Job ID = 14521529 SRX = SRX8499426 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25376700 spots for SRR11955176/SRR11955176.sra Written 25376700 spots for SRR11955176/SRR11955176.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:12 25376700 reads; of these: 25376700 (100.00%) were paired; of these: 1631637 (6.43%) aligned concordantly 0 times 19685705 (77.57%) aligned concordantly exactly 1 time 4059358 (16.00%) aligned concordantly >1 times ---- 1631637 pairs aligned concordantly 0 times; of these: 81473 (4.99%) aligned discordantly 1 time ---- 1550164 pairs aligned 0 times concordantly or discordantly; of these: 3100328 mates make up the pairs; of these: 2870385 (92.58%) aligned 0 times 133905 (4.32%) aligned exactly 1 time 96038 (3.10%) aligned >1 times 94.34% overall alignment rate Time searching: 00:27:12 Overall time: 00:27:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 11683437 / 23816985 = 0.4906 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:58: 1000000 INFO @ Sat, 15 Jan 2022 21:48:03: 2000000 INFO @ Sat, 15 Jan 2022 21:48:08: 3000000 INFO @ Sat, 15 Jan 2022 21:48:13: 4000000 INFO @ Sat, 15 Jan 2022 21:48:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:23: 6000000 INFO @ Sat, 15 Jan 2022 21:48:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:28: 7000000 INFO @ Sat, 15 Jan 2022 21:48:29: 1000000 INFO @ Sat, 15 Jan 2022 21:48:34: 8000000 INFO @ Sat, 15 Jan 2022 21:48:34: 2000000 INFO @ Sat, 15 Jan 2022 21:48:39: 9000000 INFO @ Sat, 15 Jan 2022 21:48:39: 3000000 INFO @ Sat, 15 Jan 2022 21:48:44: 10000000 INFO @ Sat, 15 Jan 2022 21:48:45: 4000000 INFO @ Sat, 15 Jan 2022 21:48:50: 11000000 INFO @ Sat, 15 Jan 2022 21:48:50: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:55: 12000000 INFO @ Sat, 15 Jan 2022 21:48:55: 6000000 INFO @ Sat, 15 Jan 2022 21:48:59: 1000000 INFO @ Sat, 15 Jan 2022 21:49:00: 13000000 INFO @ Sat, 15 Jan 2022 21:49:01: 7000000 INFO @ Sat, 15 Jan 2022 21:49:04: 2000000 INFO @ Sat, 15 Jan 2022 21:49:06: 14000000 INFO @ Sat, 15 Jan 2022 21:49:06: 8000000 INFO @ Sat, 15 Jan 2022 21:49:09: 3000000 INFO @ Sat, 15 Jan 2022 21:49:11: 15000000 INFO @ Sat, 15 Jan 2022 21:49:11: 9000000 INFO @ Sat, 15 Jan 2022 21:49:15: 4000000 INFO @ Sat, 15 Jan 2022 21:49:16: 16000000 INFO @ Sat, 15 Jan 2022 21:49:17: 10000000 INFO @ Sat, 15 Jan 2022 21:49:20: 5000000 INFO @ Sat, 15 Jan 2022 21:49:22: 17000000 INFO @ Sat, 15 Jan 2022 21:49:22: 11000000 INFO @ Sat, 15 Jan 2022 21:49:25: 6000000 INFO @ Sat, 15 Jan 2022 21:49:27: 18000000 INFO @ Sat, 15 Jan 2022 21:49:27: 12000000 INFO @ Sat, 15 Jan 2022 21:49:30: 7000000 INFO @ Sat, 15 Jan 2022 21:49:33: 19000000 INFO @ Sat, 15 Jan 2022 21:49:33: 13000000 INFO @ Sat, 15 Jan 2022 21:49:36: 8000000 INFO @ Sat, 15 Jan 2022 21:49:38: 20000000 INFO @ Sat, 15 Jan 2022 21:49:38: 14000000 INFO @ Sat, 15 Jan 2022 21:49:41: 9000000 INFO @ Sat, 15 Jan 2022 21:49:43: 21000000 INFO @ Sat, 15 Jan 2022 21:49:44: 15000000 INFO @ Sat, 15 Jan 2022 21:49:46: 10000000 INFO @ Sat, 15 Jan 2022 21:49:49: 22000000 INFO @ Sat, 15 Jan 2022 21:49:49: 16000000 INFO @ Sat, 15 Jan 2022 21:49:52: 11000000 INFO @ Sat, 15 Jan 2022 21:49:54: 23000000 INFO @ Sat, 15 Jan 2022 21:49:54: 17000000 INFO @ Sat, 15 Jan 2022 21:49:57: 12000000 INFO @ Sat, 15 Jan 2022 21:49:59: 24000000 INFO @ Sat, 15 Jan 2022 21:50:00: 18000000 INFO @ Sat, 15 Jan 2022 21:50:02: 13000000 INFO @ Sat, 15 Jan 2022 21:50:02: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:50:02: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:50:02: #1 total tags in treatment: 12094176 INFO @ Sat, 15 Jan 2022 21:50:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:50:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:50:02: #1 tags after filtering in treatment: 8451092 INFO @ Sat, 15 Jan 2022 21:50:02: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:50:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:50:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:50:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:50:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:50:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:50:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:50:05: 19000000 INFO @ Sat, 15 Jan 2022 21:50:07: 14000000 INFO @ Sat, 15 Jan 2022 21:50:10: 20000000 INFO @ Sat, 15 Jan 2022 21:50:13: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:50:16: 21000000 INFO @ Sat, 15 Jan 2022 21:50:18: 16000000 INFO @ Sat, 15 Jan 2022 21:50:21: 22000000 INFO @ Sat, 15 Jan 2022 21:50:23: 17000000 INFO @ Sat, 15 Jan 2022 21:50:26: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:50:28: 18000000 INFO @ Sat, 15 Jan 2022 21:50:31: 24000000 INFO @ Sat, 15 Jan 2022 21:50:34: 19000000 INFO @ Sat, 15 Jan 2022 21:50:34: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:50:34: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:50:34: #1 total tags in treatment: 12094176 INFO @ Sat, 15 Jan 2022 21:50:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:50:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:50:34: #1 tags after filtering in treatment: 8451092 INFO @ Sat, 15 Jan 2022 21:50:34: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:50:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:50:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:50:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:50:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:50:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:50:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:50:39: 20000000 INFO @ Sat, 15 Jan 2022 21:50:44: 21000000 INFO @ Sat, 15 Jan 2022 21:50:49: 22000000 INFO @ Sat, 15 Jan 2022 21:50:54: 23000000 INFO @ Sat, 15 Jan 2022 21:50:59: 24000000 INFO @ Sat, 15 Jan 2022 21:51:02: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:51:02: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:51:02: #1 total tags in treatment: 12094176 INFO @ Sat, 15 Jan 2022 21:51:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:51:02: #1 tags after filtering in treatment: 8451092 INFO @ Sat, 15 Jan 2022 21:51:02: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:51:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:51:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:51:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:51:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:51:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499426/SRX8499426.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling