Job ID = 7117004 SRX = SRX8639494 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:24:12 prefetch.2.10.7: 1) Downloading 'SRR12117058'... 2020-07-22T06:24:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:28:08 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:28:08 prefetch.2.10.7: 1) 'SRR12117058' was downloaded successfully 2020-07-22T06:28:08 prefetch.2.10.7: 'SRR12117058' has 0 unresolved dependencies Read 36277650 spots for SRR12117058/SRR12117058.sra Written 36277650 spots for SRR12117058/SRR12117058.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:26 36277650 reads; of these: 36277650 (100.00%) were paired; of these: 5756266 (15.87%) aligned concordantly 0 times 26365898 (72.68%) aligned concordantly exactly 1 time 4155486 (11.45%) aligned concordantly >1 times ---- 5756266 pairs aligned concordantly 0 times; of these: 625987 (10.87%) aligned discordantly 1 time ---- 5130279 pairs aligned 0 times concordantly or discordantly; of these: 10260558 mates make up the pairs; of these: 9481276 (92.41%) aligned 0 times 476868 (4.65%) aligned exactly 1 time 302414 (2.95%) aligned >1 times 86.93% overall alignment rate Time searching: 00:17:26 Overall time: 00:17:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 989552 / 30865279 = 0.0321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:00:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:00:56: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:00:56: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:01:02: 1000000 INFO @ Wed, 22 Jul 2020 16:01:07: 2000000 INFO @ Wed, 22 Jul 2020 16:01:12: 3000000 INFO @ Wed, 22 Jul 2020 16:01:17: 4000000 INFO @ Wed, 22 Jul 2020 16:01:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:01:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:01:26: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:01:26: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:01:28: 6000000 INFO @ Wed, 22 Jul 2020 16:01:32: 1000000 INFO @ Wed, 22 Jul 2020 16:01:33: 7000000 INFO @ Wed, 22 Jul 2020 16:01:37: 2000000 INFO @ Wed, 22 Jul 2020 16:01:38: 8000000 INFO @ Wed, 22 Jul 2020 16:01:43: 3000000 INFO @ Wed, 22 Jul 2020 16:01:44: 9000000 INFO @ Wed, 22 Jul 2020 16:01:47: 4000000 INFO @ Wed, 22 Jul 2020 16:01:49: 10000000 INFO @ Wed, 22 Jul 2020 16:01:52: 5000000 INFO @ Wed, 22 Jul 2020 16:01:54: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:01:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:01:56: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:01:56: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:01:58: 6000000 INFO @ Wed, 22 Jul 2020 16:02:00: 12000000 INFO @ Wed, 22 Jul 2020 16:02:01: 1000000 INFO @ Wed, 22 Jul 2020 16:02:03: 7000000 INFO @ Wed, 22 Jul 2020 16:02:05: 13000000 INFO @ Wed, 22 Jul 2020 16:02:06: 2000000 INFO @ Wed, 22 Jul 2020 16:02:09: 8000000 INFO @ Wed, 22 Jul 2020 16:02:11: 14000000 INFO @ Wed, 22 Jul 2020 16:02:11: 3000000 INFO @ Wed, 22 Jul 2020 16:02:14: 9000000 INFO @ Wed, 22 Jul 2020 16:02:16: 4000000 INFO @ Wed, 22 Jul 2020 16:02:16: 15000000 INFO @ Wed, 22 Jul 2020 16:02:19: 10000000 INFO @ Wed, 22 Jul 2020 16:02:20: 5000000 INFO @ Wed, 22 Jul 2020 16:02:21: 16000000 INFO @ Wed, 22 Jul 2020 16:02:25: 11000000 INFO @ Wed, 22 Jul 2020 16:02:25: 6000000 INFO @ Wed, 22 Jul 2020 16:02:27: 17000000 INFO @ Wed, 22 Jul 2020 16:02:30: 7000000 INFO @ Wed, 22 Jul 2020 16:02:30: 12000000 INFO @ Wed, 22 Jul 2020 16:02:32: 18000000 INFO @ Wed, 22 Jul 2020 16:02:35: 8000000 INFO @ Wed, 22 Jul 2020 16:02:35: 13000000 INFO @ Wed, 22 Jul 2020 16:02:37: 19000000 INFO @ Wed, 22 Jul 2020 16:02:39: 9000000 INFO @ Wed, 22 Jul 2020 16:02:41: 14000000 INFO @ Wed, 22 Jul 2020 16:02:43: 20000000 INFO @ Wed, 22 Jul 2020 16:02:44: 10000000 INFO @ Wed, 22 Jul 2020 16:02:46: 15000000 INFO @ Wed, 22 Jul 2020 16:02:48: 21000000 INFO @ Wed, 22 Jul 2020 16:02:49: 11000000 INFO @ Wed, 22 Jul 2020 16:02:51: 16000000 INFO @ Wed, 22 Jul 2020 16:02:54: 22000000 INFO @ Wed, 22 Jul 2020 16:02:54: 12000000 INFO @ Wed, 22 Jul 2020 16:02:56: 17000000 INFO @ Wed, 22 Jul 2020 16:02:58: 13000000 INFO @ Wed, 22 Jul 2020 16:02:59: 23000000 INFO @ Wed, 22 Jul 2020 16:03:02: 18000000 INFO @ Wed, 22 Jul 2020 16:03:03: 14000000 INFO @ Wed, 22 Jul 2020 16:03:04: 24000000 INFO @ Wed, 22 Jul 2020 16:03:07: 19000000 INFO @ Wed, 22 Jul 2020 16:03:08: 15000000 INFO @ Wed, 22 Jul 2020 16:03:10: 25000000 INFO @ Wed, 22 Jul 2020 16:03:12: 20000000 INFO @ Wed, 22 Jul 2020 16:03:13: 16000000 INFO @ Wed, 22 Jul 2020 16:03:15: 26000000 INFO @ Wed, 22 Jul 2020 16:03:17: 17000000 INFO @ Wed, 22 Jul 2020 16:03:17: 21000000 INFO @ Wed, 22 Jul 2020 16:03:21: 27000000 INFO @ Wed, 22 Jul 2020 16:03:22: 18000000 INFO @ Wed, 22 Jul 2020 16:03:23: 22000000 INFO @ Wed, 22 Jul 2020 16:03:26: 28000000 INFO @ Wed, 22 Jul 2020 16:03:27: 19000000 INFO @ Wed, 22 Jul 2020 16:03:28: 23000000 INFO @ Wed, 22 Jul 2020 16:03:31: 20000000 INFO @ Wed, 22 Jul 2020 16:03:31: 29000000 INFO @ Wed, 22 Jul 2020 16:03:33: 24000000 INFO @ Wed, 22 Jul 2020 16:03:36: 21000000 INFO @ Wed, 22 Jul 2020 16:03:37: 30000000 INFO @ Wed, 22 Jul 2020 16:03:39: 25000000 INFO @ Wed, 22 Jul 2020 16:03:41: 22000000 INFO @ Wed, 22 Jul 2020 16:03:42: 31000000 INFO @ Wed, 22 Jul 2020 16:03:44: 26000000 INFO @ Wed, 22 Jul 2020 16:03:45: 23000000 INFO @ Wed, 22 Jul 2020 16:03:47: 32000000 INFO @ Wed, 22 Jul 2020 16:03:49: 27000000 INFO @ Wed, 22 Jul 2020 16:03:50: 24000000 INFO @ Wed, 22 Jul 2020 16:03:53: 33000000 INFO @ Wed, 22 Jul 2020 16:03:54: 28000000 INFO @ Wed, 22 Jul 2020 16:03:55: 25000000 INFO @ Wed, 22 Jul 2020 16:03:58: 34000000 INFO @ Wed, 22 Jul 2020 16:03:59: 29000000 INFO @ Wed, 22 Jul 2020 16:03:59: 26000000 INFO @ Wed, 22 Jul 2020 16:04:03: 35000000 INFO @ Wed, 22 Jul 2020 16:04:04: 30000000 INFO @ Wed, 22 Jul 2020 16:04:04: 27000000 INFO @ Wed, 22 Jul 2020 16:04:09: 36000000 INFO @ Wed, 22 Jul 2020 16:04:09: 28000000 INFO @ Wed, 22 Jul 2020 16:04:09: 31000000 INFO @ Wed, 22 Jul 2020 16:04:13: 29000000 INFO @ Wed, 22 Jul 2020 16:04:14: 37000000 INFO @ Wed, 22 Jul 2020 16:04:14: 32000000 INFO @ Wed, 22 Jul 2020 16:04:18: 30000000 INFO @ Wed, 22 Jul 2020 16:04:19: 38000000 INFO @ Wed, 22 Jul 2020 16:04:19: 33000000 INFO @ Wed, 22 Jul 2020 16:04:23: 31000000 INFO @ Wed, 22 Jul 2020 16:04:24: 39000000 INFO @ Wed, 22 Jul 2020 16:04:25: 34000000 INFO @ Wed, 22 Jul 2020 16:04:27: 32000000 INFO @ Wed, 22 Jul 2020 16:04:30: 40000000 INFO @ Wed, 22 Jul 2020 16:04:30: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:04:32: 33000000 INFO @ Wed, 22 Jul 2020 16:04:35: 41000000 INFO @ Wed, 22 Jul 2020 16:04:35: 36000000 INFO @ Wed, 22 Jul 2020 16:04:37: 34000000 INFO @ Wed, 22 Jul 2020 16:04:40: 42000000 INFO @ Wed, 22 Jul 2020 16:04:40: 37000000 INFO @ Wed, 22 Jul 2020 16:04:41: 35000000 INFO @ Wed, 22 Jul 2020 16:04:45: 43000000 INFO @ Wed, 22 Jul 2020 16:04:46: 38000000 INFO @ Wed, 22 Jul 2020 16:04:46: 36000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:04:50: 37000000 INFO @ Wed, 22 Jul 2020 16:04:51: 44000000 INFO @ Wed, 22 Jul 2020 16:04:51: 39000000 INFO @ Wed, 22 Jul 2020 16:04:55: 38000000 INFO @ Wed, 22 Jul 2020 16:04:56: 45000000 INFO @ Wed, 22 Jul 2020 16:04:56: 40000000 INFO @ Wed, 22 Jul 2020 16:05:00: 39000000 INFO @ Wed, 22 Jul 2020 16:05:01: 46000000 INFO @ Wed, 22 Jul 2020 16:05:01: 41000000 INFO @ Wed, 22 Jul 2020 16:05:04: 40000000 INFO @ Wed, 22 Jul 2020 16:05:06: 47000000 INFO @ Wed, 22 Jul 2020 16:05:07: 42000000 INFO @ Wed, 22 Jul 2020 16:05:09: 41000000 INFO @ Wed, 22 Jul 2020 16:05:12: 48000000 INFO @ Wed, 22 Jul 2020 16:05:12: 43000000 INFO @ Wed, 22 Jul 2020 16:05:13: 42000000 INFO @ Wed, 22 Jul 2020 16:05:17: 49000000 INFO @ Wed, 22 Jul 2020 16:05:17: 44000000 INFO @ Wed, 22 Jul 2020 16:05:18: 43000000 INFO @ Wed, 22 Jul 2020 16:05:22: 50000000 INFO @ Wed, 22 Jul 2020 16:05:22: 45000000 INFO @ Wed, 22 Jul 2020 16:05:22: 44000000 INFO @ Wed, 22 Jul 2020 16:05:27: 45000000 INFO @ Wed, 22 Jul 2020 16:05:27: 51000000 INFO @ Wed, 22 Jul 2020 16:05:28: 46000000 INFO @ Wed, 22 Jul 2020 16:05:32: 46000000 INFO @ Wed, 22 Jul 2020 16:05:33: 52000000 INFO @ Wed, 22 Jul 2020 16:05:33: 47000000 INFO @ Wed, 22 Jul 2020 16:05:36: 47000000 INFO @ Wed, 22 Jul 2020 16:05:38: 53000000 INFO @ Wed, 22 Jul 2020 16:05:38: 48000000 INFO @ Wed, 22 Jul 2020 16:05:41: 48000000 INFO @ Wed, 22 Jul 2020 16:05:43: 54000000 INFO @ Wed, 22 Jul 2020 16:05:43: 49000000 INFO @ Wed, 22 Jul 2020 16:05:45: 49000000 INFO @ Wed, 22 Jul 2020 16:05:48: 50000000 INFO @ Wed, 22 Jul 2020 16:05:48: 55000000 INFO @ Wed, 22 Jul 2020 16:05:50: 50000000 INFO @ Wed, 22 Jul 2020 16:05:54: 51000000 INFO @ Wed, 22 Jul 2020 16:05:54: 56000000 INFO @ Wed, 22 Jul 2020 16:05:55: 51000000 INFO @ Wed, 22 Jul 2020 16:05:59: 52000000 INFO @ Wed, 22 Jul 2020 16:05:59: 57000000 INFO @ Wed, 22 Jul 2020 16:06:00: 52000000 INFO @ Wed, 22 Jul 2020 16:06:04: 53000000 INFO @ Wed, 22 Jul 2020 16:06:04: 58000000 INFO @ Wed, 22 Jul 2020 16:06:05: 53000000 INFO @ Wed, 22 Jul 2020 16:06:09: 54000000 INFO @ Wed, 22 Jul 2020 16:06:10: 59000000 INFO @ Wed, 22 Jul 2020 16:06:10: 54000000 INFO @ Wed, 22 Jul 2020 16:06:15: 55000000 INFO @ Wed, 22 Jul 2020 16:06:15: 60000000 INFO @ Wed, 22 Jul 2020 16:06:15: 55000000 INFO @ Wed, 22 Jul 2020 16:06:20: 56000000 INFO @ Wed, 22 Jul 2020 16:06:21: 61000000 INFO @ Wed, 22 Jul 2020 16:06:21: 56000000 INFO @ Wed, 22 Jul 2020 16:06:21: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:06:21: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:06:21: #1 total tags in treatment: 29535099 INFO @ Wed, 22 Jul 2020 16:06:21: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:06:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:06:22: #1 tags after filtering in treatment: 15942745 INFO @ Wed, 22 Jul 2020 16:06:22: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 16:06:22: #1 finished! INFO @ Wed, 22 Jul 2020 16:06:22: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:06:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:06:23: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:06:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:06:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:06:25: 57000000 INFO @ Wed, 22 Jul 2020 16:06:26: 57000000 INFO @ Wed, 22 Jul 2020 16:06:30: 58000000 INFO @ Wed, 22 Jul 2020 16:06:31: 58000000 INFO @ Wed, 22 Jul 2020 16:06:36: 59000000 INFO @ Wed, 22 Jul 2020 16:06:36: 59000000 INFO @ Wed, 22 Jul 2020 16:06:41: 60000000 INFO @ Wed, 22 Jul 2020 16:06:42: 60000000 INFO @ Wed, 22 Jul 2020 16:06:46: 61000000 INFO @ Wed, 22 Jul 2020 16:06:47: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:06:47: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:06:47: #1 total tags in treatment: 29535099 INFO @ Wed, 22 Jul 2020 16:06:47: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:06:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:06:47: 61000000 INFO @ Wed, 22 Jul 2020 16:06:47: #1 tags after filtering in treatment: 15942745 INFO @ Wed, 22 Jul 2020 16:06:47: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 16:06:47: #1 finished! INFO @ Wed, 22 Jul 2020 16:06:47: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:06:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:06:47: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:06:47: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:06:47: #1 total tags in treatment: 29535099 INFO @ Wed, 22 Jul 2020 16:06:47: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:06:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:06:48: #1 tags after filtering in treatment: 15942745 INFO @ Wed, 22 Jul 2020 16:06:48: #1 Redundant rate of treatment: 0.46 INFO @ Wed, 22 Jul 2020 16:06:48: #1 finished! INFO @ Wed, 22 Jul 2020 16:06:48: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:06:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:06:48: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:06:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:06:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:06:49: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:06:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:06:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639494/SRX8639494.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling