Job ID = 14521531 SRX = SRX8499428 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17857481 spots for SRR11955178/SRR11955178.sra Written 17857481 spots for SRR11955178/SRR11955178.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:55 17857481 reads; of these: 17857481 (100.00%) were paired; of these: 1315708 (7.37%) aligned concordantly 0 times 14111397 (79.02%) aligned concordantly exactly 1 time 2430376 (13.61%) aligned concordantly >1 times ---- 1315708 pairs aligned concordantly 0 times; of these: 39269 (2.98%) aligned discordantly 1 time ---- 1276439 pairs aligned 0 times concordantly or discordantly; of these: 2552878 mates make up the pairs; of these: 2410174 (94.41%) aligned 0 times 94167 (3.69%) aligned exactly 1 time 48537 (1.90%) aligned >1 times 93.25% overall alignment rate Time searching: 00:33:55 Overall time: 00:33:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3876482 / 16576928 = 0.2338 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:56: 1000000 INFO @ Sat, 15 Jan 2022 21:52:01: 2000000 INFO @ Sat, 15 Jan 2022 21:52:06: 3000000 INFO @ Sat, 15 Jan 2022 21:52:11: 4000000 INFO @ Sat, 15 Jan 2022 21:52:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:21: 6000000 INFO @ Sat, 15 Jan 2022 21:52:27: 7000000 INFO @ Sat, 15 Jan 2022 21:52:28: 1000000 INFO @ Sat, 15 Jan 2022 21:52:32: 8000000 INFO @ Sat, 15 Jan 2022 21:52:34: 2000000 INFO @ Sat, 15 Jan 2022 21:52:38: 9000000 INFO @ Sat, 15 Jan 2022 21:52:41: 3000000 INFO @ Sat, 15 Jan 2022 21:52:44: 10000000 INFO @ Sat, 15 Jan 2022 21:52:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:52:49: 11000000 INFO @ Sat, 15 Jan 2022 21:52:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:52:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:52:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:52:55: 12000000 INFO @ Sat, 15 Jan 2022 21:52:55: 5000000 INFO @ Sat, 15 Jan 2022 21:52:58: 1000000 INFO @ Sat, 15 Jan 2022 21:53:00: 13000000 INFO @ Sat, 15 Jan 2022 21:53:02: 6000000 INFO @ Sat, 15 Jan 2022 21:53:04: 2000000 INFO @ Sat, 15 Jan 2022 21:53:06: 14000000 INFO @ Sat, 15 Jan 2022 21:53:09: 7000000 INFO @ Sat, 15 Jan 2022 21:53:11: 3000000 INFO @ Sat, 15 Jan 2022 21:53:12: 15000000 INFO @ Sat, 15 Jan 2022 21:53:16: 8000000 INFO @ Sat, 15 Jan 2022 21:53:17: 16000000 INFO @ Sat, 15 Jan 2022 21:53:18: 4000000 INFO @ Sat, 15 Jan 2022 21:53:23: 17000000 INFO @ Sat, 15 Jan 2022 21:53:23: 9000000 INFO @ Sat, 15 Jan 2022 21:53:25: 5000000 INFO @ Sat, 15 Jan 2022 21:53:28: 18000000 INFO @ Sat, 15 Jan 2022 21:53:30: 10000000 INFO @ Sat, 15 Jan 2022 21:53:32: 6000000 INFO @ Sat, 15 Jan 2022 21:53:34: 19000000 INFO @ Sat, 15 Jan 2022 21:53:37: 11000000 INFO @ Sat, 15 Jan 2022 21:53:39: 7000000 INFO @ Sat, 15 Jan 2022 21:53:40: 20000000 INFO @ Sat, 15 Jan 2022 21:53:44: 12000000 INFO @ Sat, 15 Jan 2022 21:53:45: 8000000 INFO @ Sat, 15 Jan 2022 21:53:45: 21000000 INFO @ Sat, 15 Jan 2022 21:53:51: 13000000 INFO @ Sat, 15 Jan 2022 21:53:51: 22000000 INFO @ Sat, 15 Jan 2022 21:53:52: 9000000 INFO @ Sat, 15 Jan 2022 21:53:57: 23000000 INFO @ Sat, 15 Jan 2022 21:53:58: 14000000 INFO @ Sat, 15 Jan 2022 21:53:59: 10000000 INFO @ Sat, 15 Jan 2022 21:54:02: 24000000 INFO @ Sat, 15 Jan 2022 21:54:05: 15000000 INFO @ Sat, 15 Jan 2022 21:54:06: 11000000 INFO @ Sat, 15 Jan 2022 21:54:08: 25000000 INFO @ Sat, 15 Jan 2022 21:54:11: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:54:11: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:54:11: #1 total tags in treatment: 12672820 INFO @ Sat, 15 Jan 2022 21:54:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:11: #1 tags after filtering in treatment: 9038152 INFO @ Sat, 15 Jan 2022 21:54:11: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:54:11: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:12: 16000000 INFO @ Sat, 15 Jan 2022 21:54:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:13: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:54:18: 17000000 INFO @ Sat, 15 Jan 2022 21:54:20: 13000000 INFO @ Sat, 15 Jan 2022 21:54:25: 18000000 INFO @ Sat, 15 Jan 2022 21:54:26: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:54:32: 19000000 INFO @ Sat, 15 Jan 2022 21:54:33: 15000000 INFO @ Sat, 15 Jan 2022 21:54:39: 20000000 INFO @ Sat, 15 Jan 2022 21:54:40: 16000000 INFO @ Sat, 15 Jan 2022 21:54:46: 17000000 INFO @ Sat, 15 Jan 2022 21:54:46: 21000000 INFO @ Sat, 15 Jan 2022 21:54:53: 18000000 INFO @ Sat, 15 Jan 2022 21:54:53: 22000000 INFO @ Sat, 15 Jan 2022 21:55:00: 19000000 INFO @ Sat, 15 Jan 2022 21:55:00: 23000000 INFO @ Sat, 15 Jan 2022 21:55:07: 24000000 INFO @ Sat, 15 Jan 2022 21:55:07: 20000000 INFO @ Sat, 15 Jan 2022 21:55:14: 25000000 INFO @ Sat, 15 Jan 2022 21:55:14: 21000000 INFO @ Sat, 15 Jan 2022 21:55:18: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:55:18: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:55:18: #1 total tags in treatment: 12672820 INFO @ Sat, 15 Jan 2022 21:55:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:55:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:55:18: #1 tags after filtering in treatment: 9038152 INFO @ Sat, 15 Jan 2022 21:55:18: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:55:18: #1 finished! INFO @ Sat, 15 Jan 2022 21:55:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:55:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:55:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:55:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:55:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:55:21: 22000000 INFO @ Sat, 15 Jan 2022 21:55:28: 23000000 INFO @ Sat, 15 Jan 2022 21:55:34: 24000000 INFO @ Sat, 15 Jan 2022 21:55:41: 25000000 INFO @ Sat, 15 Jan 2022 21:55:45: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:55:45: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:55:45: #1 total tags in treatment: 12672820 INFO @ Sat, 15 Jan 2022 21:55:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:55:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:55:45: #1 tags after filtering in treatment: 9038152 INFO @ Sat, 15 Jan 2022 21:55:45: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:55:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:55:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:55:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:55:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:55:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:55:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499428/SRX8499428.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling