Job ID = 14521530 SRX = SRX8499427 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22477413 spots for SRR11955177/SRR11955177.sra Written 22477413 spots for SRR11955177/SRR11955177.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:36:05 22477413 reads; of these: 22477413 (100.00%) were paired; of these: 1569811 (6.98%) aligned concordantly 0 times 17374320 (77.30%) aligned concordantly exactly 1 time 3533282 (15.72%) aligned concordantly >1 times ---- 1569811 pairs aligned concordantly 0 times; of these: 62080 (3.95%) aligned discordantly 1 time ---- 1507731 pairs aligned 0 times concordantly or discordantly; of these: 3015462 mates make up the pairs; of these: 2840904 (94.21%) aligned 0 times 102486 (3.40%) aligned exactly 1 time 72072 (2.39%) aligned >1 times 93.68% overall alignment rate Time searching: 00:36:05 Overall time: 00:36:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6012153 / 20963755 = 0.2868 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:56:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:56:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:56:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:56:22: 1000000 INFO @ Sat, 15 Jan 2022 21:56:28: 2000000 INFO @ Sat, 15 Jan 2022 21:56:34: 3000000 INFO @ Sat, 15 Jan 2022 21:56:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:56:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:56:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:56:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:56:46: 5000000 INFO @ Sat, 15 Jan 2022 21:56:53: 1000000 INFO @ Sat, 15 Jan 2022 21:56:54: 6000000 INFO @ Sat, 15 Jan 2022 21:57:00: 2000000 INFO @ Sat, 15 Jan 2022 21:57:01: 7000000 INFO @ Sat, 15 Jan 2022 21:57:07: 3000000 INFO @ Sat, 15 Jan 2022 21:57:08: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:57:15: 4000000 INFO @ Sat, 15 Jan 2022 21:57:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:57:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:57:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:57:16: 9000000 INFO @ Sat, 15 Jan 2022 21:57:22: 5000000 INFO @ Sat, 15 Jan 2022 21:57:23: 1000000 INFO @ Sat, 15 Jan 2022 21:57:23: 10000000 INFO @ Sat, 15 Jan 2022 21:57:30: 6000000 INFO @ Sat, 15 Jan 2022 21:57:30: 2000000 INFO @ Sat, 15 Jan 2022 21:57:31: 11000000 INFO @ Sat, 15 Jan 2022 21:57:38: 7000000 INFO @ Sat, 15 Jan 2022 21:57:38: 3000000 INFO @ Sat, 15 Jan 2022 21:57:39: 12000000 INFO @ Sat, 15 Jan 2022 21:57:46: 8000000 INFO @ Sat, 15 Jan 2022 21:57:47: 4000000 INFO @ Sat, 15 Jan 2022 21:57:47: 13000000 INFO @ Sat, 15 Jan 2022 21:57:55: 9000000 INFO @ Sat, 15 Jan 2022 21:57:55: 5000000 INFO @ Sat, 15 Jan 2022 21:57:55: 14000000 INFO @ Sat, 15 Jan 2022 21:58:03: 10000000 INFO @ Sat, 15 Jan 2022 21:58:03: 15000000 INFO @ Sat, 15 Jan 2022 21:58:03: 6000000 INFO @ Sat, 15 Jan 2022 21:58:11: 11000000 INFO @ Sat, 15 Jan 2022 21:58:11: 16000000 INFO @ Sat, 15 Jan 2022 21:58:11: 7000000 INFO @ Sat, 15 Jan 2022 21:58:19: 17000000 INFO @ Sat, 15 Jan 2022 21:58:19: 8000000 INFO @ Sat, 15 Jan 2022 21:58:19: 12000000 INFO @ Sat, 15 Jan 2022 21:58:26: 18000000 INFO @ Sat, 15 Jan 2022 21:58:27: 9000000 INFO @ Sat, 15 Jan 2022 21:58:27: 13000000 INFO @ Sat, 15 Jan 2022 21:58:34: 19000000 INFO @ Sat, 15 Jan 2022 21:58:34: 10000000 INFO @ Sat, 15 Jan 2022 21:58:34: 14000000 INFO @ Sat, 15 Jan 2022 21:58:42: 20000000 INFO @ Sat, 15 Jan 2022 21:58:42: 11000000 INFO @ Sat, 15 Jan 2022 21:58:42: 15000000 INFO @ Sat, 15 Jan 2022 21:58:50: 21000000 INFO @ Sat, 15 Jan 2022 21:58:50: 12000000 INFO @ Sat, 15 Jan 2022 21:58:50: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:58:57: 22000000 INFO @ Sat, 15 Jan 2022 21:58:58: 13000000 INFO @ Sat, 15 Jan 2022 21:58:58: 17000000 INFO @ Sat, 15 Jan 2022 21:59:05: 23000000 INFO @ Sat, 15 Jan 2022 21:59:06: 14000000 INFO @ Sat, 15 Jan 2022 21:59:06: 18000000 INFO @ Sat, 15 Jan 2022 21:59:13: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:59:13: 15000000 INFO @ Sat, 15 Jan 2022 21:59:14: 19000000 INFO @ Sat, 15 Jan 2022 21:59:20: 25000000 INFO @ Sat, 15 Jan 2022 21:59:21: 16000000 INFO @ Sat, 15 Jan 2022 21:59:22: 20000000 INFO @ Sat, 15 Jan 2022 21:59:28: 26000000 INFO @ Sat, 15 Jan 2022 21:59:29: 17000000 INFO @ Sat, 15 Jan 2022 21:59:29: 21000000 INFO @ Sat, 15 Jan 2022 21:59:36: 27000000 INFO @ Sat, 15 Jan 2022 21:59:37: 18000000 INFO @ Sat, 15 Jan 2022 21:59:37: 22000000 INFO @ Sat, 15 Jan 2022 21:59:43: 28000000 INFO @ Sat, 15 Jan 2022 21:59:44: 19000000 INFO @ Sat, 15 Jan 2022 21:59:45: 23000000 INFO @ Sat, 15 Jan 2022 21:59:51: 29000000 INFO @ Sat, 15 Jan 2022 21:59:52: 20000000 INFO @ Sat, 15 Jan 2022 21:59:53: 24000000 INFO @ Sat, 15 Jan 2022 21:59:58: 30000000 INFO @ Sat, 15 Jan 2022 21:59:59: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:59:59: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:59:59: #1 total tags in treatment: 14909986 INFO @ Sat, 15 Jan 2022 21:59:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:59:59: #1 tags after filtering in treatment: 10112157 INFO @ Sat, 15 Jan 2022 21:59:59: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 21:59:59: #1 finished! INFO @ Sat, 15 Jan 2022 21:59:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:59:59: 21000000 INFO @ Sat, 15 Jan 2022 22:00:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:00:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:00:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:00:01: 25000000 INFO @ Sat, 15 Jan 2022 22:00:07: 22000000 INFO @ Sat, 15 Jan 2022 22:00:08: 26000000 INFO @ Sat, 15 Jan 2022 22:00:15: 23000000 INFO @ Sat, 15 Jan 2022 22:00:16: 27000000 INFO @ Sat, 15 Jan 2022 22:00:22: 24000000 INFO @ Sat, 15 Jan 2022 22:00:24: 28000000 INFO @ Sat, 15 Jan 2022 22:00:30: 25000000 INFO @ Sat, 15 Jan 2022 22:00:32: 29000000 INFO @ Sat, 15 Jan 2022 22:00:37: 26000000 INFO @ Sat, 15 Jan 2022 22:00:40: 30000000 INFO @ Sat, 15 Jan 2022 22:00:40: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:00:40: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:00:40: #1 total tags in treatment: 14909986 INFO @ Sat, 15 Jan 2022 22:00:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:00:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:00:40: #1 tags after filtering in treatment: 10112157 INFO @ Sat, 15 Jan 2022 22:00:40: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:00:40: #1 finished! INFO @ Sat, 15 Jan 2022 22:00:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:00:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:00:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:00:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:00:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:00:44: 27000000 INFO @ Sat, 15 Jan 2022 22:00:51: 28000000 INFO @ Sat, 15 Jan 2022 22:00:57: 29000000 INFO @ Sat, 15 Jan 2022 22:01:03: 30000000 INFO @ Sat, 15 Jan 2022 22:01:04: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 22:01:04: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 22:01:04: #1 total tags in treatment: 14909986 INFO @ Sat, 15 Jan 2022 22:01:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:04: #1 tags after filtering in treatment: 10112157 INFO @ Sat, 15 Jan 2022 22:01:04: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:01:04: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499427/SRX8499427.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling