Job ID = 14521528 SRX = SRX8499425 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18105193 spots for SRR11955175/SRR11955175.sra Written 18105193 spots for SRR11955175/SRR11955175.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:24 18105193 reads; of these: 18105193 (100.00%) were paired; of these: 6387670 (35.28%) aligned concordantly 0 times 9969155 (55.06%) aligned concordantly exactly 1 time 1748368 (9.66%) aligned concordantly >1 times ---- 6387670 pairs aligned concordantly 0 times; of these: 2434583 (38.11%) aligned discordantly 1 time ---- 3953087 pairs aligned 0 times concordantly or discordantly; of these: 7906174 mates make up the pairs; of these: 6635244 (83.92%) aligned 0 times 381520 (4.83%) aligned exactly 1 time 889410 (11.25%) aligned >1 times 81.68% overall alignment rate Time searching: 00:14:24 Overall time: 00:14:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1919118 / 13996470 = 0.1371 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:56: 1000000 INFO @ Sat, 15 Jan 2022 21:31:01: 2000000 INFO @ Sat, 15 Jan 2022 21:31:06: 3000000 INFO @ Sat, 15 Jan 2022 21:31:12: 4000000 INFO @ Sat, 15 Jan 2022 21:31:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:31:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:31:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:31:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:31:23: 6000000 INFO @ Sat, 15 Jan 2022 21:31:26: 1000000 INFO @ Sat, 15 Jan 2022 21:31:29: 7000000 INFO @ Sat, 15 Jan 2022 21:31:32: 2000000 INFO @ Sat, 15 Jan 2022 21:31:35: 8000000 INFO @ Sat, 15 Jan 2022 21:31:38: 3000000 INFO @ Sat, 15 Jan 2022 21:31:41: 9000000 INFO @ Sat, 15 Jan 2022 21:31:44: 4000000 INFO @ Sat, 15 Jan 2022 21:31:47: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:31:50: 5000000 INFO @ Sat, 15 Jan 2022 21:31:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:31:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:31:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:31:53: 11000000 INFO @ Sat, 15 Jan 2022 21:31:56: 6000000 INFO @ Sat, 15 Jan 2022 21:31:59: 1000000 INFO @ Sat, 15 Jan 2022 21:32:00: 12000000 INFO @ Sat, 15 Jan 2022 21:32:03: 7000000 INFO @ Sat, 15 Jan 2022 21:32:06: 13000000 INFO @ Sat, 15 Jan 2022 21:32:07: 2000000 INFO @ Sat, 15 Jan 2022 21:32:09: 8000000 INFO @ Sat, 15 Jan 2022 21:32:13: 14000000 INFO @ Sat, 15 Jan 2022 21:32:15: 3000000 INFO @ Sat, 15 Jan 2022 21:32:15: 9000000 INFO @ Sat, 15 Jan 2022 21:32:19: 15000000 INFO @ Sat, 15 Jan 2022 21:32:22: 10000000 INFO @ Sat, 15 Jan 2022 21:32:22: 4000000 INFO @ Sat, 15 Jan 2022 21:32:25: 16000000 INFO @ Sat, 15 Jan 2022 21:32:28: 11000000 INFO @ Sat, 15 Jan 2022 21:32:30: 5000000 INFO @ Sat, 15 Jan 2022 21:32:32: 17000000 INFO @ Sat, 15 Jan 2022 21:32:35: 12000000 INFO @ Sat, 15 Jan 2022 21:32:37: 6000000 INFO @ Sat, 15 Jan 2022 21:32:38: 18000000 INFO @ Sat, 15 Jan 2022 21:32:41: 13000000 INFO @ Sat, 15 Jan 2022 21:32:45: 19000000 INFO @ Sat, 15 Jan 2022 21:32:45: 7000000 INFO @ Sat, 15 Jan 2022 21:32:47: 14000000 INFO @ Sat, 15 Jan 2022 21:32:51: 20000000 INFO @ Sat, 15 Jan 2022 21:32:53: 8000000 INFO @ Sat, 15 Jan 2022 21:32:54: 15000000 INFO @ Sat, 15 Jan 2022 21:32:57: 21000000 INFO @ Sat, 15 Jan 2022 21:33:00: 16000000 INFO @ Sat, 15 Jan 2022 21:33:00: 9000000 INFO @ Sat, 15 Jan 2022 21:33:04: 22000000 INFO @ Sat, 15 Jan 2022 21:33:06: 17000000 INFO @ Sat, 15 Jan 2022 21:33:08: 10000000 INFO @ Sat, 15 Jan 2022 21:33:10: 23000000 INFO @ Sat, 15 Jan 2022 21:33:12: 18000000 INFO @ Sat, 15 Jan 2022 21:33:16: 11000000 INFO @ Sat, 15 Jan 2022 21:33:17: 24000000 INFO @ Sat, 15 Jan 2022 21:33:19: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:33:23: 25000000 INFO @ Sat, 15 Jan 2022 21:33:23: 12000000 INFO @ Sat, 15 Jan 2022 21:33:25: 20000000 INFO @ Sat, 15 Jan 2022 21:33:28: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:33:28: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:33:28: #1 total tags in treatment: 10110051 INFO @ Sat, 15 Jan 2022 21:33:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:33:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:33:28: #1 tags after filtering in treatment: 7480104 INFO @ Sat, 15 Jan 2022 21:33:28: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:33:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:33:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:33:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:33:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:33:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:33:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:33:31: 13000000 INFO @ Sat, 15 Jan 2022 21:33:31: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:33:38: 22000000 INFO @ Sat, 15 Jan 2022 21:33:39: 14000000 INFO @ Sat, 15 Jan 2022 21:33:44: 23000000 INFO @ Sat, 15 Jan 2022 21:33:46: 15000000 INFO @ Sat, 15 Jan 2022 21:33:50: 24000000 INFO @ Sat, 15 Jan 2022 21:33:54: 16000000 INFO @ Sat, 15 Jan 2022 21:33:57: 25000000 INFO @ Sat, 15 Jan 2022 21:34:01: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:34:01: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:34:01: #1 total tags in treatment: 10110051 INFO @ Sat, 15 Jan 2022 21:34:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:34:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:34:01: #1 tags after filtering in treatment: 7480104 INFO @ Sat, 15 Jan 2022 21:34:01: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:34:01: #1 finished! INFO @ Sat, 15 Jan 2022 21:34:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:34:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:34:02: 17000000 INFO @ Sat, 15 Jan 2022 21:34:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:34:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:34:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:34:09: 18000000 INFO @ Sat, 15 Jan 2022 21:34:16: 19000000 INFO @ Sat, 15 Jan 2022 21:34:23: 20000000 INFO @ Sat, 15 Jan 2022 21:34:30: 21000000 INFO @ Sat, 15 Jan 2022 21:34:37: 22000000 INFO @ Sat, 15 Jan 2022 21:34:44: 23000000 INFO @ Sat, 15 Jan 2022 21:34:51: 24000000 INFO @ Sat, 15 Jan 2022 21:34:58: 25000000 INFO @ Sat, 15 Jan 2022 21:35:03: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 21:35:03: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 21:35:03: #1 total tags in treatment: 10110051 INFO @ Sat, 15 Jan 2022 21:35:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:03: #1 tags after filtering in treatment: 7480104 INFO @ Sat, 15 Jan 2022 21:35:03: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:35:03: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8499425/SRX8499425.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling