Job ID = 14522091 SRX = SRX8598378 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32532827 spots for SRR12070716/SRR12070716.sra Written 32532827 spots for SRR12070716/SRR12070716.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:41 32532827 reads; of these: 32532827 (100.00%) were unpaired; of these: 10876379 (33.43%) aligned 0 times 18056391 (55.50%) aligned exactly 1 time 3600057 (11.07%) aligned >1 times 66.57% overall alignment rate Time searching: 00:05:41 Overall time: 00:05:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9358128 / 21656448 = 0.4321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:59: 1000000 INFO @ Sat, 15 Jan 2022 22:20:04: 2000000 INFO @ Sat, 15 Jan 2022 22:20:10: 3000000 INFO @ Sat, 15 Jan 2022 22:20:15: 4000000 INFO @ Sat, 15 Jan 2022 22:20:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:20:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:20:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:20:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:20:25: 6000000 INFO @ Sat, 15 Jan 2022 22:20:29: 1000000 INFO @ Sat, 15 Jan 2022 22:20:31: 7000000 INFO @ Sat, 15 Jan 2022 22:20:35: 2000000 INFO @ Sat, 15 Jan 2022 22:20:36: 8000000 INFO @ Sat, 15 Jan 2022 22:20:40: 3000000 INFO @ Sat, 15 Jan 2022 22:20:41: 9000000 INFO @ Sat, 15 Jan 2022 22:20:46: 4000000 INFO @ Sat, 15 Jan 2022 22:20:47: 10000000 INFO @ Sat, 15 Jan 2022 22:20:51: 5000000 INFO @ Sat, 15 Jan 2022 22:20:52: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:20:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:20:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:20:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:20:57: 6000000 INFO @ Sat, 15 Jan 2022 22:20:57: 12000000 INFO @ Sat, 15 Jan 2022 22:20:59: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:20:59: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:20:59: #1 total tags in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:20:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:59: #1 tags after filtering in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:20:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:21:00: 1000000 INFO @ Sat, 15 Jan 2022 22:21:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:21:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:21:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:21:03: 7000000 INFO @ Sat, 15 Jan 2022 22:21:04: 2000000 INFO @ Sat, 15 Jan 2022 22:21:08: 8000000 INFO @ Sat, 15 Jan 2022 22:21:09: 3000000 INFO @ Sat, 15 Jan 2022 22:21:13: 9000000 INFO @ Sat, 15 Jan 2022 22:21:14: 4000000 INFO @ Sat, 15 Jan 2022 22:21:19: 5000000 INFO @ Sat, 15 Jan 2022 22:21:19: 10000000 INFO @ Sat, 15 Jan 2022 22:21:23: 6000000 INFO @ Sat, 15 Jan 2022 22:21:24: 11000000 INFO @ Sat, 15 Jan 2022 22:21:28: 7000000 INFO @ Sat, 15 Jan 2022 22:21:30: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:21:31: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:21:31: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:21:31: #1 total tags in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:21:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:21:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:21:31: #1 tags after filtering in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:21:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:21:31: #1 finished! INFO @ Sat, 15 Jan 2022 22:21:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:21:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:21:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:21:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:21:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:21:33: 8000000 INFO @ Sat, 15 Jan 2022 22:21:37: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:21:42: 10000000 INFO @ Sat, 15 Jan 2022 22:21:46: 11000000 INFO @ Sat, 15 Jan 2022 22:21:51: 12000000 INFO @ Sat, 15 Jan 2022 22:21:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:21:52: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:21:52: #1 total tags in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:21:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:21:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:21:53: #1 tags after filtering in treatment: 12298320 INFO @ Sat, 15 Jan 2022 22:21:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:21:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:21:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:21:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:21:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:21:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:21:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598378/SRX8598378.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling