Job ID = 7116513 SRX = SRX8639477 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:16:18 prefetch.2.10.7: 1) Downloading 'SRR12117041'... 2020-07-22T06:16:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:18:54 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:18:54 prefetch.2.10.7: 1) 'SRR12117041' was downloaded successfully 2020-07-22T06:18:54 prefetch.2.10.7: 'SRR12117041' has 0 unresolved dependencies Read 32610615 spots for SRR12117041/SRR12117041.sra Written 32610615 spots for SRR12117041/SRR12117041.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:44 32610615 reads; of these: 32610615 (100.00%) were paired; of these: 6320460 (19.38%) aligned concordantly 0 times 24305365 (74.53%) aligned concordantly exactly 1 time 1984790 (6.09%) aligned concordantly >1 times ---- 6320460 pairs aligned concordantly 0 times; of these: 937369 (14.83%) aligned discordantly 1 time ---- 5383091 pairs aligned 0 times concordantly or discordantly; of these: 10766182 mates make up the pairs; of these: 10269284 (95.38%) aligned 0 times 368842 (3.43%) aligned exactly 1 time 128056 (1.19%) aligned >1 times 84.25% overall alignment rate Time searching: 00:14:44 Overall time: 00:14:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 328431 / 27159740 = 0.0121 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:47:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:47:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:47:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:47:16: 1000000 INFO @ Wed, 22 Jul 2020 15:47:20: 2000000 INFO @ Wed, 22 Jul 2020 15:47:25: 3000000 INFO @ Wed, 22 Jul 2020 15:47:29: 4000000 INFO @ Wed, 22 Jul 2020 15:47:34: 5000000 INFO @ Wed, 22 Jul 2020 15:47:38: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:47:41: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:47:41: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:47:43: 7000000 INFO @ Wed, 22 Jul 2020 15:47:46: 1000000 INFO @ Wed, 22 Jul 2020 15:47:48: 8000000 INFO @ Wed, 22 Jul 2020 15:47:51: 2000000 INFO @ Wed, 22 Jul 2020 15:47:53: 9000000 INFO @ Wed, 22 Jul 2020 15:47:56: 3000000 INFO @ Wed, 22 Jul 2020 15:47:57: 10000000 INFO @ Wed, 22 Jul 2020 15:48:01: 4000000 INFO @ Wed, 22 Jul 2020 15:48:02: 11000000 INFO @ Wed, 22 Jul 2020 15:48:06: 5000000 INFO @ Wed, 22 Jul 2020 15:48:07: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:48:11: 6000000 INFO @ Wed, 22 Jul 2020 15:48:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:48:11: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:48:11: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:48:12: 13000000 INFO @ Wed, 22 Jul 2020 15:48:16: 7000000 INFO @ Wed, 22 Jul 2020 15:48:16: 1000000 INFO @ Wed, 22 Jul 2020 15:48:17: 14000000 INFO @ Wed, 22 Jul 2020 15:48:20: 8000000 INFO @ Wed, 22 Jul 2020 15:48:21: 2000000 INFO @ Wed, 22 Jul 2020 15:48:22: 15000000 INFO @ Wed, 22 Jul 2020 15:48:25: 9000000 INFO @ Wed, 22 Jul 2020 15:48:26: 3000000 INFO @ Wed, 22 Jul 2020 15:48:27: 16000000 INFO @ Wed, 22 Jul 2020 15:48:30: 10000000 INFO @ Wed, 22 Jul 2020 15:48:31: 4000000 INFO @ Wed, 22 Jul 2020 15:48:32: 17000000 INFO @ Wed, 22 Jul 2020 15:48:35: 11000000 INFO @ Wed, 22 Jul 2020 15:48:37: 5000000 INFO @ Wed, 22 Jul 2020 15:48:37: 18000000 INFO @ Wed, 22 Jul 2020 15:48:40: 12000000 INFO @ Wed, 22 Jul 2020 15:48:42: 6000000 INFO @ Wed, 22 Jul 2020 15:48:42: 19000000 INFO @ Wed, 22 Jul 2020 15:48:45: 13000000 INFO @ Wed, 22 Jul 2020 15:48:47: 7000000 INFO @ Wed, 22 Jul 2020 15:48:47: 20000000 INFO @ Wed, 22 Jul 2020 15:48:50: 14000000 INFO @ Wed, 22 Jul 2020 15:48:52: 8000000 INFO @ Wed, 22 Jul 2020 15:48:53: 21000000 INFO @ Wed, 22 Jul 2020 15:48:55: 15000000 INFO @ Wed, 22 Jul 2020 15:48:56: 9000000 INFO @ Wed, 22 Jul 2020 15:48:59: 22000000 INFO @ Wed, 22 Jul 2020 15:49:00: 16000000 INFO @ Wed, 22 Jul 2020 15:49:01: 10000000 INFO @ Wed, 22 Jul 2020 15:49:05: 23000000 INFO @ Wed, 22 Jul 2020 15:49:05: 17000000 INFO @ Wed, 22 Jul 2020 15:49:06: 11000000 INFO @ Wed, 22 Jul 2020 15:49:10: 18000000 INFO @ Wed, 22 Jul 2020 15:49:11: 24000000 INFO @ Wed, 22 Jul 2020 15:49:11: 12000000 INFO @ Wed, 22 Jul 2020 15:49:15: 19000000 INFO @ Wed, 22 Jul 2020 15:49:16: 13000000 INFO @ Wed, 22 Jul 2020 15:49:16: 25000000 INFO @ Wed, 22 Jul 2020 15:49:20: 20000000 INFO @ Wed, 22 Jul 2020 15:49:21: 14000000 INFO @ Wed, 22 Jul 2020 15:49:21: 26000000 INFO @ Wed, 22 Jul 2020 15:49:26: 15000000 INFO @ Wed, 22 Jul 2020 15:49:26: 21000000 INFO @ Wed, 22 Jul 2020 15:49:26: 27000000 INFO @ Wed, 22 Jul 2020 15:49:31: 16000000 INFO @ Wed, 22 Jul 2020 15:49:31: 28000000 INFO @ Wed, 22 Jul 2020 15:49:32: 22000000 INFO @ Wed, 22 Jul 2020 15:49:36: 29000000 INFO @ Wed, 22 Jul 2020 15:49:36: 17000000 INFO @ Wed, 22 Jul 2020 15:49:38: 23000000 INFO @ Wed, 22 Jul 2020 15:49:41: 30000000 INFO @ Wed, 22 Jul 2020 15:49:41: 18000000 INFO @ Wed, 22 Jul 2020 15:49:43: 24000000 INFO @ Wed, 22 Jul 2020 15:49:45: 31000000 INFO @ Wed, 22 Jul 2020 15:49:46: 19000000 INFO @ Wed, 22 Jul 2020 15:49:49: 25000000 INFO @ Wed, 22 Jul 2020 15:49:50: 32000000 INFO @ Wed, 22 Jul 2020 15:49:51: 20000000 INFO @ Wed, 22 Jul 2020 15:49:54: 26000000 INFO @ Wed, 22 Jul 2020 15:49:55: 33000000 INFO @ Wed, 22 Jul 2020 15:49:57: 21000000 INFO @ Wed, 22 Jul 2020 15:49:59: 27000000 INFO @ Wed, 22 Jul 2020 15:50:00: 34000000 INFO @ Wed, 22 Jul 2020 15:50:03: 22000000 INFO @ Wed, 22 Jul 2020 15:50:04: 28000000 INFO @ Wed, 22 Jul 2020 15:50:05: 35000000 INFO @ Wed, 22 Jul 2020 15:50:09: 23000000 INFO @ Wed, 22 Jul 2020 15:50:09: 29000000 INFO @ Wed, 22 Jul 2020 15:50:10: 36000000 INFO @ Wed, 22 Jul 2020 15:50:14: 30000000 INFO @ Wed, 22 Jul 2020 15:50:14: 24000000 INFO @ Wed, 22 Jul 2020 15:50:15: 37000000 INFO @ Wed, 22 Jul 2020 15:50:19: 31000000 INFO @ Wed, 22 Jul 2020 15:50:20: 25000000 INFO @ Wed, 22 Jul 2020 15:50:20: 38000000 INFO @ Wed, 22 Jul 2020 15:50:24: 32000000 INFO @ Wed, 22 Jul 2020 15:50:25: 26000000 INFO @ Wed, 22 Jul 2020 15:50:25: 39000000 INFO @ Wed, 22 Jul 2020 15:50:29: 33000000 INFO @ Wed, 22 Jul 2020 15:50:30: 27000000 INFO @ Wed, 22 Jul 2020 15:50:30: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:50:34: 34000000 INFO @ Wed, 22 Jul 2020 15:50:35: 28000000 INFO @ Wed, 22 Jul 2020 15:50:36: 41000000 INFO @ Wed, 22 Jul 2020 15:50:39: 35000000 INFO @ Wed, 22 Jul 2020 15:50:40: 29000000 INFO @ Wed, 22 Jul 2020 15:50:41: 42000000 INFO @ Wed, 22 Jul 2020 15:50:44: 36000000 INFO @ Wed, 22 Jul 2020 15:50:45: 30000000 INFO @ Wed, 22 Jul 2020 15:50:46: 43000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:50:49: 37000000 INFO @ Wed, 22 Jul 2020 15:50:50: 31000000 INFO @ Wed, 22 Jul 2020 15:50:50: 44000000 INFO @ Wed, 22 Jul 2020 15:50:54: 38000000 INFO @ Wed, 22 Jul 2020 15:50:55: 32000000 INFO @ Wed, 22 Jul 2020 15:50:55: 45000000 INFO @ Wed, 22 Jul 2020 15:50:59: 39000000 INFO @ Wed, 22 Jul 2020 15:51:00: 33000000 INFO @ Wed, 22 Jul 2020 15:51:00: 46000000 INFO @ Wed, 22 Jul 2020 15:51:04: 40000000 INFO @ Wed, 22 Jul 2020 15:51:05: 34000000 INFO @ Wed, 22 Jul 2020 15:51:06: 47000000 INFO @ Wed, 22 Jul 2020 15:51:09: 41000000 INFO @ Wed, 22 Jul 2020 15:51:10: 35000000 INFO @ Wed, 22 Jul 2020 15:51:10: 48000000 INFO @ Wed, 22 Jul 2020 15:51:14: 42000000 INFO @ Wed, 22 Jul 2020 15:51:15: 36000000 INFO @ Wed, 22 Jul 2020 15:51:15: 49000000 INFO @ Wed, 22 Jul 2020 15:51:19: 43000000 INFO @ Wed, 22 Jul 2020 15:51:20: 37000000 INFO @ Wed, 22 Jul 2020 15:51:20: 50000000 INFO @ Wed, 22 Jul 2020 15:51:24: 44000000 INFO @ Wed, 22 Jul 2020 15:51:25: 51000000 INFO @ Wed, 22 Jul 2020 15:51:25: 38000000 INFO @ Wed, 22 Jul 2020 15:51:29: 45000000 INFO @ Wed, 22 Jul 2020 15:51:30: 52000000 INFO @ Wed, 22 Jul 2020 15:51:30: 39000000 INFO @ Wed, 22 Jul 2020 15:51:34: 46000000 INFO @ Wed, 22 Jul 2020 15:51:35: 53000000 INFO @ Wed, 22 Jul 2020 15:51:35: 40000000 INFO @ Wed, 22 Jul 2020 15:51:39: 47000000 INFO @ Wed, 22 Jul 2020 15:51:40: 54000000 INFO @ Wed, 22 Jul 2020 15:51:41: 41000000 INFO @ Wed, 22 Jul 2020 15:51:42: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:51:42: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:51:42: #1 total tags in treatment: 25962186 INFO @ Wed, 22 Jul 2020 15:51:42: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:51:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:51:42: #1 tags after filtering in treatment: 13818510 INFO @ Wed, 22 Jul 2020 15:51:42: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:51:42: #1 finished! INFO @ Wed, 22 Jul 2020 15:51:42: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:51:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:51:43: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:51:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:51:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:51:44: 48000000 INFO @ Wed, 22 Jul 2020 15:51:46: 42000000 INFO @ Wed, 22 Jul 2020 15:51:49: 49000000 INFO @ Wed, 22 Jul 2020 15:51:51: 43000000 INFO @ Wed, 22 Jul 2020 15:51:54: 50000000 INFO @ Wed, 22 Jul 2020 15:51:56: 44000000 INFO @ Wed, 22 Jul 2020 15:51:59: 51000000 INFO @ Wed, 22 Jul 2020 15:52:01: 45000000 INFO @ Wed, 22 Jul 2020 15:52:04: 52000000 INFO @ Wed, 22 Jul 2020 15:52:06: 46000000 INFO @ Wed, 22 Jul 2020 15:52:09: 53000000 INFO @ Wed, 22 Jul 2020 15:52:11: 47000000 INFO @ Wed, 22 Jul 2020 15:52:14: 54000000 INFO @ Wed, 22 Jul 2020 15:52:15: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:52:15: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:52:15: #1 total tags in treatment: 25962186 INFO @ Wed, 22 Jul 2020 15:52:15: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:52:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:52:16: 48000000 INFO @ Wed, 22 Jul 2020 15:52:16: #1 tags after filtering in treatment: 13818510 INFO @ Wed, 22 Jul 2020 15:52:16: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:52:16: #1 finished! INFO @ Wed, 22 Jul 2020 15:52:16: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:52:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:52:16: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:52:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:52:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:52:20: 49000000 INFO @ Wed, 22 Jul 2020 15:52:25: 50000000 INFO @ Wed, 22 Jul 2020 15:52:30: 51000000 INFO @ Wed, 22 Jul 2020 15:52:34: 52000000 INFO @ Wed, 22 Jul 2020 15:52:39: 53000000 INFO @ Wed, 22 Jul 2020 15:52:43: 54000000 INFO @ Wed, 22 Jul 2020 15:52:45: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:52:45: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:52:45: #1 total tags in treatment: 25962186 INFO @ Wed, 22 Jul 2020 15:52:45: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:52:45: #1 tags after filtering in treatment: 13818510 INFO @ Wed, 22 Jul 2020 15:52:45: #1 Redundant rate of treatment: 0.47 INFO @ Wed, 22 Jul 2020 15:52:45: #1 finished! INFO @ Wed, 22 Jul 2020 15:52:45: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:52:46: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:52:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:52:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639477/SRX8639477.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling