020 16:05:06: 17000000 INFO @ Wed, 22 Jul 2020 16:05:09: 24000000 INFO @ Wed, 22 Jul 2020 16:05:09: 11000000 INFO @ Wed, 22 Jul 2020 16:05:11: 18000000 INFO @ Wed, 22 Jul 2020 16:05:13: 25000000 INFO @ Wed, 22 Jul 2020 16:05:14: 12000000 INFO @ Wed, 22 Jul 2020 16:05:16: 19000000 INFO @ Wed, 22 Jul 2020 16:05:18: 26000000 INFO @ Wed, 22 Jul 2020 16:05:18: 13000000 INFO @ Wed, 22 Jul 2020 16:05:21: 20000000 INFO @ Wed, 22 Jul 2020 16:05:23: 27000000 INFO @ Wed, 22 Jul 2020 16:05:23: 14000000 INFO @ Wed, 22 Jul 2020 16:05:25: 21000000 INFO @ Wed, 22 Jul 2020 16:05:27: 28000000 INFO @ Wed, 22 Jul 2020 16:05:27: 15000000 INFO @ Wed, 22 Jul 2020 16:05:30: 22000000 INFO @ Wed, 22 Jul 2020 16:05:32: 29000000 INFO @ Wed, 22 Jul 2020 16:05:32: 16000000 INFO @ Wed, 22 Jul 2020 16:05:35: 23000000 INFO @ Wed, 22 Jul 2020 16:05:37: 17000000 INFO @ Wed, 22 Jul 2020 16:05:37: 30000000 INFO @ Wed, 22 Jul 2020 16:05:39: 24000000 INFO @ Wed, 22 Jul 2020 16:05:41: 18000000 INFO @ Wed, 22 Jul 2020 16:05:41: 31000000 INFO @ Wed, 22 Jul 2020 16:05:44: 25000000 INFO @ Wed, 22 Jul 2020 16:05:46: 19000000 INFO @ Wed, 22 Jul 2020 16:05:46: 32000000 INFO @ Wed, 22 Jul 2020 16:05:49: 26000000 INFO @ Wed, 22 Jul 2020 16:05:51: 20000000 INFO @ Wed, 22 Jul 2020 16:05:51: 33000000 INFO @ Wed, 22 Jul 2020 16:05:53: 27000000 INFO @ Wed, 22 Jul 2020 16:05:55: 21000000 INFO @ Wed, 22 Jul 2020 16:05:56: 34000000 INFO @ Wed, 22 Jul 2020 16:05:58: 28000000 INFO @ Wed, 22 Jul 2020 16:06:00: 22000000 INFO @ Wed, 22 Jul 2020 16:06:00: 35000000 INFO @ Wed, 22 Jul 2020 16:06:03: 29000000 INFO @ Wed, 22 Jul 2020 16:06:05: 23000000 INFO @ Wed, 22 Jul 2020 16:06:05: 36000000 INFO @ Wed, 22 Jul 2020 16:06:07: 30000000 INFO @ Wed, 22 Jul 2020 16:06:09: 24000000 INFO @ Wed, 22 Jul 2020 16:06:10: 37000000 INFO @ Wed, 22 Jul 2020 16:06:12: 31000000 INFO @ Wed, 22 Jul 2020 16:06:14: 25000000 INFO @ Wed, 22 Jul 2020 16:06:14: 38000000 INFO @ Wed, 22 Jul 2020 16:06:17: 32000000 INFO @ Wed, 22 Jul 2020 16:06:19: 39000000 INFO @ Wed, 22 Jul 2020 16:06:19: 26000000 INFO @ Wed, 22 Jul 2020 16:06:21: 33000000 INFO @ Wed, 22 Jul 2020 16:06:23: 40000000 INFO @ Wed, 22 Jul 2020 16:06:23: 27000000 INFO @ Wed, 22 Jul 2020 16:06:26: 34000000 INFO @ Wed, 22 Jul 2020 16:06:28: 41000000 INFO @ Wed, 22 Jul 2020 16:06:28: 28000000 INFO @ Wed, 22 Jul 2020 16:06:31: 35000000 INFO @ Wed, 22 Jul 2020 16:06:32: 42000000 INFO @ Wed, 22 Jul 2020 16:06:33: 29000000 INFO @ Wed, 22 Jul 2020 16:06:35: 36000000 INFO @ Wed, 22 Jul 2020 16:06:37: 43000000 INFO @ Wed, 22 Jul 2020 16:06:37: 30000000 INFO @ Wed, 22 Jul 2020 16:06:40: 37000000 INFO @ Wed, 22 Jul 2020 16:06:41: 44000000 INFO @ Wed, 22 Jul 2020 16:06:42: 31000000 INFO @ Wed, 22 Jul 2020 16:06:44: 38000000 INFO @ Wed, 22 Jul 2020 16:06:46: 45000000 INFO @ Wed, 22 Jul 2020 16:06:47: 32000000 INFO @ Wed, 22 Jul 2020 16:06:49: 39000000 INFO @ Wed, 22 Jul 2020 16:06:51: 46000000 INFO @ Wed, 22 Jul 2020 16:06:51: 33000000 INFO @ Wed, 22 Jul 2020 16:06:53: 40000000 INFO @ Wed, 22 Jul 2020 16:06:55: 47000000 INFO @ Wed, 22 Jul 2020 16:06:56: 34000000 INFO @ Wed, 22 Jul 2020 16:06:58: 41000000 INFO @ Wed, 22 Jul 2020 16:07:00: 48000000 INFO @ Wed, 22 Jul 2020 16:07:00: 35000000 INFO @ Wed, 22 Jul 2020 16:07:02: 42000000 INFO @ Wed, 22 Jul 2020 16:07:04: 49000000 INFO @ Wed, 22 Jul 2020 16:07:05: 36000000 INFO @ Wed, 22 Jul 2020 16:07:07: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:07:09: 50000000 INFO @ Wed, 22 Jul 2020 16:07:10: 37000000 INFO @ Wed, 22 Jul 2020 16:07:12: 44000000 INFO @ Wed, 22 Jul 2020 16:07:14: 51000000 INFO @ Wed, 22 Jul 2020 16:07:14: 38000000 INFO @ Wed, 22 Jul 2020 16:07:16: 45000000 INFO @ Wed, 22 Jul 2020 16:07:18: 52000000 INFO @ Wed, 22 Jul 2020 16:07:19: 39000000 INFO @ Wed, 22 Jul 2020 16:07:21: 46000000 INFO @ Wed, 22 Jul 2020 16:07:23: 53000000 INFO @ Wed, 22 Jul 2020 16:07:23: 40000000 INFO @ Wed, 22 Jul 2020 16:07:26: 47000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:07:27: 54000000 INFO @ Wed, 22 Jul 2020 16:07:28: 41000000 INFO @ Wed, 22 Jul 2020 16:07:30: 48000000 INFO @ Wed, 22 Jul 2020 16:07:32: 55000000 INFO @ Wed, 22 Jul 2020 16:07:33: 42000000 INFO @ Wed, 22 Jul 2020 16:07:35: 49000000 INFO @ Wed, 22 Jul 2020 16:07:37: 56000000 INFO @ Wed, 22 Jul 2020 16:07:37: 43000000 INFO @ Wed, 22 Jul 2020 16:07:39: 50000000 INFO @ Wed, 22 Jul 2020 16:07:41: 57000000 INFO @ Wed, 22 Jul 2020 16:07:42: 44000000 INFO @ Wed, 22 Jul 2020 16:07:44: 51000000 INFO @ Wed, 22 Jul 2020 16:07:46: 58000000 INFO @ Wed, 22 Jul 2020 16:07:46: 45000000 INFO @ Wed, 22 Jul 2020 16:07:48: 52000000 INFO @ Wed, 22 Jul 2020 16:07:51: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:07:51: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:07:51: #1 total tags in treatment: 28452482 INFO @ Wed, 22 Jul 2020 16:07:51: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:07:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:07:51: 46000000 INFO @ Wed, 22 Jul 2020 16:07:51: #1 tags after filtering in treatment: 15589508 INFO @ Wed, 22 Jul 2020 16:07:51: #1 Redundant rate of treatment: 0.45 INFO @ Wed, 22 Jul 2020 16:07:51: #1 finished! INFO @ Wed, 22 Jul 2020 16:07:51: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:07:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:07:52: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:07:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:07:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:07:53: 53000000 INFO @ Wed, 22 Jul 2020 16:07:56: 47000000 INFO @ Wed, 22 Jul 2020 16:07:57: 54000000 INFO @ Wed, 22 Jul 2020 16:08:00: 48000000 INFO @ Wed, 22 Jul 2020 16:08:02: 55000000 INFO @ Wed, 22 Jul 2020 16:08:04: 49000000 INFO @ Wed, 22 Jul 2020 16:08:06: 56000000 INFO @ Wed, 22 Jul 2020 16:08:09: 50000000 INFO @ Wed, 22 Jul 2020 16:08:11: 57000000 INFO @ Wed, 22 Jul 2020 16:08:13: 51000000 INFO @ Wed, 22 Jul 2020 16:08:15: 58000000 INFO @ Wed, 22 Jul 2020 16:08:18: 52000000 INFO @ Wed, 22 Jul 2020 16:08:20: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:08:20: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:08:20: #1 total tags in treatment: 28452482 INFO @ Wed, 22 Jul 2020 16:08:20: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:08:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:08:21: #1 tags after filtering in treatment: 15589508 INFO @ Wed, 22 Jul 2020 16:08:21: #1 Redundant rate of treatment: 0.45 INFO @ Wed, 22 Jul 2020 16:08:21: #1 finished! INFO @ Wed, 22 Jul 2020 16:08:21: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:08:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:08:22: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:08:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:08:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:08:22: 53000000 INFO @ Wed, 22 Jul 2020 16:08:27: 54000000 INFO @ Wed, 22 Jul 2020 16:08:31: 55000000 INFO @ Wed, 22 Jul 2020 16:08:35: 56000000 INFO @ Wed, 22 Jul 2020 16:08:40: 57000000 INFO @ Wed, 22 Jul 2020 16:08:44: 58000000 INFO @ Wed, 22 Jul 2020 16:08:49: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:08:49: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:08:49: #1 total tags in treatment: 28452482 INFO @ Wed, 22 Jul 2020 16:08:49: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:08:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:08:49: #1 tags after filtering in treatment: 15589508 INFO @ Wed, 22 Jul 2020 16:08:49: #1 Redundant rate of treatment: 0.45 INFO @ Wed, 22 Jul 2020 16:08:49: #1 finished! INFO @ Wed, 22 Jul 2020 16:08:49: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:08:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:08:50: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:08:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:08:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639495/SRX8639495.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling