Job ID = 14522089 SRX = SRX8598376 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11970560 spots for SRR12070714/SRR12070714.sra Written 11970560 spots for SRR12070714/SRR12070714.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:53 11970560 reads; of these: 11970560 (100.00%) were unpaired; of these: 4051822 (33.85%) aligned 0 times 6508080 (54.37%) aligned exactly 1 time 1410658 (11.78%) aligned >1 times 66.15% overall alignment rate Time searching: 00:01:53 Overall time: 00:01:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2055390 / 7918738 = 0.2596 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:12:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:12:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:12:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:13:08: 1000000 INFO @ Sat, 15 Jan 2022 22:13:16: 2000000 INFO @ Sat, 15 Jan 2022 22:13:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:13:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:13:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:13:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:13:31: 4000000 INFO @ Sat, 15 Jan 2022 22:13:39: 5000000 INFO @ Sat, 15 Jan 2022 22:13:40: 1000000 INFO @ Sat, 15 Jan 2022 22:13:46: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:13:46: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:13:46: #1 total tags in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:13:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:13:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:13:46: #1 tags after filtering in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:13:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:13:46: #1 finished! INFO @ Sat, 15 Jan 2022 22:13:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:13:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:13:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:13:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:13:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:13:51: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:13:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:13:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:13:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:14:01: 3000000 INFO @ Sat, 15 Jan 2022 22:14:08: 1000000 INFO @ Sat, 15 Jan 2022 22:14:13: 4000000 INFO @ Sat, 15 Jan 2022 22:14:17: 2000000 INFO @ Sat, 15 Jan 2022 22:14:24: 5000000 INFO @ Sat, 15 Jan 2022 22:14:26: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:14:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:14:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:14:33: #1 total tags in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:14:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:14:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:14:33: #1 tags after filtering in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:14:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:14:33: #1 finished! INFO @ Sat, 15 Jan 2022 22:14:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:14:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:14:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:14:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:14:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:14:35: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:14:44: 5000000 INFO @ Sat, 15 Jan 2022 22:14:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 22:14:52: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 22:14:52: #1 total tags in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:14:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:14:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:14:52: #1 tags after filtering in treatment: 5863348 INFO @ Sat, 15 Jan 2022 22:14:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:14:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:14:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:14:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:14:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:14:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:14:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8598376/SRX8598376.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling