Job ID = 7116990 SRX = SRX8639493 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:22:07 prefetch.2.10.7: 1) Downloading 'SRR12117057'... 2020-07-22T06:22:07 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:28:07 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:28:07 prefetch.2.10.7: 1) 'SRR12117057' was downloaded successfully 2020-07-22T06:28:07 prefetch.2.10.7: 'SRR12117057' has 0 unresolved dependencies Read 39540104 spots for SRR12117057/SRR12117057.sra Written 39540104 spots for SRR12117057/SRR12117057.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:22 39540104 reads; of these: 39540104 (100.00%) were paired; of these: 3705003 (9.37%) aligned concordantly 0 times 30817676 (77.94%) aligned concordantly exactly 1 time 5017425 (12.69%) aligned concordantly >1 times ---- 3705003 pairs aligned concordantly 0 times; of these: 467981 (12.63%) aligned discordantly 1 time ---- 3237022 pairs aligned 0 times concordantly or discordantly; of these: 6474044 mates make up the pairs; of these: 5859031 (90.50%) aligned 0 times 404730 (6.25%) aligned exactly 1 time 210283 (3.25%) aligned >1 times 92.59% overall alignment rate Time searching: 00:18:22 Overall time: 00:18:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1338828 / 36103922 = 0.0371 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:03:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:03:34: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:03:34: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:03:39: 1000000 INFO @ Wed, 22 Jul 2020 16:03:46: 2000000 INFO @ Wed, 22 Jul 2020 16:03:52: 3000000 INFO @ Wed, 22 Jul 2020 16:03:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:04:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:04:03: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:04:03: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:04:04: 5000000 INFO @ Wed, 22 Jul 2020 16:04:10: 1000000 INFO @ Wed, 22 Jul 2020 16:04:11: 6000000 INFO @ Wed, 22 Jul 2020 16:04:16: 2000000 INFO @ Wed, 22 Jul 2020 16:04:18: 7000000 INFO @ Wed, 22 Jul 2020 16:04:22: 3000000 INFO @ Wed, 22 Jul 2020 16:04:25: 8000000 INFO @ Wed, 22 Jul 2020 16:04:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:04:32: 9000000 INFO @ Wed, 22 Jul 2020 16:04:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:04:33: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:04:33: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:04:35: 5000000 INFO @ Wed, 22 Jul 2020 16:04:39: 10000000 INFO @ Wed, 22 Jul 2020 16:04:40: 1000000 INFO @ Wed, 22 Jul 2020 16:04:41: 6000000 INFO @ Wed, 22 Jul 2020 16:04:45: 11000000 INFO @ Wed, 22 Jul 2020 16:04:47: 2000000 INFO @ Wed, 22 Jul 2020 16:04:48: 7000000 INFO @ Wed, 22 Jul 2020 16:04:52: 12000000 INFO @ Wed, 22 Jul 2020 16:04:54: 3000000 INFO @ Wed, 22 Jul 2020 16:04:54: 8000000 INFO @ Wed, 22 Jul 2020 16:04:59: 13000000 INFO @ Wed, 22 Jul 2020 16:05:00: 4000000 INFO @ Wed, 22 Jul 2020 16:05:01: 9000000 INFO @ Wed, 22 Jul 2020 16:05:07: 14000000 INFO @ Wed, 22 Jul 2020 16:05:07: 5000000 INFO @ Wed, 22 Jul 2020 16:05:07: 10000000 INFO @ Wed, 22 Jul 2020 16:05:14: 15000000 INFO @ Wed, 22 Jul 2020 16:05:14: 11000000 INFO @ Wed, 22 Jul 2020 16:05:14: 6000000 INFO @ Wed, 22 Jul 2020 16:05:20: 12000000 INFO @ Wed, 22 Jul 2020 16:05:20: 7000000 INFO @ Wed, 22 Jul 2020 16:05:21: 16000000 INFO @ Wed, 22 Jul 2020 16:05:26: 13000000 INFO @ Wed, 22 Jul 2020 16:05:27: 8000000 INFO @ Wed, 22 Jul 2020 16:05:27: 17000000 INFO @ Wed, 22 Jul 2020 16:05:33: 14000000 INFO @ Wed, 22 Jul 2020 16:05:34: 9000000 INFO @ Wed, 22 Jul 2020 16:05:35: 18000000 INFO @ Wed, 22 Jul 2020 16:05:39: 15000000 INFO @ Wed, 22 Jul 2020 16:05:40: 10000000 INFO @ Wed, 22 Jul 2020 16:05:41: 19000000 INFO @ Wed, 22 Jul 2020 16:05:46: 16000000 INFO @ Wed, 22 Jul 2020 16:05:47: 11000000 INFO @ Wed, 22 Jul 2020 16:05:48: 20000000 INFO @ Wed, 22 Jul 2020 16:05:52: 17000000 INFO @ Wed, 22 Jul 2020 16:05:54: 12000000 INFO @ Wed, 22 Jul 2020 16:05:55: 21000000 INFO @ Wed, 22 Jul 2020 16:05:59: 18000000 INFO @ Wed, 22 Jul 2020 16:06:01: 13000000 INFO @ Wed, 22 Jul 2020 16:06:02: 22000000 INFO @ Wed, 22 Jul 2020 16:06:05: 19000000 INFO @ Wed, 22 Jul 2020 16:06:07: 14000000 INFO @ Wed, 22 Jul 2020 16:06:09: 23000000 INFO @ Wed, 22 Jul 2020 16:06:11: 20000000 INFO @ Wed, 22 Jul 2020 16:06:14: 15000000 INFO @ Wed, 22 Jul 2020 16:06:16: 24000000 INFO @ Wed, 22 Jul 2020 16:06:18: 21000000 INFO @ Wed, 22 Jul 2020 16:06:21: 16000000 INFO @ Wed, 22 Jul 2020 16:06:23: 25000000 INFO @ Wed, 22 Jul 2020 16:06:24: 22000000 INFO @ Wed, 22 Jul 2020 16:06:28: 17000000 INFO @ Wed, 22 Jul 2020 16:06:31: 26000000 INFO @ Wed, 22 Jul 2020 16:06:31: 23000000 INFO @ Wed, 22 Jul 2020 16:06:34: 18000000 INFO @ Wed, 22 Jul 2020 16:06:37: 24000000 INFO @ Wed, 22 Jul 2020 16:06:38: 27000000 INFO @ Wed, 22 Jul 2020 16:06:41: 19000000 INFO @ Wed, 22 Jul 2020 16:06:44: 25000000 INFO @ Wed, 22 Jul 2020 16:06:45: 28000000 INFO @ Wed, 22 Jul 2020 16:06:48: 20000000 INFO @ Wed, 22 Jul 2020 16:06:50: 26000000 INFO @ Wed, 22 Jul 2020 16:06:52: 29000000 INFO @ Wed, 22 Jul 2020 16:06:55: 21000000 INFO @ Wed, 22 Jul 2020 16:06:57: 27000000 INFO @ Wed, 22 Jul 2020 16:06:59: 30000000 INFO @ Wed, 22 Jul 2020 16:07:01: 22000000 INFO @ Wed, 22 Jul 2020 16:07:03: 28000000 INFO @ Wed, 22 Jul 2020 16:07:06: 31000000 INFO @ Wed, 22 Jul 2020 16:07:08: 23000000 INFO @ Wed, 22 Jul 2020 16:07:10: 29000000 INFO @ Wed, 22 Jul 2020 16:07:13: 32000000 INFO @ Wed, 22 Jul 2020 16:07:15: 24000000 INFO @ Wed, 22 Jul 2020 16:07:16: 30000000 INFO @ Wed, 22 Jul 2020 16:07:21: 33000000 INFO @ Wed, 22 Jul 2020 16:07:22: 25000000 INFO @ Wed, 22 Jul 2020 16:07:23: 31000000 INFO @ Wed, 22 Jul 2020 16:07:28: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:07:29: 26000000 INFO @ Wed, 22 Jul 2020 16:07:29: 32000000 INFO @ Wed, 22 Jul 2020 16:07:35: 35000000 INFO @ Wed, 22 Jul 2020 16:07:36: 27000000 INFO @ Wed, 22 Jul 2020 16:07:36: 33000000 INFO @ Wed, 22 Jul 2020 16:07:42: 36000000 INFO @ Wed, 22 Jul 2020 16:07:42: 34000000 INFO @ Wed, 22 Jul 2020 16:07:43: 28000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:07:49: 37000000 INFO @ Wed, 22 Jul 2020 16:07:49: 35000000 INFO @ Wed, 22 Jul 2020 16:07:49: 29000000 INFO @ Wed, 22 Jul 2020 16:07:55: 36000000 INFO @ Wed, 22 Jul 2020 16:07:56: 38000000 INFO @ Wed, 22 Jul 2020 16:07:56: 30000000 INFO @ Wed, 22 Jul 2020 16:08:02: 37000000 INFO @ Wed, 22 Jul 2020 16:08:03: 39000000 INFO @ Wed, 22 Jul 2020 16:08:03: 31000000 INFO @ Wed, 22 Jul 2020 16:08:08: 38000000 INFO @ Wed, 22 Jul 2020 16:08:10: 40000000 INFO @ Wed, 22 Jul 2020 16:08:10: 32000000 INFO @ Wed, 22 Jul 2020 16:08:15: 39000000 INFO @ Wed, 22 Jul 2020 16:08:17: 33000000 INFO @ Wed, 22 Jul 2020 16:08:17: 41000000 INFO @ Wed, 22 Jul 2020 16:08:21: 40000000 INFO @ Wed, 22 Jul 2020 16:08:24: 34000000 INFO @ Wed, 22 Jul 2020 16:08:24: 42000000 INFO @ Wed, 22 Jul 2020 16:08:28: 41000000 INFO @ Wed, 22 Jul 2020 16:08:31: 35000000 INFO @ Wed, 22 Jul 2020 16:08:31: 43000000 INFO @ Wed, 22 Jul 2020 16:08:34: 42000000 INFO @ Wed, 22 Jul 2020 16:08:38: 36000000 INFO @ Wed, 22 Jul 2020 16:08:39: 44000000 INFO @ Wed, 22 Jul 2020 16:08:41: 43000000 INFO @ Wed, 22 Jul 2020 16:08:44: 37000000 INFO @ Wed, 22 Jul 2020 16:08:46: 45000000 INFO @ Wed, 22 Jul 2020 16:08:47: 44000000 INFO @ Wed, 22 Jul 2020 16:08:51: 38000000 INFO @ Wed, 22 Jul 2020 16:08:53: 46000000 INFO @ Wed, 22 Jul 2020 16:08:54: 45000000 INFO @ Wed, 22 Jul 2020 16:08:58: 39000000 INFO @ Wed, 22 Jul 2020 16:09:00: 47000000 INFO @ Wed, 22 Jul 2020 16:09:00: 46000000 INFO @ Wed, 22 Jul 2020 16:09:05: 40000000 INFO @ Wed, 22 Jul 2020 16:09:07: 47000000 INFO @ Wed, 22 Jul 2020 16:09:07: 48000000 INFO @ Wed, 22 Jul 2020 16:09:12: 41000000 INFO @ Wed, 22 Jul 2020 16:09:13: 48000000 INFO @ Wed, 22 Jul 2020 16:09:14: 49000000 INFO @ Wed, 22 Jul 2020 16:09:19: 42000000 INFO @ Wed, 22 Jul 2020 16:09:20: 49000000 INFO @ Wed, 22 Jul 2020 16:09:22: 50000000 INFO @ Wed, 22 Jul 2020 16:09:26: 43000000 INFO @ Wed, 22 Jul 2020 16:09:27: 50000000 INFO @ Wed, 22 Jul 2020 16:09:29: 51000000 INFO @ Wed, 22 Jul 2020 16:09:32: 44000000 INFO @ Wed, 22 Jul 2020 16:09:33: 51000000 INFO @ Wed, 22 Jul 2020 16:09:36: 52000000 INFO @ Wed, 22 Jul 2020 16:09:39: 45000000 INFO @ Wed, 22 Jul 2020 16:09:40: 52000000 INFO @ Wed, 22 Jul 2020 16:09:43: 53000000 INFO @ Wed, 22 Jul 2020 16:09:46: 46000000 INFO @ Wed, 22 Jul 2020 16:09:47: 53000000 INFO @ Wed, 22 Jul 2020 16:09:50: 54000000 INFO @ Wed, 22 Jul 2020 16:09:53: 47000000 INFO @ Wed, 22 Jul 2020 16:09:53: 54000000 INFO @ Wed, 22 Jul 2020 16:09:57: 55000000 INFO @ Wed, 22 Jul 2020 16:10:00: 48000000 INFO @ Wed, 22 Jul 2020 16:10:00: 55000000 INFO @ Wed, 22 Jul 2020 16:10:04: 56000000 INFO @ Wed, 22 Jul 2020 16:10:06: 56000000 INFO @ Wed, 22 Jul 2020 16:10:07: 49000000 INFO @ Wed, 22 Jul 2020 16:10:12: 57000000 INFO @ Wed, 22 Jul 2020 16:10:13: 57000000 INFO @ Wed, 22 Jul 2020 16:10:13: 50000000 INFO @ Wed, 22 Jul 2020 16:10:19: 58000000 INFO @ Wed, 22 Jul 2020 16:10:20: 58000000 INFO @ Wed, 22 Jul 2020 16:10:20: 51000000 INFO @ Wed, 22 Jul 2020 16:10:26: 59000000 INFO @ Wed, 22 Jul 2020 16:10:27: 59000000 INFO @ Wed, 22 Jul 2020 16:10:27: 52000000 INFO @ Wed, 22 Jul 2020 16:10:33: 60000000 INFO @ Wed, 22 Jul 2020 16:10:34: 53000000 INFO @ Wed, 22 Jul 2020 16:10:34: 60000000 INFO @ Wed, 22 Jul 2020 16:10:41: 61000000 INFO @ Wed, 22 Jul 2020 16:10:41: 54000000 INFO @ Wed, 22 Jul 2020 16:10:42: 61000000 INFO @ Wed, 22 Jul 2020 16:10:48: 55000000 INFO @ Wed, 22 Jul 2020 16:10:48: 62000000 INFO @ Wed, 22 Jul 2020 16:10:49: 62000000 INFO @ Wed, 22 Jul 2020 16:10:55: 56000000 INFO @ Wed, 22 Jul 2020 16:10:55: 63000000 INFO @ Wed, 22 Jul 2020 16:10:56: 63000000 INFO @ Wed, 22 Jul 2020 16:11:01: 57000000 INFO @ Wed, 22 Jul 2020 16:11:02: 64000000 INFO @ Wed, 22 Jul 2020 16:11:03: 64000000 INFO @ Wed, 22 Jul 2020 16:11:08: 58000000 INFO @ Wed, 22 Jul 2020 16:11:10: 65000000 INFO @ Wed, 22 Jul 2020 16:11:11: 65000000 INFO @ Wed, 22 Jul 2020 16:11:15: 59000000 INFO @ Wed, 22 Jul 2020 16:11:17: 66000000 INFO @ Wed, 22 Jul 2020 16:11:18: 66000000 INFO @ Wed, 22 Jul 2020 16:11:22: 60000000 INFO @ Wed, 22 Jul 2020 16:11:24: 67000000 INFO @ Wed, 22 Jul 2020 16:11:25: 67000000 INFO @ Wed, 22 Jul 2020 16:11:29: 61000000 INFO @ Wed, 22 Jul 2020 16:11:32: 68000000 INFO @ Wed, 22 Jul 2020 16:11:33: 68000000 INFO @ Wed, 22 Jul 2020 16:11:36: 62000000 INFO @ Wed, 22 Jul 2020 16:11:39: 69000000 INFO @ Wed, 22 Jul 2020 16:11:40: 69000000 INFO @ Wed, 22 Jul 2020 16:11:42: 63000000 INFO @ Wed, 22 Jul 2020 16:11:46: 70000000 INFO @ Wed, 22 Jul 2020 16:11:47: 70000000 INFO @ Wed, 22 Jul 2020 16:11:49: 64000000 INFO @ Wed, 22 Jul 2020 16:11:50: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:11:50: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:11:50: #1 total tags in treatment: 34496694 INFO @ Wed, 22 Jul 2020 16:11:50: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:11:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:11:51: #1 tags after filtering in treatment: 17513501 INFO @ Wed, 22 Jul 2020 16:11:51: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 16:11:51: #1 finished! INFO @ Wed, 22 Jul 2020 16:11:51: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:11:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:11:51: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:11:51: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:11:51: #1 total tags in treatment: 34496694 INFO @ Wed, 22 Jul 2020 16:11:51: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:11:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:11:52: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:11:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:11:52: Process for pairing-model is terminated! INFO @ Wed, 22 Jul 2020 16:11:52: #1 tags after filtering in treatment: 17513501 INFO @ Wed, 22 Jul 2020 16:11:52: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 16:11:52: #1 finished! INFO @ Wed, 22 Jul 2020 16:11:52: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:11:52: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:11:53: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:11:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:11:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:11:56: 65000000 INFO @ Wed, 22 Jul 2020 16:12:02: 66000000 INFO @ Wed, 22 Jul 2020 16:12:08: 67000000 INFO @ Wed, 22 Jul 2020 16:12:14: 68000000 INFO @ Wed, 22 Jul 2020 16:12:20: 69000000 INFO @ Wed, 22 Jul 2020 16:12:26: 70000000 INFO @ Wed, 22 Jul 2020 16:12:29: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:12:29: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:12:29: #1 total tags in treatment: 34496694 INFO @ Wed, 22 Jul 2020 16:12:29: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:12:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:12:30: #1 tags after filtering in treatment: 17513501 INFO @ Wed, 22 Jul 2020 16:12:30: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 16:12:30: #1 finished! INFO @ Wed, 22 Jul 2020 16:12:30: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:12:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:12:31: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:12:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:12:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639493/SRX8639493.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling