Job ID = 14521532 SRX = SRX8499429 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 44490032 spots for SRR11955179/SRR11955179.sra Written 44490032 spots for SRR11955179/SRR11955179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:04 44490032 reads; of these: 44490032 (100.00%) were paired; of these: 2436471 (5.48%) aligned concordantly 0 times 35550769 (79.91%) aligned concordantly exactly 1 time 6502792 (14.62%) aligned concordantly >1 times ---- 2436471 pairs aligned concordantly 0 times; of these: 191080 (7.84%) aligned discordantly 1 time ---- 2245391 pairs aligned 0 times concordantly or discordantly; of these: 4490782 mates make up the pairs; of these: 4103123 (91.37%) aligned 0 times 220255 (4.90%) aligned exactly 1 time 167404 (3.73%) aligned >1 times 95.39% overall alignment rate Time searching: 00:58:04 Overall time: 00:58:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 14112588 / 42223177 = 0.3342 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:52:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:52:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:52:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:53:04: 1000000 INFO @ Sat, 15 Jan 2022 22:53:10: 2000000 INFO @ Sat, 15 Jan 2022 22:53:16: 3000000 INFO @ Sat, 15 Jan 2022 22:53:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:53:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:53:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:53:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:53:28: 5000000 INFO @ Sat, 15 Jan 2022 22:53:34: 1000000 INFO @ Sat, 15 Jan 2022 22:53:34: 6000000 INFO @ Sat, 15 Jan 2022 22:53:40: 2000000 INFO @ Sat, 15 Jan 2022 22:53:41: 7000000 INFO @ Sat, 15 Jan 2022 22:53:47: 3000000 INFO @ Sat, 15 Jan 2022 22:53:47: 8000000 INFO @ Sat, 15 Jan 2022 22:53:54: 4000000 INFO @ Sat, 15 Jan 2022 22:53:54: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:53:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:53:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:53:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:54:00: 5000000 INFO @ Sat, 15 Jan 2022 22:54:01: 10000000 INFO @ Sat, 15 Jan 2022 22:54:04: 1000000 INFO @ Sat, 15 Jan 2022 22:54:07: 6000000 INFO @ Sat, 15 Jan 2022 22:54:07: 11000000 INFO @ Sat, 15 Jan 2022 22:54:11: 2000000 INFO @ Sat, 15 Jan 2022 22:54:14: 7000000 INFO @ Sat, 15 Jan 2022 22:54:14: 12000000 INFO @ Sat, 15 Jan 2022 22:54:18: 3000000 INFO @ Sat, 15 Jan 2022 22:54:21: 8000000 INFO @ Sat, 15 Jan 2022 22:54:21: 13000000 INFO @ Sat, 15 Jan 2022 22:54:25: 4000000 INFO @ Sat, 15 Jan 2022 22:54:28: 9000000 INFO @ Sat, 15 Jan 2022 22:54:28: 14000000 INFO @ Sat, 15 Jan 2022 22:54:32: 5000000 INFO @ Sat, 15 Jan 2022 22:54:34: 15000000 INFO @ Sat, 15 Jan 2022 22:54:34: 10000000 INFO @ Sat, 15 Jan 2022 22:54:39: 6000000 INFO @ Sat, 15 Jan 2022 22:54:41: 16000000 INFO @ Sat, 15 Jan 2022 22:54:41: 11000000 INFO @ Sat, 15 Jan 2022 22:54:46: 7000000 INFO @ Sat, 15 Jan 2022 22:54:48: 17000000 INFO @ Sat, 15 Jan 2022 22:54:48: 12000000 INFO @ Sat, 15 Jan 2022 22:54:53: 8000000 INFO @ Sat, 15 Jan 2022 22:54:55: 18000000 INFO @ Sat, 15 Jan 2022 22:54:55: 13000000 INFO @ Sat, 15 Jan 2022 22:55:01: 9000000 INFO @ Sat, 15 Jan 2022 22:55:01: 19000000 INFO @ Sat, 15 Jan 2022 22:55:02: 14000000 INFO @ Sat, 15 Jan 2022 22:55:08: 10000000 INFO @ Sat, 15 Jan 2022 22:55:08: 20000000 INFO @ Sat, 15 Jan 2022 22:55:08: 15000000 INFO @ Sat, 15 Jan 2022 22:55:15: 11000000 INFO @ Sat, 15 Jan 2022 22:55:15: 21000000 INFO @ Sat, 15 Jan 2022 22:55:15: 16000000 INFO @ Sat, 15 Jan 2022 22:55:22: 12000000 INFO @ Sat, 15 Jan 2022 22:55:22: 22000000 INFO @ Sat, 15 Jan 2022 22:55:22: 17000000 INFO @ Sat, 15 Jan 2022 22:55:29: 23000000 INFO @ Sat, 15 Jan 2022 22:55:29: 13000000 INFO @ Sat, 15 Jan 2022 22:55:29: 18000000 INFO @ Sat, 15 Jan 2022 22:55:35: 24000000 INFO @ Sat, 15 Jan 2022 22:55:36: 14000000 INFO @ Sat, 15 Jan 2022 22:55:36: 19000000 INFO @ Sat, 15 Jan 2022 22:55:42: 25000000 INFO @ Sat, 15 Jan 2022 22:55:43: 20000000 INFO @ Sat, 15 Jan 2022 22:55:43: 15000000 INFO @ Sat, 15 Jan 2022 22:55:49: 26000000 INFO @ Sat, 15 Jan 2022 22:55:50: 21000000 INFO @ Sat, 15 Jan 2022 22:55:50: 16000000 INFO @ Sat, 15 Jan 2022 22:55:56: 27000000 INFO @ Sat, 15 Jan 2022 22:55:56: 22000000 INFO @ Sat, 15 Jan 2022 22:55:57: 17000000 INFO @ Sat, 15 Jan 2022 22:56:03: 28000000 INFO @ Sat, 15 Jan 2022 22:56:03: 23000000 INFO @ Sat, 15 Jan 2022 22:56:04: 18000000 INFO @ Sat, 15 Jan 2022 22:56:09: 29000000 INFO @ Sat, 15 Jan 2022 22:56:10: 24000000 INFO @ Sat, 15 Jan 2022 22:56:12: 19000000 INFO @ Sat, 15 Jan 2022 22:56:16: 30000000 INFO @ Sat, 15 Jan 2022 22:56:17: 25000000 INFO @ Sat, 15 Jan 2022 22:56:19: 20000000 INFO @ Sat, 15 Jan 2022 22:56:23: 31000000 INFO @ Sat, 15 Jan 2022 22:56:24: 26000000 INFO @ Sat, 15 Jan 2022 22:56:26: 21000000 INFO @ Sat, 15 Jan 2022 22:56:30: 32000000 INFO @ Sat, 15 Jan 2022 22:56:31: 27000000 INFO @ Sat, 15 Jan 2022 22:56:33: 22000000 INFO @ Sat, 15 Jan 2022 22:56:37: 33000000 INFO @ Sat, 15 Jan 2022 22:56:37: 28000000 INFO @ Sat, 15 Jan 2022 22:56:40: 23000000 INFO @ Sat, 15 Jan 2022 22:56:43: 34000000 INFO @ Sat, 15 Jan 2022 22:56:44: 29000000 INFO @ Sat, 15 Jan 2022 22:56:47: 24000000 INFO @ Sat, 15 Jan 2022 22:56:50: 35000000 INFO @ Sat, 15 Jan 2022 22:56:51: 30000000 INFO @ Sat, 15 Jan 2022 22:56:54: 25000000 INFO @ Sat, 15 Jan 2022 22:56:57: 36000000 INFO @ Sat, 15 Jan 2022 22:56:58: 31000000 INFO @ Sat, 15 Jan 2022 22:57:01: 26000000 INFO @ Sat, 15 Jan 2022 22:57:04: 37000000 INFO @ Sat, 15 Jan 2022 22:57:05: 32000000 INFO @ Sat, 15 Jan 2022 22:57:08: 27000000 INFO @ Sat, 15 Jan 2022 22:57:11: 38000000 INFO @ Sat, 15 Jan 2022 22:57:12: 33000000 INFO @ Sat, 15 Jan 2022 22:57:15: 28000000 INFO @ Sat, 15 Jan 2022 22:57:18: 39000000 INFO @ Sat, 15 Jan 2022 22:57:19: 34000000 INFO @ Sat, 15 Jan 2022 22:57:22: 29000000 INFO @ Sat, 15 Jan 2022 22:57:24: 40000000 INFO @ Sat, 15 Jan 2022 22:57:25: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:57:30: 30000000 INFO @ Sat, 15 Jan 2022 22:57:31: 41000000 INFO @ Sat, 15 Jan 2022 22:57:32: 36000000 INFO @ Sat, 15 Jan 2022 22:57:36: 31000000 INFO @ Sat, 15 Jan 2022 22:57:38: 42000000 INFO @ Sat, 15 Jan 2022 22:57:39: 37000000 INFO @ Sat, 15 Jan 2022 22:57:43: 32000000 INFO @ Sat, 15 Jan 2022 22:57:45: 43000000 INFO @ Sat, 15 Jan 2022 22:57:45: 38000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:57:51: 33000000 INFO @ Sat, 15 Jan 2022 22:57:52: 39000000 INFO @ Sat, 15 Jan 2022 22:57:52: 44000000 INFO @ Sat, 15 Jan 2022 22:57:58: 34000000 INFO @ Sat, 15 Jan 2022 22:57:59: 40000000 INFO @ Sat, 15 Jan 2022 22:57:59: 45000000 INFO @ Sat, 15 Jan 2022 22:58:05: 35000000 INFO @ Sat, 15 Jan 2022 22:58:06: 41000000 INFO @ Sat, 15 Jan 2022 22:58:06: 46000000 INFO @ Sat, 15 Jan 2022 22:58:12: 36000000 INFO @ Sat, 15 Jan 2022 22:58:13: 47000000 INFO @ Sat, 15 Jan 2022 22:58:13: 42000000 INFO @ Sat, 15 Jan 2022 22:58:19: 37000000 INFO @ Sat, 15 Jan 2022 22:58:20: 48000000 INFO @ Sat, 15 Jan 2022 22:58:20: 43000000 INFO @ Sat, 15 Jan 2022 22:58:26: 38000000 INFO @ Sat, 15 Jan 2022 22:58:27: 49000000 INFO @ Sat, 15 Jan 2022 22:58:27: 44000000 INFO @ Sat, 15 Jan 2022 22:58:33: 39000000 INFO @ Sat, 15 Jan 2022 22:58:34: 50000000 INFO @ Sat, 15 Jan 2022 22:58:34: 45000000 INFO @ Sat, 15 Jan 2022 22:58:40: 40000000 INFO @ Sat, 15 Jan 2022 22:58:41: 51000000 INFO @ Sat, 15 Jan 2022 22:58:41: 46000000 INFO @ Sat, 15 Jan 2022 22:58:47: 41000000 INFO @ Sat, 15 Jan 2022 22:58:48: 52000000 INFO @ Sat, 15 Jan 2022 22:58:48: 47000000 INFO @ Sat, 15 Jan 2022 22:58:54: 42000000 INFO @ Sat, 15 Jan 2022 22:58:55: 53000000 INFO @ Sat, 15 Jan 2022 22:58:55: 48000000 INFO @ Sat, 15 Jan 2022 22:59:01: 43000000 INFO @ Sat, 15 Jan 2022 22:59:02: 54000000 INFO @ Sat, 15 Jan 2022 22:59:02: 49000000 INFO @ Sat, 15 Jan 2022 22:59:08: 44000000 INFO @ Sat, 15 Jan 2022 22:59:09: 55000000 INFO @ Sat, 15 Jan 2022 22:59:09: 50000000 INFO @ Sat, 15 Jan 2022 22:59:15: 45000000 INFO @ Sat, 15 Jan 2022 22:59:16: 56000000 INFO @ Sat, 15 Jan 2022 22:59:16: 51000000 INFO @ Sat, 15 Jan 2022 22:59:21: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:59:21: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:59:21: #1 total tags in treatment: 27991106 INFO @ Sat, 15 Jan 2022 22:59:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:59:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:59:21: #1 tags after filtering in treatment: 15400635 INFO @ Sat, 15 Jan 2022 22:59:21: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 22:59:21: #1 finished! INFO @ Sat, 15 Jan 2022 22:59:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:59:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:59:22: 46000000 INFO @ Sat, 15 Jan 2022 22:59:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:59:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:59:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:59:23: 52000000 INFO @ Sat, 15 Jan 2022 22:59:29: 47000000 INFO @ Sat, 15 Jan 2022 22:59:29: 53000000 INFO @ Sat, 15 Jan 2022 22:59:35: 54000000 INFO @ Sat, 15 Jan 2022 22:59:36: 48000000 INFO @ Sat, 15 Jan 2022 22:59:41: 55000000 INFO @ Sat, 15 Jan 2022 22:59:43: 49000000 INFO @ Sat, 15 Jan 2022 22:59:48: 56000000 INFO @ Sat, 15 Jan 2022 22:59:50: 50000000 INFO @ Sat, 15 Jan 2022 22:59:52: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:59:52: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:59:52: #1 total tags in treatment: 27991106 INFO @ Sat, 15 Jan 2022 22:59:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:59:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:59:53: #1 tags after filtering in treatment: 15400635 INFO @ Sat, 15 Jan 2022 22:59:53: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 22:59:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:59:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:59:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:59:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:59:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:59:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:59:57: 51000000 INFO @ Sat, 15 Jan 2022 23:00:03: 52000000 INFO @ Sat, 15 Jan 2022 23:00:10: 53000000 INFO @ Sat, 15 Jan 2022 23:00:16: 54000000 INFO @ Sat, 15 Jan 2022 23:00:23: 55000000 INFO @ Sat, 15 Jan 2022 23:00:29: 56000000 INFO @ Sat, 15 Jan 2022 23:00:34: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 23:00:34: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 23:00:34: #1 total tags in treatment: 27991106 INFO @ Sat, 15 Jan 2022 23:00:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:00:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:00:34: #1 tags after filtering in treatment: 15400635 INFO @ Sat, 15 Jan 2022 23:00:34: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 23:00:34: #1 finished! INFO @ Sat, 15 Jan 2022 23:00:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:00:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:00:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:00:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:00:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499429/SRX8499429.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling