Job ID = 7116516 SRX = SRX8639478 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:16:32 prefetch.2.10.7: 1) Downloading 'SRR12117042'... 2020-07-22T06:16:32 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:19:29 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:19:29 prefetch.2.10.7: 1) 'SRR12117042' was downloaded successfully 2020-07-22T06:19:29 prefetch.2.10.7: 'SRR12117042' has 0 unresolved dependencies Read 31805623 spots for SRR12117042/SRR12117042.sra Written 31805623 spots for SRR12117042/SRR12117042.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:45 31805623 reads; of these: 31805623 (100.00%) were paired; of these: 5124803 (16.11%) aligned concordantly 0 times 24612514 (77.38%) aligned concordantly exactly 1 time 2068306 (6.50%) aligned concordantly >1 times ---- 5124803 pairs aligned concordantly 0 times; of these: 835566 (16.30%) aligned discordantly 1 time ---- 4289237 pairs aligned 0 times concordantly or discordantly; of these: 8578474 mates make up the pairs; of these: 8155027 (95.06%) aligned 0 times 305081 (3.56%) aligned exactly 1 time 118366 (1.38%) aligned >1 times 87.18% overall alignment rate Time searching: 00:14:45 Overall time: 00:14:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 322893 / 27448825 = 0.0118 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:47:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:47:34: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:47:34: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:47:38: 1000000 INFO @ Wed, 22 Jul 2020 15:47:43: 2000000 INFO @ Wed, 22 Jul 2020 15:47:47: 3000000 INFO @ Wed, 22 Jul 2020 15:47:52: 4000000 INFO @ Wed, 22 Jul 2020 15:47:56: 5000000 INFO @ Wed, 22 Jul 2020 15:48:00: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:48:03: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:48:03: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:48:05: 7000000 INFO @ Wed, 22 Jul 2020 15:48:09: 1000000 INFO @ Wed, 22 Jul 2020 15:48:09: 8000000 INFO @ Wed, 22 Jul 2020 15:48:14: 9000000 INFO @ Wed, 22 Jul 2020 15:48:14: 2000000 INFO @ Wed, 22 Jul 2020 15:48:18: 10000000 INFO @ Wed, 22 Jul 2020 15:48:19: 3000000 INFO @ Wed, 22 Jul 2020 15:48:22: 11000000 INFO @ Wed, 22 Jul 2020 15:48:24: 4000000 INFO @ Wed, 22 Jul 2020 15:48:27: 12000000 INFO @ Wed, 22 Jul 2020 15:48:29: 5000000 INFO @ Wed, 22 Jul 2020 15:48:31: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:48:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:48:33: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:48:33: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:48:34: 6000000 INFO @ Wed, 22 Jul 2020 15:48:36: 14000000 INFO @ Wed, 22 Jul 2020 15:48:39: 1000000 INFO @ Wed, 22 Jul 2020 15:48:39: 7000000 INFO @ Wed, 22 Jul 2020 15:48:40: 15000000 INFO @ Wed, 22 Jul 2020 15:48:44: 2000000 INFO @ Wed, 22 Jul 2020 15:48:44: 8000000 INFO @ Wed, 22 Jul 2020 15:48:45: 16000000 INFO @ Wed, 22 Jul 2020 15:48:49: 3000000 INFO @ Wed, 22 Jul 2020 15:48:49: 17000000 INFO @ Wed, 22 Jul 2020 15:48:49: 9000000 INFO @ Wed, 22 Jul 2020 15:48:54: 18000000 INFO @ Wed, 22 Jul 2020 15:48:54: 10000000 INFO @ Wed, 22 Jul 2020 15:48:54: 4000000 INFO @ Wed, 22 Jul 2020 15:48:58: 19000000 INFO @ Wed, 22 Jul 2020 15:48:59: 11000000 INFO @ Wed, 22 Jul 2020 15:48:59: 5000000 INFO @ Wed, 22 Jul 2020 15:49:03: 20000000 INFO @ Wed, 22 Jul 2020 15:49:04: 12000000 INFO @ Wed, 22 Jul 2020 15:49:05: 6000000 INFO @ Wed, 22 Jul 2020 15:49:07: 21000000 INFO @ Wed, 22 Jul 2020 15:49:09: 13000000 INFO @ Wed, 22 Jul 2020 15:49:10: 7000000 INFO @ Wed, 22 Jul 2020 15:49:12: 22000000 INFO @ Wed, 22 Jul 2020 15:49:14: 14000000 INFO @ Wed, 22 Jul 2020 15:49:15: 8000000 INFO @ Wed, 22 Jul 2020 15:49:16: 23000000 INFO @ Wed, 22 Jul 2020 15:49:19: 15000000 INFO @ Wed, 22 Jul 2020 15:49:20: 9000000 INFO @ Wed, 22 Jul 2020 15:49:21: 24000000 INFO @ Wed, 22 Jul 2020 15:49:24: 16000000 INFO @ Wed, 22 Jul 2020 15:49:25: 10000000 INFO @ Wed, 22 Jul 2020 15:49:25: 25000000 INFO @ Wed, 22 Jul 2020 15:49:29: 17000000 INFO @ Wed, 22 Jul 2020 15:49:30: 26000000 INFO @ Wed, 22 Jul 2020 15:49:30: 11000000 INFO @ Wed, 22 Jul 2020 15:49:34: 27000000 INFO @ Wed, 22 Jul 2020 15:49:34: 18000000 INFO @ Wed, 22 Jul 2020 15:49:35: 12000000 INFO @ Wed, 22 Jul 2020 15:49:38: 28000000 INFO @ Wed, 22 Jul 2020 15:49:39: 19000000 INFO @ Wed, 22 Jul 2020 15:49:40: 13000000 INFO @ Wed, 22 Jul 2020 15:49:43: 29000000 INFO @ Wed, 22 Jul 2020 15:49:44: 20000000 INFO @ Wed, 22 Jul 2020 15:49:45: 14000000 INFO @ Wed, 22 Jul 2020 15:49:47: 30000000 INFO @ Wed, 22 Jul 2020 15:49:49: 21000000 INFO @ Wed, 22 Jul 2020 15:49:50: 15000000 INFO @ Wed, 22 Jul 2020 15:49:52: 31000000 INFO @ Wed, 22 Jul 2020 15:49:54: 22000000 INFO @ Wed, 22 Jul 2020 15:49:55: 16000000 INFO @ Wed, 22 Jul 2020 15:49:56: 32000000 INFO @ Wed, 22 Jul 2020 15:49:58: 23000000 INFO @ Wed, 22 Jul 2020 15:50:00: 17000000 INFO @ Wed, 22 Jul 2020 15:50:01: 33000000 INFO @ Wed, 22 Jul 2020 15:50:03: 24000000 INFO @ Wed, 22 Jul 2020 15:50:05: 18000000 INFO @ Wed, 22 Jul 2020 15:50:05: 34000000 INFO @ Wed, 22 Jul 2020 15:50:08: 25000000 INFO @ Wed, 22 Jul 2020 15:50:10: 35000000 INFO @ Wed, 22 Jul 2020 15:50:10: 19000000 INFO @ Wed, 22 Jul 2020 15:50:13: 26000000 INFO @ Wed, 22 Jul 2020 15:50:14: 36000000 INFO @ Wed, 22 Jul 2020 15:50:15: 20000000 INFO @ Wed, 22 Jul 2020 15:50:18: 27000000 INFO @ Wed, 22 Jul 2020 15:50:18: 37000000 INFO @ Wed, 22 Jul 2020 15:50:20: 21000000 INFO @ Wed, 22 Jul 2020 15:50:23: 38000000 INFO @ Wed, 22 Jul 2020 15:50:23: 28000000 INFO @ Wed, 22 Jul 2020 15:50:25: 22000000 INFO @ Wed, 22 Jul 2020 15:50:27: 39000000 INFO @ Wed, 22 Jul 2020 15:50:28: 29000000 INFO @ Wed, 22 Jul 2020 15:50:30: 23000000 INFO @ Wed, 22 Jul 2020 15:50:32: 40000000 INFO @ Wed, 22 Jul 2020 15:50:33: 30000000 INFO @ Wed, 22 Jul 2020 15:50:35: 24000000 INFO @ Wed, 22 Jul 2020 15:50:36: 41000000 INFO @ Wed, 22 Jul 2020 15:50:38: 31000000 INFO @ Wed, 22 Jul 2020 15:50:40: 25000000 INFO @ Wed, 22 Jul 2020 15:50:41: 42000000 INFO @ Wed, 22 Jul 2020 15:50:42: 32000000 INFO @ Wed, 22 Jul 2020 15:50:45: 26000000 INFO @ Wed, 22 Jul 2020 15:50:45: 43000000 INFO @ Wed, 22 Jul 2020 15:50:47: 33000000 INFO @ Wed, 22 Jul 2020 15:50:50: 27000000 INFO @ Wed, 22 Jul 2020 15:50:50: 44000000 INFO @ Wed, 22 Jul 2020 15:50:52: 34000000 INFO @ Wed, 22 Jul 2020 15:50:54: 45000000 INFO @ Wed, 22 Jul 2020 15:50:54: 28000000 INFO @ Wed, 22 Jul 2020 15:50:57: 35000000 INFO @ Wed, 22 Jul 2020 15:50:58: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:50:59: 29000000 INFO @ Wed, 22 Jul 2020 15:51:02: 36000000 INFO @ Wed, 22 Jul 2020 15:51:03: 47000000 INFO @ Wed, 22 Jul 2020 15:51:04: 30000000 INFO @ Wed, 22 Jul 2020 15:51:07: 37000000 INFO @ Wed, 22 Jul 2020 15:51:07: 48000000 INFO @ Wed, 22 Jul 2020 15:51:09: 31000000 INFO @ Wed, 22 Jul 2020 15:51:12: 38000000 INFO @ Wed, 22 Jul 2020 15:51:12: 49000000 INFO @ Wed, 22 Jul 2020 15:51:14: 32000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:51:16: 50000000 INFO @ Wed, 22 Jul 2020 15:51:17: 39000000 INFO @ Wed, 22 Jul 2020 15:51:19: 33000000 INFO @ Wed, 22 Jul 2020 15:51:21: 51000000 INFO @ Wed, 22 Jul 2020 15:51:21: 40000000 INFO @ Wed, 22 Jul 2020 15:51:24: 34000000 INFO @ Wed, 22 Jul 2020 15:51:25: 52000000 INFO @ Wed, 22 Jul 2020 15:51:26: 41000000 INFO @ Wed, 22 Jul 2020 15:51:29: 35000000 INFO @ Wed, 22 Jul 2020 15:51:30: 53000000 INFO @ Wed, 22 Jul 2020 15:51:31: 42000000 INFO @ Wed, 22 Jul 2020 15:51:34: 36000000 INFO @ Wed, 22 Jul 2020 15:51:34: 54000000 INFO @ Wed, 22 Jul 2020 15:51:36: 43000000 INFO @ Wed, 22 Jul 2020 15:51:38: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:51:38: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:51:38: #1 total tags in treatment: 26358424 INFO @ Wed, 22 Jul 2020 15:51:38: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:51:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:51:39: #1 tags after filtering in treatment: 13973172 INFO @ Wed, 22 Jul 2020 15:51:39: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:51:39: #1 finished! INFO @ Wed, 22 Jul 2020 15:51:39: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:51:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:51:39: 37000000 INFO @ Wed, 22 Jul 2020 15:51:39: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:51:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:51:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:51:41: 44000000 INFO @ Wed, 22 Jul 2020 15:51:43: 38000000 INFO @ Wed, 22 Jul 2020 15:51:46: 45000000 INFO @ Wed, 22 Jul 2020 15:51:48: 39000000 INFO @ Wed, 22 Jul 2020 15:51:51: 46000000 INFO @ Wed, 22 Jul 2020 15:51:53: 40000000 INFO @ Wed, 22 Jul 2020 15:51:56: 47000000 INFO @ Wed, 22 Jul 2020 15:51:58: 41000000 INFO @ Wed, 22 Jul 2020 15:52:00: 48000000 INFO @ Wed, 22 Jul 2020 15:52:03: 42000000 INFO @ Wed, 22 Jul 2020 15:52:05: 49000000 INFO @ Wed, 22 Jul 2020 15:52:08: 43000000 INFO @ Wed, 22 Jul 2020 15:52:10: 50000000 INFO @ Wed, 22 Jul 2020 15:52:13: 44000000 INFO @ Wed, 22 Jul 2020 15:52:15: 51000000 INFO @ Wed, 22 Jul 2020 15:52:18: 45000000 INFO @ Wed, 22 Jul 2020 15:52:20: 52000000 INFO @ Wed, 22 Jul 2020 15:52:23: 46000000 INFO @ Wed, 22 Jul 2020 15:52:25: 53000000 INFO @ Wed, 22 Jul 2020 15:52:27: 47000000 INFO @ Wed, 22 Jul 2020 15:52:29: 54000000 INFO @ Wed, 22 Jul 2020 15:52:32: 48000000 INFO @ Wed, 22 Jul 2020 15:52:34: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:52:34: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:52:34: #1 total tags in treatment: 26358424 INFO @ Wed, 22 Jul 2020 15:52:34: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:52:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:52:34: #1 tags after filtering in treatment: 13973172 INFO @ Wed, 22 Jul 2020 15:52:34: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:52:34: #1 finished! INFO @ Wed, 22 Jul 2020 15:52:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:52:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:52:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:52:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:52:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:52:37: 49000000 INFO @ Wed, 22 Jul 2020 15:52:42: 50000000 INFO @ Wed, 22 Jul 2020 15:52:47: 51000000 INFO @ Wed, 22 Jul 2020 15:52:52: 52000000 INFO @ Wed, 22 Jul 2020 15:52:57: 53000000 INFO @ Wed, 22 Jul 2020 15:53:01: 54000000 INFO @ Wed, 22 Jul 2020 15:53:06: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:53:06: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:53:06: #1 total tags in treatment: 26358424 INFO @ Wed, 22 Jul 2020 15:53:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:53:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:53:06: #1 tags after filtering in treatment: 13973172 INFO @ Wed, 22 Jul 2020 15:53:06: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:53:06: #1 finished! INFO @ Wed, 22 Jul 2020 15:53:06: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:53:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:53:07: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:53:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:53:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639478/SRX8639478.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling