Job ID = 14519713 SRX = SRX8586262 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16632799 spots for SRR12057931/SRR12057931.sra Written 16632799 spots for SRR12057931/SRR12057931.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:34 16632799 reads; of these: 16632799 (100.00%) were paired; of these: 1227772 (7.38%) aligned concordantly 0 times 12920461 (77.68%) aligned concordantly exactly 1 time 2484566 (14.94%) aligned concordantly >1 times ---- 1227772 pairs aligned concordantly 0 times; of these: 89954 (7.33%) aligned discordantly 1 time ---- 1137818 pairs aligned 0 times concordantly or discordantly; of these: 2275636 mates make up the pairs; of these: 1908636 (83.87%) aligned 0 times 232942 (10.24%) aligned exactly 1 time 134058 (5.89%) aligned >1 times 94.26% overall alignment rate Time searching: 00:07:34 Overall time: 00:07:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1648164 / 15478615 = 0.1065 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:45:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:45:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:45:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:45:45: 1000000 INFO @ Sat, 15 Jan 2022 17:45:49: 2000000 INFO @ Sat, 15 Jan 2022 17:45:52: 3000000 INFO @ Sat, 15 Jan 2022 17:45:56: 4000000 INFO @ Sat, 15 Jan 2022 17:46:00: 5000000 INFO @ Sat, 15 Jan 2022 17:46:04: 6000000 INFO @ Sat, 15 Jan 2022 17:46:08: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:46:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:46:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:46:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:46:12: 8000000 INFO @ Sat, 15 Jan 2022 17:46:15: 1000000 INFO @ Sat, 15 Jan 2022 17:46:16: 9000000 INFO @ Sat, 15 Jan 2022 17:46:19: 2000000 INFO @ Sat, 15 Jan 2022 17:46:20: 10000000 INFO @ Sat, 15 Jan 2022 17:46:23: 3000000 INFO @ Sat, 15 Jan 2022 17:46:24: 11000000 INFO @ Sat, 15 Jan 2022 17:46:27: 4000000 INFO @ Sat, 15 Jan 2022 17:46:28: 12000000 INFO @ Sat, 15 Jan 2022 17:46:31: 5000000 INFO @ Sat, 15 Jan 2022 17:46:32: 13000000 INFO @ Sat, 15 Jan 2022 17:46:35: 6000000 INFO @ Sat, 15 Jan 2022 17:46:36: 14000000 INFO @ Sat, 15 Jan 2022 17:46:39: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:46:40: 15000000 INFO @ Sat, 15 Jan 2022 17:46:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:46:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:46:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:46:43: 8000000 INFO @ Sat, 15 Jan 2022 17:46:44: 16000000 INFO @ Sat, 15 Jan 2022 17:46:45: 1000000 INFO @ Sat, 15 Jan 2022 17:46:47: 9000000 INFO @ Sat, 15 Jan 2022 17:46:48: 17000000 INFO @ Sat, 15 Jan 2022 17:46:50: 2000000 INFO @ Sat, 15 Jan 2022 17:46:51: 10000000 INFO @ Sat, 15 Jan 2022 17:46:52: 18000000 INFO @ Sat, 15 Jan 2022 17:46:55: 3000000 INFO @ Sat, 15 Jan 2022 17:46:55: 11000000 INFO @ Sat, 15 Jan 2022 17:46:56: 19000000 INFO @ Sat, 15 Jan 2022 17:46:59: 12000000 INFO @ Sat, 15 Jan 2022 17:47:00: 4000000 INFO @ Sat, 15 Jan 2022 17:47:00: 20000000 INFO @ Sat, 15 Jan 2022 17:47:03: 13000000 INFO @ Sat, 15 Jan 2022 17:47:04: 21000000 INFO @ Sat, 15 Jan 2022 17:47:04: 5000000 INFO @ Sat, 15 Jan 2022 17:47:07: 14000000 INFO @ Sat, 15 Jan 2022 17:47:08: 22000000 INFO @ Sat, 15 Jan 2022 17:47:09: 6000000 INFO @ Sat, 15 Jan 2022 17:47:12: 15000000 INFO @ Sat, 15 Jan 2022 17:47:13: 23000000 INFO @ Sat, 15 Jan 2022 17:47:14: 7000000 INFO @ Sat, 15 Jan 2022 17:47:16: 16000000 INFO @ Sat, 15 Jan 2022 17:47:17: 24000000 INFO @ Sat, 15 Jan 2022 17:47:19: 8000000 INFO @ Sat, 15 Jan 2022 17:47:20: 17000000 INFO @ Sat, 15 Jan 2022 17:47:21: 25000000 INFO @ Sat, 15 Jan 2022 17:47:24: 9000000 INFO @ Sat, 15 Jan 2022 17:47:24: 18000000 INFO @ Sat, 15 Jan 2022 17:47:25: 26000000 INFO @ Sat, 15 Jan 2022 17:47:28: 19000000 INFO @ Sat, 15 Jan 2022 17:47:29: 10000000 INFO @ Sat, 15 Jan 2022 17:47:29: 27000000 INFO @ Sat, 15 Jan 2022 17:47:32: 20000000 INFO @ Sat, 15 Jan 2022 17:47:33: 28000000 INFO @ Sat, 15 Jan 2022 17:47:33: 11000000 INFO @ Sat, 15 Jan 2022 17:47:33: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 17:47:33: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 17:47:33: #1 total tags in treatment: 13759438 INFO @ Sat, 15 Jan 2022 17:47:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:47:34: #1 tags after filtering in treatment: 7784380 INFO @ Sat, 15 Jan 2022 17:47:34: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:47:34: #1 finished! INFO @ Sat, 15 Jan 2022 17:47:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:47:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:47:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:47:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:47:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:47:36: 21000000 INFO @ Sat, 15 Jan 2022 17:47:38: 12000000 INFO @ Sat, 15 Jan 2022 17:47:40: 22000000 INFO @ Sat, 15 Jan 2022 17:47:43: 13000000 INFO @ Sat, 15 Jan 2022 17:47:44: 23000000 INFO @ Sat, 15 Jan 2022 17:47:48: 14000000 INFO @ Sat, 15 Jan 2022 17:47:48: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:47:52: 15000000 INFO @ Sat, 15 Jan 2022 17:47:52: 25000000 INFO @ Sat, 15 Jan 2022 17:47:57: 26000000 INFO @ Sat, 15 Jan 2022 17:47:57: 16000000 INFO @ Sat, 15 Jan 2022 17:48:01: 27000000 INFO @ Sat, 15 Jan 2022 17:48:02: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:48:05: 28000000 INFO @ Sat, 15 Jan 2022 17:48:05: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 17:48:05: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 17:48:05: #1 total tags in treatment: 13759438 INFO @ Sat, 15 Jan 2022 17:48:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:05: #1 tags after filtering in treatment: 7784380 INFO @ Sat, 15 Jan 2022 17:48:05: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:48:05: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:48:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:48:06: 18000000 INFO @ Sat, 15 Jan 2022 17:48:11: 19000000 INFO @ Sat, 15 Jan 2022 17:48:16: 20000000 INFO @ Sat, 15 Jan 2022 17:48:20: 21000000 INFO @ Sat, 15 Jan 2022 17:48:25: 22000000 INFO @ Sat, 15 Jan 2022 17:48:30: 23000000 INFO @ Sat, 15 Jan 2022 17:48:34: 24000000 INFO @ Sat, 15 Jan 2022 17:48:39: 25000000 INFO @ Sat, 15 Jan 2022 17:48:44: 26000000 INFO @ Sat, 15 Jan 2022 17:48:48: 27000000 INFO @ Sat, 15 Jan 2022 17:48:53: 28000000 INFO @ Sat, 15 Jan 2022 17:48:53: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 17:48:53: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 17:48:53: #1 total tags in treatment: 13759438 INFO @ Sat, 15 Jan 2022 17:48:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:54: #1 tags after filtering in treatment: 7784380 INFO @ Sat, 15 Jan 2022 17:48:54: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:48:54: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:48:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8586262/SRX8586262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling