Job ID = 7116411 SRX = SRX8639476 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-07-22T06:15:59 prefetch.2.10.7: 1) Downloading 'SRR12117040'... 2020-07-22T06:15:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-22T06:19:31 prefetch.2.10.7: HTTPS download succeed 2020-07-22T06:19:31 prefetch.2.10.7: 1) 'SRR12117040' was downloaded successfully 2020-07-22T06:19:31 prefetch.2.10.7: 'SRR12117040' has 0 unresolved dependencies Read 34186863 spots for SRR12117040/SRR12117040.sra Written 34186863 spots for SRR12117040/SRR12117040.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:47 34186863 reads; of these: 34186863 (100.00%) were paired; of these: 5387016 (15.76%) aligned concordantly 0 times 26639167 (77.92%) aligned concordantly exactly 1 time 2160680 (6.32%) aligned concordantly >1 times ---- 5387016 pairs aligned concordantly 0 times; of these: 1049680 (19.49%) aligned discordantly 1 time ---- 4337336 pairs aligned 0 times concordantly or discordantly; of these: 8674672 mates make up the pairs; of these: 8139743 (93.83%) aligned 0 times 389603 (4.49%) aligned exactly 1 time 145326 (1.68%) aligned >1 times 88.10% overall alignment rate Time searching: 00:15:47 Overall time: 00:15:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 356762 / 29781726 = 0.0120 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:50:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:50:24: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:50:24: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:50:30: 1000000 INFO @ Wed, 22 Jul 2020 15:50:35: 2000000 INFO @ Wed, 22 Jul 2020 15:50:40: 3000000 INFO @ Wed, 22 Jul 2020 15:50:46: 4000000 INFO @ Wed, 22 Jul 2020 15:50:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:50:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:50:54: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:50:54: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:50:56: 6000000 INFO @ Wed, 22 Jul 2020 15:50:59: 1000000 INFO @ Wed, 22 Jul 2020 15:51:02: 7000000 INFO @ Wed, 22 Jul 2020 15:51:04: 2000000 INFO @ Wed, 22 Jul 2020 15:51:08: 8000000 INFO @ Wed, 22 Jul 2020 15:51:09: 3000000 INFO @ Wed, 22 Jul 2020 15:51:14: 9000000 INFO @ Wed, 22 Jul 2020 15:51:15: 4000000 INFO @ Wed, 22 Jul 2020 15:51:19: 10000000 INFO @ Wed, 22 Jul 2020 15:51:20: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 15:51:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 15:51:25: #1 read tag files... INFO @ Wed, 22 Jul 2020 15:51:25: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 15:51:25: 6000000 INFO @ Wed, 22 Jul 2020 15:51:25: 11000000 INFO @ Wed, 22 Jul 2020 15:51:30: 7000000 INFO @ Wed, 22 Jul 2020 15:51:31: 1000000 INFO @ Wed, 22 Jul 2020 15:51:31: 12000000 INFO @ Wed, 22 Jul 2020 15:51:36: 8000000 INFO @ Wed, 22 Jul 2020 15:51:36: 2000000 INFO @ Wed, 22 Jul 2020 15:51:36: 13000000 INFO @ Wed, 22 Jul 2020 15:51:41: 9000000 INFO @ Wed, 22 Jul 2020 15:51:42: 3000000 INFO @ Wed, 22 Jul 2020 15:51:42: 14000000 INFO @ Wed, 22 Jul 2020 15:51:46: 10000000 INFO @ Wed, 22 Jul 2020 15:51:48: 4000000 INFO @ Wed, 22 Jul 2020 15:51:48: 15000000 INFO @ Wed, 22 Jul 2020 15:51:52: 11000000 INFO @ Wed, 22 Jul 2020 15:51:53: 16000000 INFO @ Wed, 22 Jul 2020 15:51:53: 5000000 INFO @ Wed, 22 Jul 2020 15:51:57: 12000000 INFO @ Wed, 22 Jul 2020 15:51:59: 17000000 INFO @ Wed, 22 Jul 2020 15:51:59: 6000000 INFO @ Wed, 22 Jul 2020 15:52:02: 13000000 INFO @ Wed, 22 Jul 2020 15:52:04: 18000000 INFO @ Wed, 22 Jul 2020 15:52:05: 7000000 INFO @ Wed, 22 Jul 2020 15:52:08: 14000000 INFO @ Wed, 22 Jul 2020 15:52:10: 8000000 INFO @ Wed, 22 Jul 2020 15:52:10: 19000000 INFO @ Wed, 22 Jul 2020 15:52:13: 15000000 INFO @ Wed, 22 Jul 2020 15:52:15: 20000000 INFO @ Wed, 22 Jul 2020 15:52:15: 9000000 INFO @ Wed, 22 Jul 2020 15:52:18: 16000000 INFO @ Wed, 22 Jul 2020 15:52:21: 21000000 INFO @ Wed, 22 Jul 2020 15:52:21: 10000000 INFO @ Wed, 22 Jul 2020 15:52:23: 17000000 INFO @ Wed, 22 Jul 2020 15:52:27: 22000000 INFO @ Wed, 22 Jul 2020 15:52:27: 11000000 INFO @ Wed, 22 Jul 2020 15:52:29: 18000000 INFO @ Wed, 22 Jul 2020 15:52:32: 23000000 INFO @ Wed, 22 Jul 2020 15:52:33: 12000000 INFO @ Wed, 22 Jul 2020 15:52:34: 19000000 INFO @ Wed, 22 Jul 2020 15:52:38: 24000000 INFO @ Wed, 22 Jul 2020 15:52:39: 13000000 INFO @ Wed, 22 Jul 2020 15:52:39: 20000000 INFO @ Wed, 22 Jul 2020 15:52:43: 25000000 INFO @ Wed, 22 Jul 2020 15:52:44: 14000000 INFO @ Wed, 22 Jul 2020 15:52:44: 21000000 INFO @ Wed, 22 Jul 2020 15:52:49: 26000000 INFO @ Wed, 22 Jul 2020 15:52:50: 22000000 INFO @ Wed, 22 Jul 2020 15:52:50: 15000000 INFO @ Wed, 22 Jul 2020 15:52:54: 27000000 INFO @ Wed, 22 Jul 2020 15:52:55: 23000000 INFO @ Wed, 22 Jul 2020 15:52:56: 16000000 INFO @ Wed, 22 Jul 2020 15:53:00: 28000000 INFO @ Wed, 22 Jul 2020 15:53:00: 24000000 INFO @ Wed, 22 Jul 2020 15:53:02: 17000000 INFO @ Wed, 22 Jul 2020 15:53:05: 25000000 INFO @ Wed, 22 Jul 2020 15:53:06: 29000000 INFO @ Wed, 22 Jul 2020 15:53:08: 18000000 INFO @ Wed, 22 Jul 2020 15:53:10: 26000000 INFO @ Wed, 22 Jul 2020 15:53:11: 30000000 INFO @ Wed, 22 Jul 2020 15:53:14: 19000000 INFO @ Wed, 22 Jul 2020 15:53:16: 27000000 INFO @ Wed, 22 Jul 2020 15:53:17: 31000000 INFO @ Wed, 22 Jul 2020 15:53:20: 20000000 INFO @ Wed, 22 Jul 2020 15:53:21: 28000000 INFO @ Wed, 22 Jul 2020 15:53:22: 32000000 INFO @ Wed, 22 Jul 2020 15:53:25: 21000000 INFO @ Wed, 22 Jul 2020 15:53:26: 29000000 INFO @ Wed, 22 Jul 2020 15:53:28: 33000000 INFO @ Wed, 22 Jul 2020 15:53:31: 22000000 INFO @ Wed, 22 Jul 2020 15:53:31: 30000000 INFO @ Wed, 22 Jul 2020 15:53:33: 34000000 INFO @ Wed, 22 Jul 2020 15:53:37: 31000000 INFO @ Wed, 22 Jul 2020 15:53:37: 23000000 INFO @ Wed, 22 Jul 2020 15:53:39: 35000000 INFO @ Wed, 22 Jul 2020 15:53:42: 32000000 INFO @ Wed, 22 Jul 2020 15:53:43: 24000000 INFO @ Wed, 22 Jul 2020 15:53:44: 36000000 INFO @ Wed, 22 Jul 2020 15:53:47: 33000000 INFO @ Wed, 22 Jul 2020 15:53:49: 25000000 INFO @ Wed, 22 Jul 2020 15:53:50: 37000000 INFO @ Wed, 22 Jul 2020 15:53:52: 34000000 INFO @ Wed, 22 Jul 2020 15:53:55: 26000000 INFO @ Wed, 22 Jul 2020 15:53:56: 38000000 INFO @ Wed, 22 Jul 2020 15:53:57: 35000000 INFO @ Wed, 22 Jul 2020 15:54:00: 27000000 INFO @ Wed, 22 Jul 2020 15:54:01: 39000000 INFO @ Wed, 22 Jul 2020 15:54:03: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 15:54:06: 28000000 INFO @ Wed, 22 Jul 2020 15:54:07: 40000000 INFO @ Wed, 22 Jul 2020 15:54:08: 37000000 INFO @ Wed, 22 Jul 2020 15:54:11: 29000000 INFO @ Wed, 22 Jul 2020 15:54:12: 41000000 INFO @ Wed, 22 Jul 2020 15:54:13: 38000000 INFO @ Wed, 22 Jul 2020 15:54:17: 30000000 INFO @ Wed, 22 Jul 2020 15:54:17: 42000000 INFO @ Wed, 22 Jul 2020 15:54:18: 39000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 15:54:23: 31000000 INFO @ Wed, 22 Jul 2020 15:54:23: 43000000 INFO @ Wed, 22 Jul 2020 15:54:24: 40000000 INFO @ Wed, 22 Jul 2020 15:54:29: 44000000 INFO @ Wed, 22 Jul 2020 15:54:29: 32000000 INFO @ Wed, 22 Jul 2020 15:54:29: 41000000 INFO @ Wed, 22 Jul 2020 15:54:34: 45000000 INFO @ Wed, 22 Jul 2020 15:54:35: 42000000 INFO @ Wed, 22 Jul 2020 15:54:35: 33000000 INFO @ Wed, 22 Jul 2020 15:54:40: 43000000 INFO @ Wed, 22 Jul 2020 15:54:40: 46000000 INFO @ Wed, 22 Jul 2020 15:54:40: 34000000 INFO @ Wed, 22 Jul 2020 15:54:45: 44000000 INFO @ Wed, 22 Jul 2020 15:54:46: 47000000 INFO @ Wed, 22 Jul 2020 15:54:46: 35000000 INFO @ Wed, 22 Jul 2020 15:54:51: 45000000 INFO @ Wed, 22 Jul 2020 15:54:51: 48000000 INFO @ Wed, 22 Jul 2020 15:54:52: 36000000 INFO @ Wed, 22 Jul 2020 15:54:56: 46000000 INFO @ Wed, 22 Jul 2020 15:54:57: 49000000 INFO @ Wed, 22 Jul 2020 15:54:58: 37000000 INFO @ Wed, 22 Jul 2020 15:55:01: 47000000 INFO @ Wed, 22 Jul 2020 15:55:02: 50000000 INFO @ Wed, 22 Jul 2020 15:55:04: 38000000 INFO @ Wed, 22 Jul 2020 15:55:07: 48000000 INFO @ Wed, 22 Jul 2020 15:55:08: 51000000 INFO @ Wed, 22 Jul 2020 15:55:09: 39000000 INFO @ Wed, 22 Jul 2020 15:55:12: 49000000 INFO @ Wed, 22 Jul 2020 15:55:13: 52000000 INFO @ Wed, 22 Jul 2020 15:55:15: 40000000 INFO @ Wed, 22 Jul 2020 15:55:17: 50000000 INFO @ Wed, 22 Jul 2020 15:55:19: 53000000 INFO @ Wed, 22 Jul 2020 15:55:21: 41000000 INFO @ Wed, 22 Jul 2020 15:55:22: 51000000 INFO @ Wed, 22 Jul 2020 15:55:24: 54000000 INFO @ Wed, 22 Jul 2020 15:55:26: 42000000 INFO @ Wed, 22 Jul 2020 15:55:28: 52000000 INFO @ Wed, 22 Jul 2020 15:55:30: 55000000 INFO @ Wed, 22 Jul 2020 15:55:32: 43000000 INFO @ Wed, 22 Jul 2020 15:55:33: 53000000 INFO @ Wed, 22 Jul 2020 15:55:35: 56000000 INFO @ Wed, 22 Jul 2020 15:55:38: 44000000 INFO @ Wed, 22 Jul 2020 15:55:38: 54000000 INFO @ Wed, 22 Jul 2020 15:55:41: 57000000 INFO @ Wed, 22 Jul 2020 15:55:43: 45000000 INFO @ Wed, 22 Jul 2020 15:55:44: 55000000 INFO @ Wed, 22 Jul 2020 15:55:46: 58000000 INFO @ Wed, 22 Jul 2020 15:55:49: 46000000 INFO @ Wed, 22 Jul 2020 15:55:49: 56000000 INFO @ Wed, 22 Jul 2020 15:55:52: 59000000 INFO @ Wed, 22 Jul 2020 15:55:54: 57000000 INFO @ Wed, 22 Jul 2020 15:55:54: 47000000 INFO @ Wed, 22 Jul 2020 15:55:55: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:55:55: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:55:55: #1 total tags in treatment: 28443743 INFO @ Wed, 22 Jul 2020 15:55:55: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:55:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:55:56: #1 tags after filtering in treatment: 14453582 INFO @ Wed, 22 Jul 2020 15:55:56: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 15:55:56: #1 finished! INFO @ Wed, 22 Jul 2020 15:55:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:55:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:55:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:55:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:55:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:56:00: 58000000 INFO @ Wed, 22 Jul 2020 15:56:00: 48000000 INFO @ Wed, 22 Jul 2020 15:56:05: 59000000 INFO @ Wed, 22 Jul 2020 15:56:05: 49000000 INFO @ Wed, 22 Jul 2020 15:56:08: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:56:08: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:56:08: #1 total tags in treatment: 28443743 INFO @ Wed, 22 Jul 2020 15:56:08: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:56:09: #1 tags after filtering in treatment: 14453582 INFO @ Wed, 22 Jul 2020 15:56:09: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 15:56:09: #1 finished! INFO @ Wed, 22 Jul 2020 15:56:09: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:56:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:56:09: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:56:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:56:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 15:56:11: 50000000 INFO @ Wed, 22 Jul 2020 15:56:16: 51000000 INFO @ Wed, 22 Jul 2020 15:56:21: 52000000 INFO @ Wed, 22 Jul 2020 15:56:27: 53000000 INFO @ Wed, 22 Jul 2020 15:56:32: 54000000 INFO @ Wed, 22 Jul 2020 15:56:37: 55000000 INFO @ Wed, 22 Jul 2020 15:56:42: 56000000 INFO @ Wed, 22 Jul 2020 15:56:48: 57000000 INFO @ Wed, 22 Jul 2020 15:56:53: 58000000 INFO @ Wed, 22 Jul 2020 15:56:58: 59000000 INFO @ Wed, 22 Jul 2020 15:57:01: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 15:57:01: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 15:57:01: #1 total tags in treatment: 28443743 INFO @ Wed, 22 Jul 2020 15:57:01: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 15:57:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 15:57:02: #1 tags after filtering in treatment: 14453582 INFO @ Wed, 22 Jul 2020 15:57:02: #1 Redundant rate of treatment: 0.49 INFO @ Wed, 22 Jul 2020 15:57:02: #1 finished! INFO @ Wed, 22 Jul 2020 15:57:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 15:57:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 15:57:02: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 15:57:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 15:57:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8639476/SRX8639476.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling