Job ID = 7116909 SRX = SRX8639491 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:21:07 prefetch.2.10.7: 1) Downloading 'SRR12117055'... 2020-07-22T06:21:07 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:26:34 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:26:34 prefetch.2.10.7: 1) 'SRR12117055' was downloaded successfully 2020-07-22T06:26:34 prefetch.2.10.7: 'SRR12117055' has 0 unresolved dependencies Read 42774056 spots for SRR12117055/SRR12117055.sra Written 42774056 spots for SRR12117055/SRR12117055.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:29 42774056 reads; of these: 42774056 (100.00%) were paired; of these: 4087740 (9.56%) aligned concordantly 0 times 32400649 (75.75%) aligned concordantly exactly 1 time 6285667 (14.70%) aligned concordantly >1 times ---- 4087740 pairs aligned concordantly 0 times; of these: 505734 (12.37%) aligned discordantly 1 time ---- 3582006 pairs aligned 0 times concordantly or discordantly; of these: 7164012 mates make up the pairs; of these: 6308775 (88.06%) aligned 0 times 530931 (7.41%) aligned exactly 1 time 324306 (4.53%) aligned >1 times 92.63% overall alignment rate Time searching: 00:25:29 Overall time: 00:25:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2049153 / 38882357 = 0.0527 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:12:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:12:17: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:12:17: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:12:22: 1000000 INFO @ Wed, 22 Jul 2020 16:12:27: 2000000 INFO @ Wed, 22 Jul 2020 16:12:31: 3000000 INFO @ Wed, 22 Jul 2020 16:12:36: 4000000 INFO @ Wed, 22 Jul 2020 16:12:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:12:46: 6000000 INFO @ Wed, 22 Jul 2020 16:12:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:12:47: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:12:47: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:12:51: 7000000 INFO @ Wed, 22 Jul 2020 16:12:52: 1000000 INFO @ Wed, 22 Jul 2020 16:12:56: 8000000 INFO @ Wed, 22 Jul 2020 16:12:57: 2000000 INFO @ Wed, 22 Jul 2020 16:13:02: 9000000 INFO @ Wed, 22 Jul 2020 16:13:03: 3000000 INFO @ Wed, 22 Jul 2020 16:13:07: 10000000 INFO @ Wed, 22 Jul 2020 16:13:08: 4000000 INFO @ Wed, 22 Jul 2020 16:13:12: 11000000 INFO @ Wed, 22 Jul 2020 16:13:13: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 16:13:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 16:13:17: #1 read tag files... INFO @ Wed, 22 Jul 2020 16:13:17: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 16:13:17: 12000000 INFO @ Wed, 22 Jul 2020 16:13:18: 6000000 INFO @ Wed, 22 Jul 2020 16:13:22: 1000000 INFO @ Wed, 22 Jul 2020 16:13:23: 13000000 INFO @ Wed, 22 Jul 2020 16:13:24: 7000000 INFO @ Wed, 22 Jul 2020 16:13:27: 2000000 INFO @ Wed, 22 Jul 2020 16:13:28: 14000000 INFO @ Wed, 22 Jul 2020 16:13:29: 8000000 INFO @ Wed, 22 Jul 2020 16:13:33: 3000000 INFO @ Wed, 22 Jul 2020 16:13:33: 15000000 INFO @ Wed, 22 Jul 2020 16:13:34: 9000000 INFO @ Wed, 22 Jul 2020 16:13:38: 4000000 INFO @ Wed, 22 Jul 2020 16:13:38: 16000000 INFO @ Wed, 22 Jul 2020 16:13:40: 10000000 INFO @ Wed, 22 Jul 2020 16:13:43: 5000000 INFO @ Wed, 22 Jul 2020 16:13:44: 17000000 INFO @ Wed, 22 Jul 2020 16:13:45: 11000000 INFO @ Wed, 22 Jul 2020 16:13:49: 6000000 INFO @ Wed, 22 Jul 2020 16:13:49: 18000000 INFO @ Wed, 22 Jul 2020 16:13:50: 12000000 INFO @ Wed, 22 Jul 2020 16:13:54: 7000000 INFO @ Wed, 22 Jul 2020 16:13:54: 19000000 INFO @ Wed, 22 Jul 2020 16:13:56: 13000000 INFO @ Wed, 22 Jul 2020 16:14:00: 8000000 INFO @ Wed, 22 Jul 2020 16:14:00: 20000000 INFO @ Wed, 22 Jul 2020 16:14:01: 14000000 INFO @ Wed, 22 Jul 2020 16:14:05: 9000000 INFO @ Wed, 22 Jul 2020 16:14:06: 21000000 INFO @ Wed, 22 Jul 2020 16:14:07: 15000000 INFO @ Wed, 22 Jul 2020 16:14:11: 10000000 INFO @ Wed, 22 Jul 2020 16:14:12: 22000000 INFO @ Wed, 22 Jul 2020 16:14:12: 16000000 INFO @ Wed, 22 Jul 2020 16:14:16: 11000000 INFO @ Wed, 22 Jul 2020 16:14:17: 23000000 INFO @ Wed, 22 Jul 2020 16:14:18: 17000000 INFO @ Wed, 22 Jul 2020 16:14:22: 12000000 INFO @ Wed, 22 Jul 2020 16:14:22: 24000000 INFO @ Wed, 22 Jul 2020 16:14:23: 18000000 INFO @ Wed, 22 Jul 2020 16:14:27: 13000000 INFO @ Wed, 22 Jul 2020 16:14:28: 25000000 INFO @ Wed, 22 Jul 2020 16:14:29: 19000000 INFO @ Wed, 22 Jul 2020 16:14:32: 14000000 INFO @ Wed, 22 Jul 2020 16:14:33: 26000000 INFO @ Wed, 22 Jul 2020 16:14:34: 20000000 INFO @ Wed, 22 Jul 2020 16:14:38: 15000000 INFO @ Wed, 22 Jul 2020 16:14:39: 27000000 INFO @ Wed, 22 Jul 2020 16:14:40: 21000000 INFO @ Wed, 22 Jul 2020 16:14:43: 16000000 INFO @ Wed, 22 Jul 2020 16:14:44: 28000000 INFO @ Wed, 22 Jul 2020 16:14:46: 22000000 INFO @ Wed, 22 Jul 2020 16:14:49: 17000000 INFO @ Wed, 22 Jul 2020 16:14:49: 29000000 INFO @ Wed, 22 Jul 2020 16:14:51: 23000000 INFO @ Wed, 22 Jul 2020 16:14:54: 18000000 INFO @ Wed, 22 Jul 2020 16:14:55: 30000000 INFO @ Wed, 22 Jul 2020 16:14:57: 24000000 INFO @ Wed, 22 Jul 2020 16:15:00: 19000000 INFO @ Wed, 22 Jul 2020 16:15:00: 31000000 INFO @ Wed, 22 Jul 2020 16:15:02: 25000000 INFO @ Wed, 22 Jul 2020 16:15:05: 20000000 INFO @ Wed, 22 Jul 2020 16:15:06: 32000000 INFO @ Wed, 22 Jul 2020 16:15:08: 26000000 INFO @ Wed, 22 Jul 2020 16:15:11: 33000000 INFO @ Wed, 22 Jul 2020 16:15:11: 21000000 INFO @ Wed, 22 Jul 2020 16:15:13: 27000000 INFO @ Wed, 22 Jul 2020 16:15:16: 34000000 INFO @ Wed, 22 Jul 2020 16:15:17: 22000000 INFO @ Wed, 22 Jul 2020 16:15:19: 28000000 INFO @ Wed, 22 Jul 2020 16:15:22: 35000000 INFO @ Wed, 22 Jul 2020 16:15:23: 23000000 INFO @ Wed, 22 Jul 2020 16:15:24: 29000000 INFO @ Wed, 22 Jul 2020 16:15:27: 36000000 INFO @ Wed, 22 Jul 2020 16:15:28: 24000000 INFO @ Wed, 22 Jul 2020 16:15:30: 30000000 INFO @ Wed, 22 Jul 2020 16:15:33: 37000000 INFO @ Wed, 22 Jul 2020 16:15:34: 25000000 INFO @ Wed, 22 Jul 2020 16:15:35: 31000000 INFO @ Wed, 22 Jul 2020 16:15:38: 38000000 INFO @ Wed, 22 Jul 2020 16:15:39: 26000000 INFO @ Wed, 22 Jul 2020 16:15:41: 32000000 INFO @ Wed, 22 Jul 2020 16:15:44: 39000000 INFO @ Wed, 22 Jul 2020 16:15:45: 27000000 INFO @ Wed, 22 Jul 2020 16:15:47: 33000000 INFO @ Wed, 22 Jul 2020 16:15:49: 40000000 INFO @ Wed, 22 Jul 2020 16:15:50: 28000000 INFO @ Wed, 22 Jul 2020 16:15:52: 34000000 INFO @ Wed, 22 Jul 2020 16:15:54: 41000000 INFO @ Wed, 22 Jul 2020 16:15:56: 29000000 INFO @ Wed, 22 Jul 2020 16:15:58: 35000000 INFO @ Wed, 22 Jul 2020 16:16:00: 42000000 INFO @ Wed, 22 Jul 2020 16:16:02: 30000000 INFO @ Wed, 22 Jul 2020 16:16:03: 36000000 INFO @ Wed, 22 Jul 2020 16:16:05: 43000000 INFO @ Wed, 22 Jul 2020 16:16:07: 31000000 INFO @ Wed, 22 Jul 2020 16:16:09: 37000000 INFO @ Wed, 22 Jul 2020 16:16:11: 44000000 INFO @ Wed, 22 Jul 2020 16:16:13: 32000000 INFO @ Wed, 22 Jul 2020 16:16:14: 38000000 INFO @ Wed, 22 Jul 2020 16:16:16: 45000000 INFO @ Wed, 22 Jul 2020 16:16:18: 33000000 INFO @ Wed, 22 Jul 2020 16:16:20: 39000000 INFO @ Wed, 22 Jul 2020 16:16:22: 46000000 INFO @ Wed, 22 Jul 2020 16:16:24: 34000000 INFO @ Wed, 22 Jul 2020 16:16:25: 40000000 INFO @ Wed, 22 Jul 2020 16:16:27: 47000000 INFO @ Wed, 22 Jul 2020 16:16:29: 35000000 INFO @ Wed, 22 Jul 2020 16:16:31: 41000000 INFO @ Wed, 22 Jul 2020 16:16:32: 48000000 INFO @ Wed, 22 Jul 2020 16:16:35: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 16:16:36: 42000000 INFO @ Wed, 22 Jul 2020 16:16:38: 49000000 INFO @ Wed, 22 Jul 2020 16:16:40: 37000000 INFO @ Wed, 22 Jul 2020 16:16:42: 43000000 INFO @ Wed, 22 Jul 2020 16:16:43: 50000000 INFO @ Wed, 22 Jul 2020 16:16:46: 38000000 INFO @ Wed, 22 Jul 2020 16:16:48: 44000000 INFO @ Wed, 22 Jul 2020 16:16:49: 51000000 INFO @ Wed, 22 Jul 2020 16:16:51: 39000000 INFO @ Wed, 22 Jul 2020 16:16:53: 45000000 INFO @ Wed, 22 Jul 2020 16:16:54: 52000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 16:16:57: 40000000 INFO @ Wed, 22 Jul 2020 16:16:59: 46000000 INFO @ Wed, 22 Jul 2020 16:17:00: 53000000 INFO @ Wed, 22 Jul 2020 16:17:03: 41000000 INFO @ Wed, 22 Jul 2020 16:17:04: 47000000 INFO @ Wed, 22 Jul 2020 16:17:05: 54000000 INFO @ Wed, 22 Jul 2020 16:17:08: 42000000 INFO @ Wed, 22 Jul 2020 16:17:10: 48000000 INFO @ Wed, 22 Jul 2020 16:17:11: 55000000 INFO @ Wed, 22 Jul 2020 16:17:14: 43000000 INFO @ Wed, 22 Jul 2020 16:17:16: 49000000 INFO @ Wed, 22 Jul 2020 16:17:17: 56000000 INFO @ Wed, 22 Jul 2020 16:17:20: 44000000 INFO @ Wed, 22 Jul 2020 16:17:21: 50000000 INFO @ Wed, 22 Jul 2020 16:17:22: 57000000 INFO @ Wed, 22 Jul 2020 16:17:25: 45000000 INFO @ Wed, 22 Jul 2020 16:17:27: 51000000 INFO @ Wed, 22 Jul 2020 16:17:28: 58000000 INFO @ Wed, 22 Jul 2020 16:17:31: 46000000 INFO @ Wed, 22 Jul 2020 16:17:32: 52000000 INFO @ Wed, 22 Jul 2020 16:17:33: 59000000 INFO @ Wed, 22 Jul 2020 16:17:36: 47000000 INFO @ Wed, 22 Jul 2020 16:17:38: 53000000 INFO @ Wed, 22 Jul 2020 16:17:39: 60000000 INFO @ Wed, 22 Jul 2020 16:17:42: 48000000 INFO @ Wed, 22 Jul 2020 16:17:44: 54000000 INFO @ Wed, 22 Jul 2020 16:17:44: 61000000 INFO @ Wed, 22 Jul 2020 16:17:47: 49000000 INFO @ Wed, 22 Jul 2020 16:17:49: 55000000 INFO @ Wed, 22 Jul 2020 16:17:50: 62000000 INFO @ Wed, 22 Jul 2020 16:17:53: 50000000 INFO @ Wed, 22 Jul 2020 16:17:54: 56000000 INFO @ Wed, 22 Jul 2020 16:17:55: 63000000 INFO @ Wed, 22 Jul 2020 16:17:58: 51000000 INFO @ Wed, 22 Jul 2020 16:18:00: 57000000 INFO @ Wed, 22 Jul 2020 16:18:01: 64000000 INFO @ Wed, 22 Jul 2020 16:18:04: 52000000 INFO @ Wed, 22 Jul 2020 16:18:06: 58000000 INFO @ Wed, 22 Jul 2020 16:18:06: 65000000 INFO @ Wed, 22 Jul 2020 16:18:09: 53000000 INFO @ Wed, 22 Jul 2020 16:18:11: 59000000 INFO @ Wed, 22 Jul 2020 16:18:12: 66000000 INFO @ Wed, 22 Jul 2020 16:18:15: 54000000 INFO @ Wed, 22 Jul 2020 16:18:17: 60000000 INFO @ Wed, 22 Jul 2020 16:18:17: 67000000 INFO @ Wed, 22 Jul 2020 16:18:21: 55000000 INFO @ Wed, 22 Jul 2020 16:18:22: 61000000 INFO @ Wed, 22 Jul 2020 16:18:23: 68000000 INFO @ Wed, 22 Jul 2020 16:18:26: 56000000 INFO @ Wed, 22 Jul 2020 16:18:28: 62000000 INFO @ Wed, 22 Jul 2020 16:18:28: 69000000 INFO @ Wed, 22 Jul 2020 16:18:32: 57000000 INFO @ Wed, 22 Jul 2020 16:18:34: 70000000 INFO @ Wed, 22 Jul 2020 16:18:34: 63000000 INFO @ Wed, 22 Jul 2020 16:18:37: 58000000 INFO @ Wed, 22 Jul 2020 16:18:39: 71000000 INFO @ Wed, 22 Jul 2020 16:18:39: 64000000 INFO @ Wed, 22 Jul 2020 16:18:43: 59000000 INFO @ Wed, 22 Jul 2020 16:18:44: 72000000 INFO @ Wed, 22 Jul 2020 16:18:45: 65000000 INFO @ Wed, 22 Jul 2020 16:18:48: 60000000 INFO @ Wed, 22 Jul 2020 16:18:50: 73000000 INFO @ Wed, 22 Jul 2020 16:18:50: 66000000 INFO @ Wed, 22 Jul 2020 16:18:54: 61000000 INFO @ Wed, 22 Jul 2020 16:18:55: 74000000 INFO @ Wed, 22 Jul 2020 16:18:56: 67000000 INFO @ Wed, 22 Jul 2020 16:18:59: 62000000 INFO @ Wed, 22 Jul 2020 16:19:01: 75000000 INFO @ Wed, 22 Jul 2020 16:19:01: 68000000 INFO @ Wed, 22 Jul 2020 16:19:02: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:19:02: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:19:02: #1 total tags in treatment: 36638081 INFO @ Wed, 22 Jul 2020 16:19:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:19:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:19:03: #1 tags after filtering in treatment: 17870096 INFO @ Wed, 22 Jul 2020 16:19:03: #1 Redundant rate of treatment: 0.51 INFO @ Wed, 22 Jul 2020 16:19:03: #1 finished! INFO @ Wed, 22 Jul 2020 16:19:03: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:19:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:19:04: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:19:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:19:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:19:05: 63000000 INFO @ Wed, 22 Jul 2020 16:19:07: 69000000 INFO @ Wed, 22 Jul 2020 16:19:10: 64000000 INFO @ Wed, 22 Jul 2020 16:19:12: 70000000 INFO @ Wed, 22 Jul 2020 16:19:16: 65000000 INFO @ Wed, 22 Jul 2020 16:19:17: 71000000 INFO @ Wed, 22 Jul 2020 16:19:21: 66000000 INFO @ Wed, 22 Jul 2020 16:19:23: 72000000 INFO @ Wed, 22 Jul 2020 16:19:26: 67000000 INFO @ Wed, 22 Jul 2020 16:19:28: 73000000 INFO @ Wed, 22 Jul 2020 16:19:32: 68000000 INFO @ Wed, 22 Jul 2020 16:19:34: 74000000 INFO @ Wed, 22 Jul 2020 16:19:37: 69000000 INFO @ Wed, 22 Jul 2020 16:19:39: 75000000 INFO @ Wed, 22 Jul 2020 16:19:40: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:19:40: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:19:40: #1 total tags in treatment: 36638081 INFO @ Wed, 22 Jul 2020 16:19:40: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:19:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:19:41: #1 tags after filtering in treatment: 17870096 INFO @ Wed, 22 Jul 2020 16:19:41: #1 Redundant rate of treatment: 0.51 INFO @ Wed, 22 Jul 2020 16:19:41: #1 finished! INFO @ Wed, 22 Jul 2020 16:19:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:19:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:19:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:19:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:19:42: Process for pairing-model is terminated! INFO @ Wed, 22 Jul 2020 16:19:42: 70000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 16:19:48: 71000000 INFO @ Wed, 22 Jul 2020 16:19:53: 72000000 INFO @ Wed, 22 Jul 2020 16:19:58: 73000000 INFO @ Wed, 22 Jul 2020 16:20:03: 74000000 INFO @ Wed, 22 Jul 2020 16:20:08: 75000000 INFO @ Wed, 22 Jul 2020 16:20:09: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 16:20:09: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 16:20:09: #1 total tags in treatment: 36638081 INFO @ Wed, 22 Jul 2020 16:20:09: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 16:20:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 16:20:09: #1 tags after filtering in treatment: 17870096 INFO @ Wed, 22 Jul 2020 16:20:09: #1 Redundant rate of treatment: 0.51 INFO @ Wed, 22 Jul 2020 16:20:09: #1 finished! INFO @ Wed, 22 Jul 2020 16:20:09: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 16:20:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 16:20:11: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 16:20:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 16:20:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639491/SRX8639491.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling